Team:UNICAMP-EMSE Brazil/Data

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==Data Page==
==Data Page==
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In this section you can find a list of  
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In this section you can find a list of all the parts we made/used in the Stress Wars project. By clicking on each part link you will be redirected to the part page on the Registry of Standard Biological Parts. For more details about the experimental tests, go to our [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Results Results] page.
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This page shows a list of all the parts that we have made or used in the project. Click on the link for each part to see more details about that part on the Registry of Standard Biological Parts. For a brief overview of our main results, please have a look at our Main Results page.
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===How Our System Works===
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===Devices data===
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'''Data For Our Favorite New Parts'''
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554013 Main Page] '''- Protein Secretion system device, BBa_K554013''': This device will be important to export the produced interleukins (IL-12 and IL-10) to the extracellular media. This is a new secretion system device, inspired on Stanford Team 2009. To read a complete description of why BBa_K554013 consists on a new Part, you can click [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#UNICAMP-EMSE_Brazil_Parts_Design:_why_are_they_different_to_Stanford_2009_team_ones.3F HERE] or in the [http://partsregistry.org/Part:BBa_K554013:Experience Experience section of this part]. We could successfuly prove that this part worked as expected through proving the secretion of a reporter gene (GFP) under oxidative stress.<br><br>
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554010 Main Page] '''SoxR device, BBa_K554010''': This part is composed by [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 Constitutive Promoter] linked to [http://partsregistry.org/Part:BBa_K554003 RBS+SoxR transcription factor gene] and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]). We could prove that this part worked through analyzing expression of a reporter gene (GFP) linked to SoxS promoter (which contain SoxR binding site).<br><br>
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554012 Main Page] '''SoxS-RBS-GFP-HlyA device, BBa_K554012''': This part was created to test if SoxS promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554000 BBa_K554000]) and HlyA Secretion signal peptide (http://partsregistry.org/wiki/index.php?title=Part:BBa_K554002 BBa_K554002]) works as expected. We could test and prove GFP production and secretion.<br>
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<font color=red>'''See [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Data#Testing_Devices_.28with_GFP.29 below] the schema containing these three devices in the way they were tested'''</font>
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'''Parts we have improved'''
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#[http://partsregistry.org/Part:BBa_K223062:Experience Experience] '''- Part:BBa_K223062 (Stanford, iGEM 2009)''': We have created a new SoxS linked secretion system inspired in the Stanford 2009 secretion system device and we proved that it worked.<br><br>
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'''Data For Pre-Existing Parts'''
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K223044:Experience Experience] '''- Part:BBa_K223044, anti-inflammatory device (Stanford, iGEM 2009)''':  Although this system has already been designed and used in previously iGEM competitions (iGEM 2009 - https://2009.igem.org/Team:Stanford/ProjectPage), we took advantage of the availability of synthesized sequences to design new parts (SoxR and SoxS) that conforms the common biobrick standards in the iGEM registry. Our team has improved the parts as described in [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#UNICAMP-EMSE_Brazil_Parts_Design:_why_are_they_different_to_Stanford_2009_team_ones.3F Innovation].<br><br>
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'''The following parts were created but we have not tested them yet'''
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We could not create an appropriate test for the following parts:
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554011 Main Page] '''- QseB and QseC device, BBa_K554011''': QseC is the bacterial adrenergic receptor which contains an histidine sensor kinase domain and QseB is its response regulator partner. This device is composed by a constitutive promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], RBS-QseC-RBS-QseB ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554006 BBa_K554006]) and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]).<br><br>
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554014 Main Page] '''- flhDC-IL12-HlyA device, BBa_K554014''': This part contains flhDC promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554001 BBa_K554001]), our fusion construct for IL-12 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554005 BBa_K554005]) linked to HlyA secretion signal peptide ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554002 BBa_K554002] and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011 and http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]).<br><br>
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K554001 Main Page] '''- flhDC promoter, BBa_K554001''': flhDC promoter was designed in to be regulated by Quorum Sensing proteins (QseB and QseC), and thus activate expression of the downstrain linked genes. We also have not already tested the other devices containing this promoter (parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554015 BBa_K554015] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554016 BBa_K554016]).
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'''Some parts are under construction'''
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#'''Nor operon:''' This sequence consists in four genes, norC, norB, norQ and norD, which encode a NO reductase (Nor) complex. This operon can be used to degrade excessive contents of NO due to Th1 overactivation. More details can be found in our [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Linker:_the_Nor_sequence Project] page.
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===Device 1===
 
