Team:UNIPV-Pavia/Instruments

From 2011.igem.org

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           <li class="toclevel-1"><a href="#Bacteria_Room_And_Molecular_Biology_Room"><span class="tocnumber">11</span> <span class="toctext">Bacteria room and Molecular Biology room</span></a></li>
           <li class="toclevel-1"><a href="#Bacteria_Room_And_Molecular_Biology_Room"><span class="tocnumber">11</span> <span class="toctext">Bacteria room and Molecular Biology room</span></a></li>
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</a><a name="Autoclave"></a>
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<a name="TECAN_Infinite_2000"></a>
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<h2> <span class="mw-headline">Autoclave</span></h2>
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<h2> <span class="mw-headline">TECAN Infinite 2000</span></h2>
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         <p align="justify"> An autoclave is a self-locking apparatus used to sterilize equipment and supplies by subjecting them to high pressure saturated steam.
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         <p align="justify"> Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates.  
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It allows steam to flow around each article placed in the chamber. The vapor penetrates cloth or paper used to package the articles being sterilized. Autoclaving brings to the destruction of all types of microorganisms, including spores. Therefore, autoclave is widely used in microbiology, medicine, veterinary science, mycology or chiropody; its size varies on the media it is sterilizing.
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          This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors.  
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The amount of time and degree of temperature necessary for sterilization depend on the articles to be sterilized and whether they are wrapped or left directly exposed to the steam. Typically the process is run at 121°C or more for 15 to 20 minutes and it is essential to ensure that all the trapped air is removed, because hot air is less efficient than steam to achieve sterility.
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          We used this instrument for the following measurement techniques: </p>
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        <ul>
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          <li> fluorescence intensity; </li>
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          <li> absorbance. </li>
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        </ul></td>
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               <td><div class="floatright"><span><img alt="Autoclave" src="https://static.igem.org/mediawiki/2010/thumb/7/7d/UNIPV_Pavia_autoclave.jpg/250px-UNIPV_Pavia_autoclave.jpg" border="0" height="188" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="TECAN Infinite 2000" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_tecan.jpg/250px-UNIPV_Pavia_tecan.jpg" border="0" height="198" width="250"></span></div></td>
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<p><br>
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<a name="Incubator_For_Plates"></a>
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<h2> <span class="mw-headline">Incubator for plates</span></h2>
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<a name="Nanodrop_ND_1000_spectrophotometer"></a>
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<h2> <span class="mw-headline">Nanodrop ND 1000 spectrophotometer</span></h2>
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         <p align="justify"> In order to grow bacteria a culture media is needed, together with plates (petri-dishes), some laboratory supplies and incubators.
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         <p align="justify"> Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample and OD in cell cultures.</p>
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The incubator is an oven able to keep bacteria at the desired temperature; the typical temperature needed to allow a rapid growth of bacteria is 37 °C.
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               <td><div class="floatright"><span><img alt="Incubator for plates" src="https://static.igem.org/mediawiki/2010/thumb/0/0a/UNIPV_Pavia_incubatorforplates.jpg/250px-UNIPV_Pavia_incubatorforplates.jpg" border="0" height="188" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="Nanodrop" src="https://static.igem.org/mediawiki/2010/thumb/2/21/UNIPV_Pavia_nanodrop.gif/150px-UNIPV_Pavia_nanodrop.gif" border="0" height="147" width="150"></span></div></td>
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<a name="Incubator_And_Shaker_For_Liquid_Cultures"></a>
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<h2> <span class="mw-headline">Incubator and shaker for liquid cultures</span></h2>
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<a name="PCR thermocyclers"></a>
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<h2> <span class="mw-headline">PCR thermocyclers</span></h2>
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         <p align="justify"> Shaking incubators, also known as environmental shakers, are often used for cell culturing, cell aeration and solubility studies. In addition to stable temperature conditions, they use an orbital agitation at variable speeds to affect the growth of cell cultures, displaced in a liquid media. All major functions (temperature, RPM and time) have alarms that alert the user to deviations from set parameters. Overtemperature protection is provided via a safety thermostat. Digital keypad operation offers the ability to calibrate the temperature controller to a reference thermometer.</p>
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         <p align="justify"> AAAAAAAAAAAAAAAAAAAAAAAAAAA </p>
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               <td><div class="floatright"><span><img alt="Incubator and shaker for liquid cultures" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_incubatorshaker.jpg/250px-UNIPV_Pavia_incubatorshaker.jpg" border="0" height="188" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="Real time" src="https://static.igem.org/mediawiki/2008/d/dd/Pv_pcr_parallel2.jpg" border="0" height="188" width="250"></span></div></td>
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         <p align="justify"> It is a laminar flow hood for handling highly toxic products. </p>
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               <td><div class="floatright"><span><img alt="Hood" src="https://static.igem.org/mediawiki/2010/thumb/d/d4/UNIPV_Pavia_hood.jpg/250px-UNIPV_Pavia_hood.jpg" border="0" height="238" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="thermocycler" src="https://static.igem.org/mediawiki/2008/0/08/Pv_pcr_parallel1.jpg" border="0" height="188" width="250"></span></div></td>
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        <p align="justify"> It is a bath in which water is heated to high temperatures for gel extraction and heath shock during bacterial transformation. </p>
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              <td><div class="floatright"><span><img alt="Water bath" src="https://static.igem.org/mediawiki/2010/thumb/4/40/UNIPV_Pavia_waterbath.jpg/250px-UNIPV_Pavia_waterbath.jpg" border="0" height="188" width="250"></span></div></td>
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<a name="Instruments_For_Electrophoresis"></a>
<a name="Instruments_For_Electrophoresis"></a>
<h2> <span class="mw-headline">Instruments for electrophoresis</span></h2>
<h2> <span class="mw-headline">Instruments for electrophoresis</span></h2>
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<a name="Gel_Image_Acquisition_System"></a>
<a name="Gel_Image_Acquisition_System"></a>
<h2> <span class="mw-headline">Gel image acquisition system</span></h2>
<h2> <span class="mw-headline">Gel image acquisition system</span></h2>
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<a name="Cold_Instruments"></a>
