Team:UNIPV-Pavia/Freezer

From 2011.igem.org

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<html>
<html>
  <h2 class="art-postheader">
  <h2 class="art-postheader">
-
FREEZER
+
Freezer Management
</h2>
</h2>
<div class="cleared"></div>
<div class="cleared"></div>
<div class="art-postcontent">
<div class="art-postcontent">
-
 
<br>
<br>
 +
<div align="center">
<div align="center">
-
<p>For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look <a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/October/settimana3#October.2C_18th" class="external text" title="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/October/settimana3#October.2C_18th" rel="nofollow">here</a>.
+
<p>For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look <a href="https://2011.igem.org/Team:UNIPV-Pavia/Parts/Submitted#freezer" >here</a>.
-
</p>
+
</p><br>
</div>
</div>
Line 30: Line 30:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">969</td>
+
       <td class="row">969<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 42: Line 42:
       <td class="row">(aiiA) autoinducer inactivation enzyme</td>
       <td class="row">(aiiA) autoinducer inactivation enzyme</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">789+25<sup>*</sup></td>
+
       <td class="row">789<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 78: Line 78:
       <td class="row">(luxI) autoinducer synthetase for AHL</td>
       <td class="row">(luxI) autoinducer synthetase for AHL</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">618+25<sup>*</sup></td>
+
       <td class="row">618<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 90: Line 90:
       <td class="row">(mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">(mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">requested from the Registry</td>
       <td class="row">requested from the Registry</td>
-
       <td class="row">843 (25 included)<sup>*</sup></td>
+
       <td class="row">843<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1AK3</td>
       <td class="row">pSB1AK3</td>
Line 100: Line 100:
   <tr>
   <tr>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></td>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></td>
-
       <td class="row">RBS30 (eff=0.6) (SCAR BEFORE: 8bp)</td>
+
       <td class="row">RBS30 (eff=0.6)</td>
       <td class="row">2009</td>
       <td class="row">2009</td>
       <td class="row">15</td>
       <td class="row">15</td>
Line 125: Line 125:
   <tr>
   <tr>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0032">BBa_B0032</a></td>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0032">BBa_B0032</a></td>
-
       <td class="row">RBS32 (eff=0.3)</td>
+
       <td class="row">RBS32 (eff=0.3) / also used as non-fluorescent control in TECAN measurements (RBS)</td>
       <td class="row">2009</td>
       <td class="row">2009</td>
       <td class="row">13</td>
       <td class="row">13</td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10 / E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (MIT)</td>
       <td class="row">Seq ok (MIT)</td>
Line 151: Line 151:
       <td class="row">(pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP</td>
       <td class="row">(pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP</td>
       <td class="row">2010</td>
       <td class="row">2010</td>
-
       <td class="row">923(25 included)<sup>*</sup></td>
+
       <td class="row">923<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A3</td>
       <td class="row">pSB1A3</td>
Line 164: Line 164:
       <td class="row">(RBS34-luxI) AHL-making Enzyme, luxI (+RBS)</td>
       <td class="row">(RBS34-luxI) AHL-making Enzyme, luxI (+RBS)</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">661 (25 included)<sup>*</sup></td>
+
       <td class="row">661<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 175: Line 175:
       <td class="row">(RBS34-mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">(RBS34-mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">2009</td>
       <td class="row">2009</td>
-
       <td class="row">861 (25 included)<sup>*</sup></td>
+
       <td class="row">861<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 204: Line 204:
       <td class="row">Seq ok (MIT)</td>
       <td class="row">Seq ok (MIT)</td>
       <td class="row">30/06/2011</td>
       <td class="row">30/06/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row"><a href="http://partsregistry.org/Part:BBa_K300005">BBa_K3000005</a></td>
 +
      <td class="row">GFP (Silver Standard Prefix) with terminator</td>
