"
Thursday, July 21
From 2011.igem.org
(Difference between revisions)
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | ==Tube Prep== | ||
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity. | Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity. | ||
| - | + | ||
| + | These tubes were from the Promoter Plate | ||
*P1 | *P1 | ||
*P2 | *P2 | ||
*P3 | *P3 | ||
*P4 | *P4 | ||
| + | These tubes were from the Terminator Plate | ||
*T1 | *T1 | ||
*T2 | *T2 | ||
*T3 | *T3 | ||
*T4 | *T4 | ||
| + | These tubes were from the Ribosome Binding Site Plate | ||
*R1 | *R1 | ||
*R2 | *R2 | ||
| Line 15: | Line 19: | ||
*R4 | *R4 | ||
| + | ===Incubation=== | ||
| + | The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday. | ||
| + | |||
| + | |||
| + | Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B | ||
| + | |||
| + | :5.0 µl PCR reserved Product | ||
| + | :12.5 µl 10x Buffer | ||
| + | :107.5 µl Nuclease free water | ||
| + | :'''125.0 µl Total''' | ||
| + | --[[User:TravisCR|TravisCR]] 20:27, 21 July 2011 (CDT) | ||
{{LabReportBmore}} | {{LabReportBmore}} | ||
Latest revision as of 01:27, 22 July 2011
Tube Prep
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.
These tubes were from the Promoter Plate
- P1
- P2
- P3
- P4
These tubes were from the Terminator Plate
- T1
- T2
- T3
- T4
These tubes were from the Ribosome Binding Site Plate
- R1
- R2
- R3
- R4
Incubation
The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.
Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B
- 5.0 µl PCR reserved Product
- 12.5 µl 10x Buffer
- 107.5 µl Nuclease free water
- 125.0 µl Total
--TravisCR 20:27, 21 July 2011 (CDT) Team:Baltimore/Notebook
Hours