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<h2> <span class="mw-headline">Cold instruments</span></h2>
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<a name="Incubator_For_Plates"></a>
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<h2> <span class="mw-headline">Incubator for plates</span></h2>
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         <p align="justify"> We have freezers where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria.<br>
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         <p align="justify"> In order to grow bacteria a culture media is needed, together with plates (petri-dishes), some laboratory supplies and incubators.
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          We have also a fridge to stock plates and LB medium </p>
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The incubator is an oven able to keep bacteria at the desired temperature; the typical temperature needed to allow a rapid growth of bacteria is 37 °C.
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</p>
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               <td><div class="floatright"><span><img alt="Freezer" src="https://static.igem.org/mediawiki/2010/thumb/6/6b/UNIPV_Pavia_freezer.jpg/250px-UNIPV_Pavia_freezer.jpg" border="0" height="188" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="Incubator for plates" src="https://static.igem.org/mediawiki/2010/thumb/0/0a/UNIPV_Pavia_incubatorforplates.jpg/250px-UNIPV_Pavia_incubatorforplates.jpg" border="0" height="188" width="250"></span></div></td>
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              <td><div class="floatright"><span><img alt="Fridge" src="https://static.igem.org/mediawiki/2010/thumb/d/de/UNIPV_Pavia_fridge.jpg/250px-UNIPV_Pavia_fridge.jpg" border="0" height="188" width="250"></span></div></td>
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<a name="Nanodrop_ND_1000_spectrophotometer"></a>
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<h2> <span class="mw-headline">Nanodrop ND 1000 spectrophotometer</span></h2>
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<a name="Incubator_And_Shaker_For_Liquid_Cultures"></a>
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<h2> <span class="mw-headline">Incubator and shaker for liquid cultures</span></h2>
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         <p align="justify"> Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample and OD in cell cultures.</p>
+
         <p align="justify"> Shaking incubators, also known as environmental shakers, are often used for cell culturing, cell aeration and solubility studies. In addition to stable temperature conditions, they use an orbital agitation at variable speeds to affect the growth of cell cultures, displaced in a liquid media. All major functions (temperature, RPM and time) have alarms that alert the user to deviations from set parameters. Overtemperature protection is provided via a safety thermostat. Digital keypad operation offers the ability to calibrate the temperature controller to a reference thermometer.</p>
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               <td><div class="floatright"><span><img alt="Nanodrop" src="https://static.igem.org/mediawiki/2010/thumb/2/21/UNIPV_Pavia_nanodrop.gif/150px-UNIPV_Pavia_nanodrop.gif" border="0" height="147" width="150"></span></div></td>
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               <td><div class="floatright"><span><img alt="Incubator and shaker for liquid cultures" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_incubatorshaker.jpg/250px-UNIPV_Pavia_incubatorshaker.jpg" border="0" height="188" width="250"></span></div></td>
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<p><br>
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<a name="TECAN_Infinite_2000"></a>
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<h2> <span class="mw-headline">TECAN Infinite 2000</span></h2>
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<a name="Hood"></a>
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<h2> <span class="mw-headline">Hood</span></h2>
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         <p align="justify"> Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates.
+
         <p align="justify"> It is a laminar flow hood for handling highly toxic products. </p>
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          This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors.
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         <p></p></td>
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          We used this instrument for the following measurement techniques: </p>
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         <p></p>
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        <ul>
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          <li> fluorescence intensity; </li>
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          <li> absorbance. </li>
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               <td><div class="floatright"><span><img alt="TECAN Infinite 2000" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_tecan.jpg/250px-UNIPV_Pavia_tecan.jpg" border="0" height="198" width="250"></span></div></td>
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               <td><div class="floatright"><span><img alt="Hood" src="https://static.igem.org/mediawiki/2010/thumb/d/d4/UNIPV_Pavia_hood.jpg/250px-UNIPV_Pavia_hood.jpg" border="0" height="238" width="250"></span></div></td>
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<a name="Bacteria_Room_And_Molecular_Biology_Room"></a>
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<h2> <span class="mw-headline">Bacteria room and Molecular Biology room</span></h2>
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<!-----centrifughe>
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</a><a name="Autoclave"></a>
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<h2> <span class="mw-headline">Autoclave</span></h2>
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         <p align="justify"> The Molecular Biology room is the place in which we treat DNA.<br>
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         <p align="justify"> An autoclave is a self-locking apparatus used to sterilize equipment and supplies by subjecting them to high pressure saturated steam.
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          The Bacteria room is the place in which we work with bacteria. In this room the temperature is 37°C for growth of bacterial culture. </p>
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It allows steam to flow around each article placed in the chamber. The vapor penetrates cloth or paper used to package the articles being sterilized. Autoclaving brings to the destruction of all types of microorganisms, including spores. Therefore, autoclave is widely used in microbiology, medicine, veterinary science, mycology or chiropody; its size varies on the media it is sterilizing.
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         <p></p>
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The amount of time and degree of temperature necessary for sterilization depend on the articles to be sterilized and whether they are wrapped or left directly exposed to the steam. Typically the process is run at 121°C or more for 15 to 20 minutes and it is essential to ensure that all the trapped air is removed, because hot air is less efficient than steam to achieve sterility.
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                      Bacteria room</div>
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                  <div class="thumbinner" style="width: 252px;"><img alt="Molecular biology room" src="https://static.igem.org/mediawiki/2010/thumb/9/9c/UNIPV_Pavia_molbioroom.jpg/250px-UNIPV_Pavia_molbioroom.jpg" class="thumbimage" border="0" height="188" width="250">
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                      Molecular biology room</div>
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Revision as of 10:50, 19 September 2011