 +
      <td class="row">2010</td>
 +
      <td class="row">854</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Gemonics)</td>
 +
      <td class="row">FREEZER 2010 Ligations</td>
 +
  </tr>
 +
 +
 +
  <tr>
 +
      <td class="row"><a href="http://partsregistry.org/Part:BBa_K112808">BBa_K112808</a> in <a href="http://partsregistry.org/Part:pSB4C5">pSB4C5</a></td>
 +
      <td class="row">Enterobacteria phage T4 Lysis Device - no promoter / also used as non-fluorescent control in TECAN measurements (ENTERO4C5)</td>
 +
      <td class="row">2009</td>
 +
      <td class="row">1785</td>
 +
      <td class="row">E. coli TOP10 / E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Gemonics)</td>
 +
      <td class="row">FREEZER 2009 Ligations</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">T9002</td>
 +
      <td class="row"><a href="http://partsregistry.org/Part:BBa_T9002">BBa_T9002</a></td>
 +
      <td class="row">2009</td>
 +
      <td class="row">1945</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (MIT)</td>
 +
      <td class="row">2009 Registry Distribution</td>
 +
  </tr>
 +
 +
 +
  <tr>
 +
      <td class="row">ENTERO-RBS</td>
 +
      <td class="row"><a href="http://partsregistry.org/Part:BBa_K112808">BBa_K112808</a> in <a href="http://partsregistry.org/Part:pSB4C5">pSB4C5</a> cotransformed with <a href="http://partsregistry.org/Part:BBa_B0032">BBa_B0032</a> / also used as non-fluorescent control in TECAN measurements</td>
 +
      <td class="row">2011</td>
 +
      <td class="row">1785 - 13</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2 - pSB4C5</td>
 +
      <td class="row">Seq ok</td>
 +
      <td class="row">FREEZER 2011 Ligations</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">T9002-ENTERO</td>
 +
      <td class="row"><a href="http://partsregistry.org/Part:BBa_T9002">BBa_T9002</a> cotransformed with <a href="http://partsregistry.org/Part:BBa_K112808">BBa_K112808</a> in <a href="http://partsregistry.org/Part:pSB4C5">pSB4C5</a> / used as measurement device in TECAN measurements</td>
 +
      <td class="row">2011</td>
 +
      <td class="row">1945 - 1785</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2 - pSB4C5</td>
 +
      <td class="row">Seq ok</td>
 +
      <td class="row">FREEZER 2011 Ligations</td>
   </tr>
   </tr>
Line 227: Line 284:
       <td class="row">aiiA-TT</td>
       <td class="row">aiiA-TT</td>
       <td class="row">C0060(E-S)+B0015(E-X)</td>
       <td class="row">C0060(E-S)+B0015(E-X)</td>
-
       <td class="row">926+25<sup>*</sup></td>
+
       <td class="row">951<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1AK3</td>
       <td class="row">pSB1AK3</td>
Line 238: Line 295:
       <td class="row">RBS30-LuxI</td>
       <td class="row">RBS30-LuxI</td>
       <td class="row">C0061(X-P)+B0030(S-P)</td>
       <td class="row">C0061(X-P)+B0030(S-P)</td>
-
       <td class="row">639+25<sup>*</sup></td>
+
       <td class="row">664<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 249: Line 306:
       <td class="row">RBS31-LuxI</td>
       <td class="row">RBS31-LuxI</td>
       <td class="row">C0061(X-P)+B0031(S-P)</td>
       <td class="row">C0061(X-P)+B0031(S-P)</td>
-
       <td class="row">638+25<sup>*</sup></td>
+
       <td class="row">663<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 260: Line 317:
       <td class="row">RBS32-LuxI</td>
       <td class="row">RBS32-LuxI</td>
       <td class="row">C0061(X-P)+B0032(S-P)</td>
       <td class="row">C0061(X-P)+B0032(S-P)</td>
-
       <td class="row">637+25<sup>*</sup></td>
+
       <td class="row">662<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
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       <td class="row">RBS30-mRFP-TT</td>
       <td class="row">RBS30-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0030(S-P)</td>
       <td class="row">I13501(X-P)+B0030(S-P)</td>
-
       <td class="row">864(25 included)<sup>*</sup></td>
+
       <td class="row">864<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 282: Line 339:
       <td class="row">RBS31-mRFP-TT</td>
       <td class="row">RBS31-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0031(S-P)</td>
       <td class="row">I13501(X-P)+B0031(S-P)</td>
-
       <td class="row">863(25 included)<sup>*</sup></td>
+
       <td class="row">863<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
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       <td class="row">RBS32-mRFP-TT</td>
       <td class="row">RBS32-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0032(S-P)</td>
