Team:Osaka/week5

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(Difference between revisions)

Latest revision as of 05:52, 19 October 2011

August 28 (Sun)

Transfer to liquid culture:09,10,11
spectrophotometry of 04

August 29 (Mon)

iGEM Japan Meet-Up at Kyoto University

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Gel electrophoresis of digests
- 09 ok
- 10 ok
- 11 ×
Transformaton of Registry parts (See Table 6).

Table6
ID	Part Name	Resistance	Description
3-10C	<bbpart>BBa_K115056</bbpart>	AC	IPP -> OPP or DMAPP -> OPP

August 30 (Tue)

To liquid culture:11,3-10C

August 31 (Wed)

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Gel electrophoresis of digests
- 11,3-10C ok
Ligation
- 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
- 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
- 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
- 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
- 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
Transformation of ligation products

September 1 (Thu)

Transfer to liquid culture:12,13,14,15,16

September 2 (Fri)

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Gel electrophoresis of digests
- 12,13,15 ok
- 14,16 ×
Ligation
- 17(A):15(upstream)+06(downstream)+1-1G(vector)
Transfer to liquid culture:03,04,3-10C

September 3 (Sat)

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Gel electrophoresis of digests
- 3-10C × deactivation of SpeⅠ?
Inoculation of 03,04

Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

@@ Line 1: / Line 1: @@
 {{Osaka}}
+__NOTOC__
+==August 28 (Sun)==
-==August 28(Sun)==
 # Transfer to liquid culture:09,10,11
 #spectrophotometry of 04
-==August 29(Mon)==
+==August 29 (Mon)==
   iGEM Japan Meet-Up at Kyoto University
@@ Line 18: / Line 17: @@
 # Transformaton of Registry parts (See Table 6).
 {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
-|+Table2
+|+Table6
 !ID!!Part Name!!Resistance!!Description
 |-
-|3-10C||<bbpart>BBa_K115056</bbpart>||AC||
+|3-10C||<bbpart>BBa_K115056</bbpart>||AC|| IPP -> OPP or DMAPP -> OPP
 |}
-==August 30(Tue)==
+==August 30 (Tue)==
+# To liquid culture:11,3-10C
+==August 31 (Wed)==
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Gel electrophoresis of digests
+#* 11,3-10C  ok
+# Ligation
+#* 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
+#* 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
+#* 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
+#* 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
+#* 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
+# Transformation of ligation products
-==August 31(Wed)==
+==September 1 (Thu)==
+# Transfer to liquid culture:12,13,14,15,16
+==September 2 (Fri)==
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Gel electrophoresis of digests
+#* 12,13,15  ok
+#* 14,16  ×
+# Ligation
+#* 17(A):15(upstream)+06(downstream)+1-1G(vector)
+# Transfer to liquid culture:03,04,3-10C
-==September 1(Thu)==
+==September 3 (Sat)==
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Gel electrophoresis of digests
+#* 3-10C ×  deactivation of SpeⅠ?
+# Inoculation of 03,04
-==September 2(Fri)==
+[[Team:Osaka/Notebook|Back to Notebook]]
-==September 3(Sat)==
+week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|'''5'''|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]