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Team:Bielefeld-Germany/Data Page
From 2011.igem.org
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{{Bielefeld_2011_Header}} | {{Bielefeld_2011_Header}} | ||
| - | + | =How our System works= | |
| - | [[Image:Bielefeld_2011_S-Layer-Produktion_lysis-purification_v1.jpg|700px]] | + | [[Image:Bielefeld_2011_S-Layer-Produktion_lysis-purification_v1.jpg|center|700px|thumb|'''Fig. X: Bisphenol A degradation pathway as found in nature (picture from KEGG database).''']] |
| - | [[Image:Bielefeld_2011_Bead-Coating_v1.jpg|700px]] | + | [[Image:Bielefeld_2011_Bead-Coating_v1.jpg|center|700px|thumb|'''Fig. X: Bisphenol A degradation pathway as found in nature (picture from KEGG database).''']] |
| - | [[Image:IGEM_Bielefeld_Project.jpg|700px]] | + | [[Image:IGEM_Bielefeld_Project.jpg|center|700px|thumb|'''Fig. X: Bisphenol A degradation pathway as found in nature (picture from KEGG database).''']] |
| - | + | =Data For Our Favorite New Parts= | |
# [http://partsregistry.org/Part:BBa_K525305 Main Page] - '''Fusion Protein of S-Layer SgsE and mCitrine''': This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces. | # [http://partsregistry.org/Part:BBa_K525305 Main Page] - '''Fusion Protein of S-Layer SgsE and mCitrine''': This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces. | ||
# [http://partsregistry.org/Part:BBa_K525517 Main Page] - '''Fusion Protein of BisdA and BisdB (expressed)''': This fusion protein improves the bisphenol A degradation in ''E. coli'' compared to the so far in the partsregistry existing BPA degrading BioBricks. | # [http://partsregistry.org/Part:BBa_K525517 Main Page] - '''Fusion Protein of BisdA and BisdB (expressed)''': This fusion protein improves the bisphenol A degradation in ''E. coli'' compared to the so far in the partsregistry existing BPA degrading BioBricks. | ||
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| - | + | =Data For Pre-existing Parts= | |
# [http://partsregistry.org/Part:BBa_K123000:Experience Experience] - '''BisdA degrades Bisphenol A when used with BisdB, BBa_K123000''' (University of Alberta, iGEM 2008): Complete degradation of 120 mg L<sup>-1</sup> Bisphenol A with polycistronic ''bisdAB'' gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB. | # [http://partsregistry.org/Part:BBa_K123000:Experience Experience] - '''BisdA degrades Bisphenol A when used with BisdB, BBa_K123000''' (University of Alberta, iGEM 2008): Complete degradation of 120 mg L<sup>-1</sup> Bisphenol A with polycistronic ''bisdAB'' gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB. | ||
# [http://partsregistry.org/Part:BBa_K123001:Experience Experience] - '''BisdB degrades Bisphenol A when used with BisdA, BBa_K123001''' (University of Alberta, iGEM 2008): Complete degradation of 120 mg L<sup>-1</sup> Bisphenol A with polycistronic ''bisdAB'' gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB. | # [http://partsregistry.org/Part:BBa_K123001:Experience Experience] - '''BisdB degrades Bisphenol A when used with BisdA, BBa_K123001''' (University of Alberta, iGEM 2008): Complete degradation of 120 mg L<sup>-1</sup> Bisphenol A with polycistronic ''bisdAB'' gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB. | ||
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| - | + | =We've Also Characterized the Following Parts= | |
# [http://partsregistry.org/Part:BBa_K525405 Main Page] - '''Fusion Protein of S-Layer SbpA and mCitrine''': This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces. | # [http://partsregistry.org/Part:BBa_K525405 Main Page] - '''Fusion Protein of S-Layer SbpA and mCitrine''': This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces. | ||
# [http://partsregistry.org/Part:BBa_K525512 Main Page] - '''Polycistronic expression of BisdA and BisdB''': This is the version of BPA degrading BioBricks found in the partsregistry - comparison to our fusion protein <partinfo>K525515</partinfo>. | # [http://partsregistry.org/Part:BBa_K525512 Main Page] - '''Polycistronic expression of BisdA and BisdB''': This is the version of BPA degrading BioBricks found in the partsregistry - comparison to our fusion protein <partinfo>K525515</partinfo>. | ||
Revision as of 16:54, 17 September 2011
Contents |
How our System works
Data For Our Favorite New Parts
- [http://partsregistry.org/Part:BBa_K525305 Main Page] - Fusion Protein of S-Layer SgsE and mCitrine: This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces.
- [http://partsregistry.org/Part:BBa_K525517 Main Page] - Fusion Protein of BisdA and BisdB (expressed): This fusion protein improves the bisphenol A degradation in E. coli compared to the so far in the partsregistry existing BPA degrading BioBricks.
- [http://partsregistry.org/Part:BBa_K525710 Main Page] - NAD+-dependent DNA ligase from E. coli : This enzyme enables determination of NAD+ even in very low concentrations by coupling it with a molecular beacon based assay.
Data For Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K123000:Experience Experience] - BisdA degrades Bisphenol A when used with BisdB, BBa_K123000 (University of Alberta, iGEM 2008): Complete degradation of 120 mg L-1 Bisphenol A with polycistronic bisdAB gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB.
- [http://partsregistry.org/Part:BBa_K123001:Experience Experience] - BisdB degrades Bisphenol A when used with BisdA, BBa_K123001 (University of Alberta, iGEM 2008): Complete degradation of 120 mg L-1 Bisphenol A with polycistronic bisdAB gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB.
We've Also Characterized the Following Parts
- [http://partsregistry.org/Part:BBa_K525405 Main Page] - Fusion Protein of S-Layer SbpA and mCitrine: This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces.
- [http://partsregistry.org/Part:BBa_K525512 Main Page] - Polycistronic expression of BisdA and BisdB: This is the version of BPA degrading BioBricks found in the partsregistry - comparison to our fusion protein <partinfo>K525515</partinfo>.