       <td class="row">I13501(X-P)+B0032(S-P)</td>
-
       <td class="row">862(25 included)<sup>*</sup></td>
+
       <td class="row">862<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 304: Line 361:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">K081022(E-P)+4C5(E-P)</td>
       <td class="row">K081022(E-P)+4C5(E-P)</td>
-
       <td class="row">969(25 included)<sup>*</sup></td>
+
       <td class="row">969<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 315: Line 372:
       <td class="row">RBS30-aiiA-TT</td>
       <td class="row">RBS30-aiiA-TT</td>
       <td class="row">E1(X-P)+B0030(S-P)</td>
       <td class="row">E1(X-P)+B0030(S-P)</td>
-
       <td class="row">947+25<sup>*</sup></td>
+
       <td class="row">972<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 326: Line 383:
       <td class="row">RBS31-aiiA-TT</td>
       <td class="row">RBS31-aiiA-TT</td>
       <td class="row">E1(X-P)+B0031(S-P)</td>
       <td class="row">E1(X-P)+B0031(S-P)</td>
-
       <td class="row">946+25<sup>*</sup></td>
+
       <td class="row">971<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 337: Line 394:
       <td class="row">RBS32-aiiA-TT</td>
       <td class="row">RBS32-aiiA-TT</td>
       <td class="row">E1(X-P)+B0032(S-P)</td>
       <td class="row">E1(X-P)+B0032(S-P)</td>
-
       <td class="row">945+25<sup>*</sup></td>
+
       <td class="row">970<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">14/07/2011</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
      <td class="row">E12-1</td>
 +
      <td class="row">RBS34-aiiA-TT</td>
 +
      <td class="row">E1(X-P)+ B0034(S-P)</td>
 +
      <td class="row">969<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 348: Line 416:
       <td class="row">pTet-RBS30-LuxI</td>
       <td class="row">pTet-RBS30-LuxI</td>
       <td class="row">E2(X-P)+R0040(S-P)</td>
       <td class="row">E2(X-P)+R0040(S-P)</td>
-
       <td class="row">701+25<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 359: Line 427:
       <td class="row">pTet-RBS31-LuxI</td>
       <td class="row">pTet-RBS31-LuxI</td>
       <td class="row">E3(X-P)+R0040(S-P)</td>
       <td class="row">E3(X-P)+R0040(S-P)</td>
-
       <td class="row">700+25<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row"></td>
       <td class="row"></td>
-
       <td class="row">13/07/2011</td>
+
       <td class="row">FAILED</td>
   </tr>
   </tr>
Line 370: Line 438:
       <td class="row">pTet-RBS32-LuxI</td>
       <td class="row">pTet-RBS32-LuxI</td>
       <td class="row">E4(X-P)+R0040(S-P)</td>
       <td class="row">E4(X-P)+R0040(S-P)</td>
-
       <td class="row">699+25<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row"></td>
       <td class="row"></td>
-
       <td class="row">13/07/2011</td>
+
       <td class="row">FAILED</td>
   </tr>
   </tr>
Line 381: Line 449:
       <td class="row">pTet-RBS34-LuxI</td>
       <td class="row">pTet-RBS34-LuxI</td>
       <td class="row">C0261(X-P)+R0040(S-P)</td>
       <td class="row">C0261(X-P)+R0040(S-P)</td>
-
       <td class="row">698??+25 (già 723)<sup>*</sup></td>
+
       <td class="row">723<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 392: Line 460:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
       <td class="row">E5(X-P)+E8(S-P)</td>
       <td class="row">E5(X-P)+E8(S-P)</td>
-
       <td class="row">1841(25 included)<sup>*</sup></td>
+
       <td class="row">1841<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 403: Line 471:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
       <td class="row">E6(X-P)+E8(S-P)</td>
       <td class="row">E6(X-P)+E8(S-P)</td>
-
       <td class="row">1840(25 included)<sup>*</sup></td>
+
       <td class="row">1840<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 414: Line 482:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
       <td class="row">E7(X-P)+E8(S-P)</td>
       <td class="row">E7(X-P)+E8(S-P)</td>
-
       <td class="row">1839(25 included)<sup>*</sup></td>
+
       <td class="row">1839<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 425: Line 493:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
       <td class="row">I13507(X-P)+E8(S-P)</td>
       <td class="row">I13507(X-P)+E8(S-P)</td>
-
       <td class="row">1838(25 included)<sup>*</sup></td>
+
       <td class="row">1838<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">13/07/2011</td>
       <td class="row">13/07/2011</td>
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E17-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
 
