Team:Osaka/week3
From 2011.igem.org
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{{Osaka}} | {{Osaka}} | ||
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- | ==August 16(Tue)== | + | ==August 16 (Tue)== |
# Gel electrophoresis of 01,02,03,04,05,06 | # Gel electrophoresis of 01,02,03,04,05,06 | ||
# Transfer to LB culture medium:01,02,03,04,05,06,1-23L | # Transfer to LB culture medium:01,02,03,04,05,06,1-23L | ||
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- | ==August 17(Wed)== | + | ==August 17 (Wed)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
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- | ==August 18(Thu)== | + | ==August 18 (Thu)== |
# Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L | # Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L | ||
# Transformation of 07 and Resistry parts (See Table 3) | # Transformation of 07 and Resistry parts (See Table 3) | ||
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
- | |+ | + | |+Table3 |
!ID!!Part Name!!Resistance!!Description | !ID!!Part Name!!Resistance!!Description | ||
|- | |- | ||
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# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
- | ==August 19(Fri)== | + | ==August 19 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
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- | ==August 20(Sat)== | + | ==August 20 (Sat)== |
# Gel electrophoresis of 1-5A | # Gel electrophoresis of 1-5A | ||
# Gel extraction of 1-5A | # Gel extraction of 1-5A | ||
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# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
+ | |||
+ | |||
+ | |||
+ | [[Team:Osaka/Notebook|Back to Notebook]] | ||
+ | |||
+ | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|'''3'''|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]] |
Latest revision as of 05:53, 19 October 2011
August 16 (Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18 (Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
ID | Part Name | Resistance | Description |
---|---|---|---|
1-6I | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
August 19 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction of 1-1G,1-3A
- Transfer to LB culture medium:1-6I,07
August 20 (Sat)
- Gel electrophoresis of 1-5A
- Gel extraction of 1-5A
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
week 1|2|3|4|5|6|7|8|9|10|11|12|13