Thursday, July 21

From 2011.igem.org

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8 mL of and 8 µL of  
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==Tube Prep==
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Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x.  In addition, each tube contained a single colony.  Each chosen colony was not near any other colonies to preserve diversity.
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These tubes were from the Promoter Plate
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*P1
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*P2
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*P3
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*P4
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These tubes were from the Terminator Plate
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*T1
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*T2
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*T3
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*T4
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These tubes were from the Ribosome Binding Site Plate
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*R1
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*R2
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*R3
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*R4
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===Incubation===
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The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM.  They should be removed in 24 hours, at 4:21 PM Friday.
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Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B
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:5.0  µl PCR reserved Product
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:12.5  µl 10x Buffer
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:107.5 µl Nuclease free water
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:'''125.0 µl Total'''
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--[[User:TravisCR|TravisCR]] 20:27, 21 July 2011 (CDT)
{{LabReportBmore}}
{{LabReportBmore}}

Latest revision as of 01:27, 22 July 2011

Tube Prep

Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.


These tubes were from the Promoter Plate

  • P1
  • P2
  • P3
  • P4

These tubes were from the Terminator Plate

  • T1
  • T2
  • T3
  • T4

These tubes were from the Ribosome Binding Site Plate

  • R1
  • R2
  • R3
  • R4

Incubation

The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.


Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B

5.0 µl PCR reserved Product
12.5 µl 10x Buffer
107.5 µl Nuclease free water
125.0 µl Total

--TravisCR 20:27, 21 July 2011 (CDT) Team:Baltimore/Notebook

Hours

Retrieved from "http://2011.igem.org/Thursday,_July_21"