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SEPTEMBER: WEEK 2
September, 5th
T9002-ENTERO and ENTERO-4C5 were diluted 1:200 and 1:800 to perform two different tests on supernatants (collected on August, 31st, from cultures and media at different pHs, and on September, 3rd, from cultures expressing AiiA enzyme in high copy number plasmids).
September, 6th
T9002-ENTERO and ENTERO-RBS were diluted 1:200 in a final volume of 1 ml and 18 ml respectively. After two hours 190 μl were aliquoted in 96-well microplate and induced with 10 μl of supernatants and 3OC6-HSL known concentrations.
September, 7thSeptember, 8thSeptember, 9thSeptember, 10thSeptember, 11th
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Team:UNIPV-Pavia/Calendar/September/week2
From 2011.igem.org
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After two hours they were induced with 100 ng/ml atc and after one hour 3OC<small><sub>6</sub></small>-HSL was added at a final concentration of 100 nM. At this time the first sample was collected from each tube; samples were also collected after three hours, five hours and twenty-four hours. | After two hours they were induced with 100 ng/ml atc and after one hour 3OC<small><sub>6</sub></small>-HSL was added at a final concentration of 100 nM. At this time the first sample was collected from each tube; samples were also collected after three hours, five hours and twenty-four hours. | ||
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- | + | E3N-1C3 plate was grown; eight colonies from this plate were picked while three colonies were picked from a older plate. All of these colonies were PCR-screened and grown in LB + Cm34. | |
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Revision as of 14:13, 6 September 2011