UNIPV TEAM 2011

Instruments

Contents

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TECAN Infinite 2000

Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. We used this instrument for the following measurement techniques:

  • fluorescence intensity;
  • absorbance.
TECAN Infinite 2000


Nanodrop ND 1000 spectrophotometer

Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample and OD in cell cultures.
Nanodrop


PCR thermocyclers

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Real time
thermocycler


Instruments for electrophoresis

Gel electrophoresis apparatus - Agarose gel is placed in this TBE buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is the black one while the positive is the red one. In this way DNA, that is naturally negatively charged, migrates to the positive pole.

Instruments for electrophoresis


Gel image acquisition system

It is a UV system with camera used to acquire gel run snapshots.

Gel image acquisition system


Incubator for plates

In order to grow bacteria a culture media is needed, together with plates (petri-dishes), some laboratory supplies and incubators. The incubator is an oven able to keep bacteria at the desired temperature; the typical temperature needed to allow a rapid growth of bacteria is 37 °C.

Incubator for plates


Incubator and shaker for liquid cultures

Shaking incubators, also known as environmental shakers, are often used for cell culturing, cell aeration and solubility studies. In addition to stable temperature conditions, they use an orbital agitation at variable speeds to affect the growth of cell cultures, displaced in a liquid media. All major functions (temperature, RPM and time) have alarms that alert the user to deviations from set parameters. Overtemperature protection is provided via a safety thermostat. Digital keypad operation offers the ability to calibrate the temperature controller to a reference thermometer.
Incubator and shaker for liquid cultures


Hood

It is a laminar flow hood for handling highly toxic products.

Hood