-
      <td class="row">E5(X-P)+E8(S-P)</td>
 
-
      <td class="row">1839<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E18-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
 
-
      <td class="row">E6(X-P)+E8(S-P)</td>
 
-
      <td class="row">1840<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E19-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
 
-
      <td class="row">E7(X-P)+E8(S-P)</td>
 
-
      <td class="row">1839<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E20-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
 
-
      <td class="row">I13507(X-P)+E8(S-P)</td>
 
-
      <td class="row">1838<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
   </tr>
   </tr>
Line 480: Line 504:
       <td class="row">pTet-RBS30-mRFP-TT</td>
       <td class="row">pTet-RBS30-mRFP-TT</td>
       <td class="row">E5(X-P)+R0040(S-P)</td>
       <td class="row">E5(X-P)+R0040(S-P)</td>
-
       <td class="row">926(25 included)<sup>*</sup></td>
+
       <td class="row">926<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 491: Line 515:
       <td class="row">pTet-RBS31-mRFP-TT</td>
       <td class="row">pTet-RBS31-mRFP-TT</td>
       <td class="row">E6(X-P)+R0040(S-P)</td>
       <td class="row">E6(X-P)+R0040(S-P)</td>
-
       <td class="row">925(25 included)<sup>*</sup></td>
+
       <td class="row">925<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 502: Line 526:
       <td class="row">pTet-RBS32-mRFP-TT</td>
       <td class="row">pTet-RBS32-mRFP-TT</td>
       <td class="row">E7(X-P)+R0040(S-P)</td>
       <td class="row">E7(X-P)+R0040(S-P)</td>
-
       <td class="row">924(25 included)<sup>*</sup></td>
+
       <td class="row">924<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">14/07/2011</td>
       <td class="row">14/07/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">
 +
<a href="http://partsregistry.org/wiki/index.php/Part:BBa_R0040">BBa_R0040</a> in <a href="http://partsregistry.org/wiki/index.php/Part:BBa_J61002">BBa_J61002</a></td>
 +
      <td class="row">pTet in BBa_J61002 plasmid</td>
 +
      <td class="row">BBa_R0040(S-P)+BBa_J23101(S-P)</td>
 +
      <td class="row">923<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10/ E. coli MGZ1</td>
 +
      <td class="row">BBa_J61002</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">15/07/2011</td>
   </tr>
   </tr>
Line 513: Line 549:
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
-
       <td class="row">1009+25<sup>*</sup></td>
+
       <td class="row">1034<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 524: Line 560:
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
-
       <td class="row">1008+25<sup>*</sup></td>
+
       <td class="row">1033<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 535: Line 571:
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
-
       <td class="row">1007+25<sup>*</sup></td>
+
       <td class="row">1032<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 546: Line 582:
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
-
       <td class="row">1006+25<sup>*</sup></td>
+
       <td class="row">1031<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 557: Line 593:
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">701+25<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
-
       <td class="row">Seq ok (BMR Genomics)</td>
+
       <td class="row">Bad Seq (BMR Genomics)</td>
       <td class="row">21/07/2011</td>
       <td class="row">21/07/2011</td>
   </tr>
   </tr>
Line 568: Line 604:
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">700+25<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row"></td>
       <td class="row"></td>
-
       <td class="row">???</td>
+
       <td class="row">FAILED</td>
   </tr>
   </tr>
Line 579: Line 615:
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">699+25<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row"></td>
       <td class="row"></td>
-
       <td class="row">???</td>
+
       <td class="row">FAILED</td>
   </tr>
   </tr>
Line 590: Line 626:
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">698+25<sup>*</sup></td>
+
       <td class="row">723<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 601: Line 637:
       <td class="row">pTet-RBS30-mRFP-TT in pSB4C5</td>
       <td class="row">pTet-RBS30-mRFP-TT in pSB4C5</td>
       <td class="row">E21(E-P)+pSB4C5(E-P)</td>
       <td class="row">E21(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">924<sup>*</sup></td>
+
       <td class="row">926<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 648: Line 684:
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
-
       <td class="row">Red colonies</td>
+
       <td class="row">Length ok</td>
       <td class="row">25/07/2011</td>
       <td class="row">25/07/2011</td>
   </tr>
   </tr>
Line 656: Line 692:
       <td class="row">pTet-RBS30-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS30-aiiA-TT in pSB4C5</td>
       <td class="row">E24(E-P)+pSB4C5(E-P)</td>
       <td class="row">E24(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1007<sup>*</sup></td>
+
       <td class="row">1034<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
-
       <td class="row"></td>
+
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">29/07/2011</td>
       <td class="row">29/07/2011</td>
   </tr>
   </tr>
Line 667: Line 703:
       <td class="row">pTet-RBS31-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS31-aiiA-TT in pSB4C5</td>
       <td class="row">E25(E-P)+pSB4C5(E-P)</td>
       <td class="row">E25(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1008<sup>*</sup></td>
+
       <td class="row">1033<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 678: Line 714:
       <td class="row">pTet-RBS32-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS32-aiiA-TT in pSB4C5</td>
       <td class="row">E26(E-P)+pSB4C5(E-P)</td>
       <td class="row">E26(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1007<sup>*</sup></td>
+
       <td class="row">1032<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 689: Line 725:
