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Team:ETH Zurich/Biology/Journal
From 2011.igem.org
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{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif; margin-top:25px; border-radius:10px;" cellspacing="15" cellpadding="5" | {| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif; margin-top:25px; border-radius:10px;" cellspacing="15" cellpadding="5" | ||
|style="font-size:2em;" valign="top" class="biology"|Lab Journal | |style="font-size:2em;" valign="top" class="biology"|Lab Journal | ||
| - | |style="font-family: helvetica, arial, sans-serif;font-size:1.5em; border-radius:10px" align="right" class="biologyTitle"|Week [[#|1]] [[#|2]] [[#|3]] [[#|4]] [[#|5]] [[#|6]] [[#|7]] [[#|8]] [[#|9]] [[#|10]] [[#|11]] [[#|12]] [[#|13]] [[#|14]] | + | |style="font-family: helvetica, arial, sans-serif;font-size:1.5em; border-radius:10px" align="right" class="biologyTitle"|Week [[#Week 1: 20.6-26.6|1]] [[#Week 2: 27.6-3.7|2]] [[#Week 3: 4.7-10.7|3]] [[#Week 4: 11.7-17.7|4]] [[#Week 5: 18.7-24.7|5]] [[#Week 6: 25.7-31.7|6]] [[#Week 7: 1.8-7.8|7]] [[#Week 8: 8.8-14.8|8]] [[#Week 9: 15.8-21.8|9]] [[#Week 10: 22.8-28.8|10]] [[#Week 11: 29.8-4.9|11]] [[#Week 12: 5.9.-11.9|12]] [[#Week 13: 12.9.-18.9|13]] [[#Week 14: 19.9.-25.9|14]] |
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|colspan="2"|'''Here you can follow the progress of our project as it is coming together.''' | |colspan="2"|'''Here you can follow the progress of our project as it is coming together.''' | ||
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*Preparation of chemically competent DH5-α and JM101 cells | *Preparation of chemically competent DH5-α and JM101 cells | ||
| - | == '''Week 11: 29.8-4.9 | + | == '''Week 11: 29.8-4.9''' == |
* Transformation and Ligation | * Transformation and Ligation | ||
** P<sub>tet</sub>-CI and Terminator | ** P<sub>tet</sub>-CI and Terminator | ||
| Line 98: | Line 98: | ||
* Test of the improved AlcR system | * Test of the improved AlcR system | ||
| - | == '''Week 12: 5.9.-11.9 | + | == '''Week 12: 5.9.-11.9''' == |
*Design of a system to characterize our new biobrick LacI<sub>M1</sub> | *Design of a system to characterize our new biobrick LacI<sub>M1</sub> | ||
**Transformation and Ligation | **Transformation and Ligation | ||
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*Testdigest of λP-luxI-Terminator | *Testdigest of λP-luxI-Terminator | ||
| - | == '''Week 13: 12.9.-18.9 | + | == '''Week 13: 12.9.-18.9''' == |
== '''Week 14: 19.9.-25.9''' == | == '''Week 14: 19.9.-25.9''' == | ||
Revision as of 08:29, 6 September 2011
| Lab Journal | Week 1 2 3 4 5 6 7 8 9 10 11 12 13 14 |
| Here you can follow the progress of our project as it is coming together. | |
Week 1: 20.6-26.6
- First meeting
- Brainstorming
Week 2: 27.6-3.7
- Brainstorming
Week 3: 4.7-10.7
- Working on the design of the system
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 |
- Design of several Operons for AlcR and several PCR primer
Week 4: 11.7-17.7
- Ordering of the LacIM1 and codonoptimized AlcR gene
- Making of competent DH5-α cells
- Cloning of the AlcR-testsystem
Week 5: 18.7-24.7
- First test of the AlcR-system in 96-well plates
Week 6: 25.7-31.7
- Transformation of the parts from the iGEM plates
Week 7: 1.8-7.8
- Growth test of DH5-α with AlcR-system in flasks
- Ligation and Transformation of
- λP and luxI
- λP and lacI
- Plac and GFPLVA
- Plux and mCherry
- Ptet and CI -had to be redone because of point mutation in the primer (week 9)
- Pconst and luxR
- First meeting with Dr. Oliver Frey (Bio engineering laboratory, Prof. Andreas Hierlemann, D-BSSE ETHZ) to exchange ideas about microfluidic design
Week 8: 8.8-14.8
- Synthesized LacIM1 Gen arrived
- Ligation and Transformation of
- Plac-GFPLVA and Terminator
- Plux-mCherry and Terminator
- Pconst-luxR and Terminator
- Plac-GFPLVA-Terminator and Plux-mCherry-Terminator on one plasmid
- Ptet and LacIM1
- Ptet-LacIM1 and Terminator
- Improving of the testsystem
- Exchange of same rare codons in AlcR by PCR
- His-tagging of AlcR
- GFP assam
- Transformation of λP
- Making of competent JM101 cells
- Check for transformation efficiency
Week 9: 15.8-21.8
- Growth and repression test of AlcR-system with M9-medium
- Ligation and Transformation of
- λP and lacI
- λP and luxI
- Ptet and CI
- λP-lacI and terminator
- λP-luxI and terminator
Week 10: 22.8-28.8
- Transformation and Ligation
- Ptet-CI and Terminator
- Preparation of chemically competent DH5-α and JM101 cells
Week 11: 29.8-4.9
- Transformation and Ligation
- Ptet-CI and Terminator
- Diffusion test through tube system filled with agarose and cells
- Test of the improved AlcR system
Week 12: 5.9.-11.9
- Design of a system to characterize our new biobrick LacIM1
- Transformation and Ligation
- PTet and LacI
- PLac and GFP assam
- Transformation and Ligation
- Design of an additional construct to test the band pass filter
- Transformation and Ligation
- Pconst and TetRLVA
- Transformation and Ligation
- Growth-test of E.coli in M9
- Testdigest of λP-luxI-Terminator
