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SEPTEMBER: WEEK 1
September, 2ndE3-1C3-2 was purified in order to screen the part length.
R0040 in J61002-1C3, E3N-1C3 and J101-31N-1C3 were transformed in 200 μl of TOP10 competent cells.
September, 3rd
All the cultures were diluted 1:200 in M9 with the proper antibiotic. After two hours T9002-ENTERO and ENTERO-RBS were aliquoted in 96-well microplate and induced with known concentrations of 3OC6-HSL and with the supernatants collected on August, 31st.
The gel did not show the correct band.
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Team:UNIPV-Pavia/Calendar/September/week1
From 2011.igem.org
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Inoculum of E24-2, E25-1, E26-2, E27-2 and RBS32 in M9 + Amp to collect supernatant after 3OC<small><sub>6</sub></small>-HSL supplementation. | Inoculum of E24-2, E25-1, E26-2, E27-2 and RBS32 in M9 + Amp to collect supernatant after 3OC<small><sub>6</sub></small>-HSL supplementation. | ||
<br> | <br> | ||
- | Inoculum in M9 + Cm12.5 of T9002-ENTERO and ENTERO-RBS to measure 3OC<small><sub>6</sub></small>-HSL concentration in supernatants at different pHs collected on <a | + | Inoculum in M9 + Cm12.5 of T9002-ENTERO and ENTERO-RBS to measure 3OC<small><sub>6</sub></small>-HSL concentration in supernatants at different pHs collected on <a href="https://2011.igem.org/Team:UNIPV-Pavia/Calendar/August/week5#August.2C_31st">August, 31st</a>. |
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Revision as of 10:42, 4 September 2011