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Team:Paris Liliane Bettencourt/Notebook/2011/08/31/
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==Axel== | ==Axel== | ||
| - | ===extraction of genomic DNA (CodY-)=== | + | ===extraction of genomic DNA (CodY-) : done=== |
TECAN quantification of DNA: OD260=47,9ng/µL | TECAN quantification of DNA: OD260=47,9ng/µL | ||
Latest revision as of 20:49, 28 October 2011
Contents |
Axel
extraction of genomic DNA (CodY-) : done
TECAN quantification of DNA: OD260=47,9ng/µL
Camille
The sequencing results arrived and it appeared that only the cloning of the promoter Hyperspank in pSB1C3 succeeded. I then launched the clons in liquid culture for glycerols it would be the strain S87.
Mathias & Laura
We tried to insert the Biobricks "LacI controled T7 and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3
Miniprep
-LacI controled T7 and reporter for Bacillus
-T7 Autoamplifier system + Reporter
-pHM3
Digestion and gel extraction
-LacI controled T7 and reporter for Bacillus by EcoR1 and Pst1
-T7 Autoamplifier system + Reporter by EcoR1 and Pst1
-pHM3 by EcoR1 and Pst1
Ligation and transformation
-LacI controled T7 and reporter for Bacillus & pHM3
-T7 Autoamplifier system + Reporter & pHM3