       <td class="row">pTet-RBS34-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS34-aiiA-TT in pSB4C5</td>
       <td class="row">E27(E-P)+pSB4C5(E-P)</td>
       <td class="row">E27(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1006<sup>*</sup></td>
+
       <td class="row">1031<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 697: Line 733:
   <tr>
   <tr>
-
       <td class="row">E41N1</td>
+
       <td class="row">E41N-1</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+E3(X-P)</td>
       <td class="row">E36(S-P)+E3(X-P)</td>
-
       <td class="row">700<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
-
       <td class="row"></td>
+
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">04/08/2011</td>
       <td class="row">04/08/2011</td>
   </tr>
   </tr>
Line 711: Line 747:
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+ E4(X-P)</td>
       <td class="row">E36(S-P)+ E4(X-P)</td>
-
       <td class="row">699<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">29/07/2011</td>
       <td class="row">29/07/2011</td>
 +
  </tr>
   <tr>
   <tr>
-
       <td class="row">E43-?</td>
+
       <td class="row">E43-3</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+ E2(X-P)</td>
       <td class="row">E36(S-P)+ E2(X-P)</td>
-
       <td class="row">701<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
-
       <td class="row">???</td>
+
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">25/08/2011</td>
       <td class="row">25/08/2011</td>
   </tr>
   </tr>
 +
 +
  <tr>
 +
      <td class="row">E44-1</td>
 +
      <td class="row">pTet-RBS34-mRFP-TT in pSB1C3</td>
 +
      <td class="row"><a href="http://partsregistry.org/wiki/index.php/Part:BBa_R0040">BBa_R0040</a> in <a href="http://partsregistry.org/wiki/index.php/Part:BBa_J61002">BBa_J61002</a> (E-P) + pSB1C3 (E-P)</td>
 +
      <td class="row">923<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">04/09/2011</td>
   </tr>
   </tr>
 +
 +
 +
  <tr>
 +
      <td class="row">J101-E5</td>
 +
      <td class="row">J23101-RBS30-mRFP-TT in pSB4C5</td>
 +
      <td class="row">BBa_J23101(S-P)+ E5(S-P)</td>
 +
      <td class="row">905*</sup></td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">02/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">J101-31</td>
 +
      <td class="row">J23101-RBS31-mRFP-TT in pSB4C5</td>
 +
      <td class="row">BBa_J23101(S-P)+ E6(S-P)</td>
 +
      <td class="row">904*</sup></td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">02/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">J101-E7</td>
 +
      <td class="row">J23101-RBS32-mRFP-TT in pSB4C5</td>
 +
      <td class="row">BBa_J23101(S-P)+ E7(S-P)</td>
 +
      <td class="row">903*</sup></td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">02/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">J101-4C5</td>
 +
      <td class="row">J23101-RBS34-mRFP-TT in pSB4C5</td>
 +
      <td class="row">BBa_J23101(E-P)+ pSB4C5(E-P)</td>
 +
      <td class="row">902*</sup></td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">02/08/2011</td>
 +
  </tr>
 +
 +
 +
 +
 +
  <tr>
 +
      <td class="row">E3O-1C3-3</td>
 +
      <td class="row">RBS31-LuxI</td>
 +
      <td class="row">E3-1(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">663<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">07/09/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E4N-1C3-3</td>
 +
      <td class="row">RBS32-LuxI</td>
 +
      <td class="row">E4-2(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">662<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">11/09/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E5-1C3</td>
 +
      <td class="row">RBS30-mRFP-TT</td>
 +
      <td class="row">E5-2(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">864<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E6-1C3</td>
 +
      <td class="row">RBS31-mRFP-TT</td>
 +
      <td class="row">E6-1(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">863<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E7-1C3</td>
 +
      <td class="row">RBS32-mRFP-TT</td>
 +
      <td class="row">E7-2(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">862<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E9-1C3</td>
 +
      <td class="row">RBS30-aiiA-TT</td>
 +
      <td class="row">E9-2(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">972<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E10-1C3</td>
 +
      <td class="row">RBS31-aiiA-TT</td>
 +
      <td class="row">E10-1(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">971<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E11-1C3</td>
 +
      <td class="row">RBS32-aiiA-TT</td>
 +
      <td class="row">E11-1(E-P)+ pSB1C3 (E-P)</td>
 +
      <td class="row">970<sup>*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
 +
 +
  <tr>
 +
      <td class="row">J101-E5-1C3</td>
 +
      <td class="row">J23101-RBS30-mRFP-TT</td>
 +
      <td class="row">J101-E5 (E-P) + pSB1C3(E-P)</td>
 +
      <td class="row">905*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">J101-31-C3-1</td>
 +
      <td class="row">J23101-RBS31-mRFP-TT</td>
 +
      <td class="row">J101-31 (E-P) + pSB1C3(E-P)</td>
 +
      <td class="row">904*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">04/09/2011</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">J101-E7-1C3-1</td>
 +
      <td class="row">J23101-RBS32-mRFP-TT</td>
 +
      <td class="row">J101-E7 (E-P) + pSB1C3(E-P)</td>
 +
      <td class="row">903*</sup></td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1C3</td>
 +
      <td class="row">Seq OK (BMR Gemonics)</td>
 +
      <td class="row">29/08/2011</td>
 +
  </tr>
 +
 +
</table>
</table>
</center>
</center>
Line 734: Line 954:
<p>
<p>
-
<sup>*</sup> The sequences of these parts contain a 25 bp <a href="http://partsregistry.org/Help:Barcodes">barcode</a>.
+
<div align="center"><sup>*</sup> The sequences of these parts may contain at least a 25 bp <a href="http://partsregistry.org/Help:Barcodes">barcode</a>.</div>

