Team:UNIPV-Pavia/Project/Results

From 2011.igem.org

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where &mu; represents the growth rate of the cells (<em>E. coli</em> MGZ1 in M9 supplemented medium) and N<sub>max</sub> represents the maximum number of cells in the well. For a detailed description of the parameters, see <a href='https://2011.igem.org/Team:UNIPV-Pavia/Project/Modelling#Table_of_parameters'>modelling section</a>. For details on parameters identification, see <a href='https://2011.igem.org/Team:UNIPV-Pavia/Project/Modelling#N'>identification section.</a>  
where &mu; represents the growth rate of the cells (<em>E. coli</em> MGZ1 in M9 supplemented medium) and N<sub>max</sub> represents the maximum number of cells in the well. For a detailed description of the parameters, see <a href='https://2011.igem.org/Team:UNIPV-Pavia/Project/Modelling#Table_of_parameters'>modelling section</a>. For details on parameters identification, see <a href='https://2011.igem.org/Team:UNIPV-Pavia/Project/Modelling#N'>identification section.</a>  
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<table class='data'>
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<table class='data'><tr><td class='row'><b> Culture </b></td><td class='row'><b> O.D.<sub>600</sub> TECAN </b></td><td class='row'><b> O.D.<sub>600</sub> Spectrophotometer </b></td><td class='row'><b> C.F.U. </b></td><td class='row'><b> Dilution Factor (10^) </b></td><td class='row'><b> N </b></td> </tr>
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<tr><td class='row'><b> Culture </b></td><td class='row><b> O.D.<sub>600</sub> TECAN </b></td><td class='row'><b> O.D.<sub>600</sub> Spectrophotometer </b></td><td class='row'><b> C.F.U. </b></td><td class='row'><b> Dilution Factor (10^) </b></td><td class='row'><b> N </b></td><td class='row'><b>
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<tr><td class='row'> C1 </td><td class='row'> 0,367950002 </td><td class='row'> 0,758004416 </td><td class='row'> 990 </td><td class='row'> 5 </td><td class='row'> 990000000 </td> </tr>
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<tr><td class='row'> C1 </td><td class='row'> 0,367950002 </td><td class='row'> 0,758004416 </td><td class='row'> 990 </td><td class='row'> 5 </td><td class='row'> 990000000 </td><td class='row'>
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<tr><td class='row'> C1 </td><td class='row'> 0,044649998 </td><td class='row'> 0,091982322 </td><td class='row'> 141 </td><td class='row'> 6 </td><td class='row'> 1410000000 </td> </tr>
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<tr><td class='row'> C1 </td><td class='row'> 0,044649998 </td><td class='row'> 0,091982322 </td><td class='row'> 141 </td><td class='row'> 6 </td><td class='row'> 1410000000 </td><td class='row'>
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<tr><td class='row'> C1 </td><td class='row'> 0,004799999 </td><td class='row'> 0,009888356 </td><td class='row'> 136 </td><td class='row'> 5 </td><td class='row'> 136000000 </td> </tr>
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<tr><td class='row'> C1 </td><td class='row'> 0,004799999 </td><td class='row'> 0,009888356 </td><td class='row'> 136 </td><td class='row'> 5 </td><td class='row'> 136000000 </td><td class='row'>
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<tr><td class='row'> C1 </td><td class='row'> 0,000549998 </td><td class='row'> 0,001133037 </td><td class='row'> 20 </td><td class='row'> 6 </td><td class='row'> 200000000 </td> </tr>
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<tr><td class='row'> C1 </td><td class='row'> 0,000549998 </td><td class='row'> 0,001133037 </td><td class='row'> 20 </td><td class='row'> 6 </td><td class='row'> 200000000 </td><td class='row'>
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<tr><td class='row'> C2 </td><td class='row'> 0,54840003 </td><td class='row'> 1,129744917 </td><td class='row'> 165 </td><td class='row'> 4 </td><td class='row'> 16500000 </td> </tr>
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<tr><td class='row'> C2 </td><td class='row'> 0,54840003 </td><td class='row'> 1,129744917 </td><td class='row'> 165 </td><td class='row'> 4 </td><td class='row'> 16500000 </td><td class='row'>
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<tr><td class='row'> C2 </td><td class='row'> 0,058200002 </td><td class='row'> 0,119896339 </td><td class='row'> 23 </td><td class='row'> 5 </td><td class='row'> 23000000 </td> </tr>
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<tr><td class='row'> C2 </td><td class='row'> 0,058200002 </td><td class='row'> 0,119896339 </td><td class='row'> 23 </td><td class='row'> 5 </td><td class='row'> 23000000 </td><td class='row'>
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<tr><td class='row'> C2 </td><td class='row'> 0,008700002 </td><td class='row'> 0,017922652 </td><td class='row'> 251 </td><td class='row'> 3 </td><td class='row'> 2510000 </td> </tr>
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<tr><td class='row'> C2 </td><td class='row'> 0,008700002 </td><td class='row'> 0,017922652 </td><td class='row'> 251 </td><td class='row'> 3 </td><td class='row'> 2510000 </td><td class='row'>
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<tr><td class='row'> C2 </td><td class='row'> 0,000100002 </td><td class='row'> 0,000206011 </td><td class='row'> 24 </td><td class='row'> 4 </td><td class='row'> 2400000 </td> </tr>
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<tr><td class='row'> C2 </td><td class='row'> 0,000100002 </td><td class='row'> 0,000206011 </td><td class='row'> 24 </td><td class='row'> 4 </td><td class='row'> 2400000 </td><td class='row'>
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Revision as of 17:00, 17 September 2011

UNIPV TEAM 2011

Results

Contents


NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C. For the cloning of the parts, E. coli TOP10 was used.

Parts assembly

Characterization of basic modules

Characterization of promoters pTet and pLux

Characterization of enzymes AiiA and LuxI

Characterization of the efficiency of RBSs from the community collection

Identification of bacterial growth parameters

The bacterial growth curve has been modelled as a logistic curve and is represented by the following equation:

dN/dt=N*μ*(Nmax-N)/Nmax
N(0)=n0

where μ represents the growth rate of the cells (E. coli MGZ1 in M9 supplemented medium) and Nmax represents the maximum number of cells in the well. For a detailed description of the parameters, see modelling section. For details on parameters identification, see identification section.

Culture O.D.600 TECAN O.D.600 Spectrophotometer C.F.U. Dilution Factor (10^) N
C1 0,367950002 0,758004416 990 5 990000000
C1 0,044649998 0,091982322 141 6 1410000000
C1 0,004799999 0,009888356 136 5 136000000
C1 0,000549998 0,001133037 20 6 200000000
C2 0,54840003 1,129744917 165 4 16500000
C2 0,058200002 0,119896339 23 5 23000000
C2 0,008700002 0,017922652 251 3 2510000
C2 0,000100002 0,000206011 24 4 2400000

Estimation of the spontaneous degradation of HSL in M9 medium and in cultures at different pH values

Characterization of BBa_T9002 biosensor

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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