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Team:ETH Zurich
From 2011.igem.org
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| - | + | |style="font-size:2em; height: 30px" class="overview"|SmoColi | |
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| - | == | + | |'''SmoColi is a bacterio-quantifier of acetaldehyde concentration that can be used as a passive smoke detector. Acetaldehyde is a toxic and carcinogenic component of cigarette smoke. It has a boiling point of 20.2 °C and is very volatile, thus can be used as an information carrier through air. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which an acetaldehyde gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense acetaldehyde by a synthetically re-designed fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information about acetaldehyde. Finally, if the acetaldehyde concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red.''' |
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| - | SmoColi is a bacterio-quantifier of acetaldehyde concentration that can be used as a passive smoke detector. Acetaldehyde is a toxic and carcinogenic component of cigarette smoke. It has a boiling point of 20.2 °C and is very volatile, thus can be used as an information carrier through air. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which an acetaldehyde gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense acetaldehyde by a synthetically re-designed fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information about acetaldehyde. Finally, if the acetaldehyde concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red. | + | |
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Revision as of 16:25, 12 September 2011
| SmoColi |
| SmoColi is a bacterio-quantifier of acetaldehyde concentration that can be used as a passive smoke detector. Acetaldehyde is a toxic and carcinogenic component of cigarette smoke. It has a boiling point of 20.2 °C and is very volatile, thus can be used as an information carrier through air. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which an acetaldehyde gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense acetaldehyde by a synthetically re-designed fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information about acetaldehyde. Finally, if the acetaldehyde concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red. |