Latest revision as of 13:30, 9 November 2011

UNIPV TEAM 2011

Freezer Management


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.


Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618* E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/2011
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843* E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) / also used as non-fluorescent control in TECAN measurements (RBS) 2009 13 E. coli TOP10 / E. coli MGZ1 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923* E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661* E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/2011
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861* E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/2011
BBa_K3000005 GFP (Silver Standard Prefix) with terminator 2010 854 E. coli TOP10 pSB1C3 Seq ok (BMR Gemonics) FREEZER 2010 Ligations
BBa_K112808 in pSB4C5 Enterobacteria phage T4 Lysis Device - no promoter / also used as non-fluorescent control in TECAN measurements (ENTERO4C5) 2009 1785 E. coli TOP10 / E. coli MGZ1 pSB4C5 Seq ok (BMR Gemonics) FREEZER 2009 Ligations
T9002 BBa_T9002 2009 1945 E. coli TOP10 pSB1A2 Seq ok (MIT) 2009 Registry Distribution
ENTERO-RBS BBa_K112808 in pSB4C5 cotransformed with BBa_B0032 / also used as non-fluorescent control in TECAN measurements 2011 1785 - 13 E. coli TOP10 pSB1A2 - pSB4C5 Seq ok FREEZER 2011 Ligations
T9002-ENTERO BBa_T9002 cotransformed with BBa_K112808 in pSB4C5 / used as measurement device in TECAN measurements 2011 1945 - 1785 E. coli TOP10 pSB1A2 - pSB4C5 Seq ok FREEZER 2011 Ligations


Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 951* E. coli TOP10 pSB1AK3 Length OK 07/07/2011
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 664* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 663* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 662* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969* E. coli TOP10 pSB4C5 Length OK 07/07/2011
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 972* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 971* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 970* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E12-1 RBS34-aiiA-TT E1(X-P)+ B0034(S-P) 969* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 726* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 725* E. coli TOP10 pSB1A2 FAILED
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 724* E. coli TOP10 pSB1A2 FAILED
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 723* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1841* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 926* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
BBa_R0040 in BBa_J61002 pTet in BBa_J61002 plasmid BBa_R0040(S-P)+BBa_J23101(S-P) 923* E. coli TOP10/ E. coli MGZ1 BBa_J61002 Length OK 15/07/2011
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1034* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1033* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1032* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1031* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 726* E. coli MGZ1 pSB4C5 Bad Seq (BMR Genomics) 21/07/2011
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 725* E. coli MGZ1 pSB4C5 FAILED
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 724* E. coli MGZ1 pSB4C5 FAILED
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 723* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 926* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923* E. coli TOP10 pSB4C5 Length ok 25/07/2011
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1034* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1033* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1032* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1031* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E41N-1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 725* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 04/08/2011
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+ E4(X-P) 724* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E43-3 pTet-RBS30-luxI in pSB4C5 E36(S-P)+ E2(X-P) 726* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 25/08/2011
E44-1 pTet-RBS34-mRFP-TT in pSB1C3 BBa_R0040 in BBa_J61002 (E-P) + pSB1C3 (E-P) 923* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 04/09/2011
J101-E5 J23101-RBS30-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E5(S-P) 905* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-31 J23101-RBS31-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E6(S-P) 904* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-E7 J23101-RBS32-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E7(S-P) 903* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-4C5 J23101-RBS34-mRFP-TT in pSB4C5 BBa_J23101(E-P)+ pSB4C5(E-P) 902* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
E3O-1C3-3 RBS31-LuxI E3-1(E-P)+ pSB1C3 (E-P) 663* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 07/09/2011
E4N-1C3-3 RBS32-LuxI E4-2(E-P)+ pSB1C3 (E-P) 662* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 11/09/2011
E5-1C3 RBS30-mRFP-TT E5-2(E-P)+ pSB1C3 (E-P) 864* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E6-1C3 RBS31-mRFP-TT E6-1(E-P)+ pSB1C3 (E-P) 863* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E7-1C3 RBS32-mRFP-TT E7-2(E-P)+ pSB1C3 (E-P) 862* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E9-1C3 RBS30-aiiA-TT E9-2(E-P)+ pSB1C3 (E-P) 972* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E10-1C3 RBS31-aiiA-TT E10-1(E-P)+ pSB1C3 (E-P) 971* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E11-1C3 RBS32-aiiA-TT E11-1(E-P)+ pSB1C3 (E-P) 970* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-E5-1C3 J23101-RBS30-mRFP-TT J101-E5 (E-P) + pSB1C3(E-P) 905* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-31-C3-1 J23101-RBS31-mRFP-TT J101-31 (E-P) + pSB1C3(E-P) 904* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 04/09/2011
J101-E7-1C3-1 J23101-RBS32-mRFP-TT J101-E7 (E-P) + pSB1C3(E-P) 903* E. coli TOP10 pSB1C3 Seq OK (BMR Gemonics) 29/08/2011

* The sequences of these parts may contain at least a 25 bp barcode.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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