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===Device 2===
 
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===Device 3===
 
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===Testing Devices (with GFP)===
===Testing Devices (with GFP)===
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We created some devices with replacement of IL-10 and IL-12 by GFP. The following picture represents the systems which we could successfully test, with the following device codes:
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==UNICAMP-EMSE Table of Parts==
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==UNICAMP-EMSE Designed Parts==
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===Parts===
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===Table of Parts===
Here you can find the parts submitted to registry. Each part was extensivelly described, click on the links below and have fun!
Here you can find the parts submitted to registry. Each part was extensivelly described, click on the links below and have fun!

Latest revision as of 00:43, 29 October 2011

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Contents

Data Page

In this section you can find a list of all the parts we made/used in the Stress Wars project. By clicking on each part link you will be redirected to the part page on the Registry of Standard Biological Parts. For more details about the experimental tests, go to our Results page.

How Our System Works


Devices data

Data For Our Favorite New Parts

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554013 Main Page] - Protein Secretion system device, BBa_K554013: This device will be important to export the produced interleukins (IL-12 and IL-10) to the extracellular media. This is a new secretion system device, inspired on Stanford Team 2009. To read a complete description of why BBa_K554013 consists on a new Part, you can click HERE or in the [http://partsregistry.org/Part:BBa_K554013:Experience Experience section of this part]. We could successfuly prove that this part worked as expected through proving the secretion of a reporter gene (GFP) under oxidative stress.

  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554010 Main Page] SoxR device, BBa_K554010: This part is composed by [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 Constitutive Promoter] linked to [http://partsregistry.org/Part:BBa_K554003 RBS+SoxR transcription factor gene] and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]). We could prove that this part worked through analyzing expression of a reporter gene (GFP) linked to SoxS promoter (which contain SoxR binding site).

  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554012 Main Page] SoxS-RBS-GFP-HlyA device, BBa_K554012: This part was created to test if SoxS promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554000 BBa_K554000]) and HlyA Secretion signal peptide (http://partsregistry.org/wiki/index.php?title=Part:BBa_K554002 BBa_K554002]) works as expected. We could test and prove GFP production and secretion.

See below the schema containing these three devices in the way they were tested


Parts we have improved

  1. [http://partsregistry.org/Part:BBa_K223062:Experience Experience] - Part:BBa_K223062 (Stanford, iGEM 2009): We have created a new SoxS linked secretion system inspired in the Stanford 2009 secretion system device and we proved that it worked.

Data For Pre-Existing Parts

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K223044:Experience Experience] - Part:BBa_K223044, anti-inflammatory device (Stanford, iGEM 2009): Although this system has already been designed and used in previously iGEM competitions (iGEM 2009 - https://2009.igem.org/Team:Stanford/ProjectPage), we took advantage of the availability of synthesized sequences to design new parts (SoxR and SoxS) that conforms the common biobrick standards in the iGEM registry. Our team has improved the parts as described in Innovation.

The following parts were created but we have not tested them yet

We could not create an appropriate test for the following parts:

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554011 Main Page] - QseB and QseC device, BBa_K554011: QseC is the bacterial adrenergic receptor which contains an histidine sensor kinase domain and QseB is its response regulator partner. This device is composed by a constitutive promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], RBS-QseC-RBS-QseB ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554006 BBa_K554006]) and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]).

  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554014 Main Page] - flhDC-IL12-HlyA device, BBa_K554014: This part contains flhDC promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554001 BBa_K554001]), our fusion construct for IL-12 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554005 BBa_K554005]) linked to HlyA secretion signal peptide ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554002 BBa_K554002] and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011 and http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]).

  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554001 Main Page] - flhDC promoter, BBa_K554001: flhDC promoter was designed in to be regulated by Quorum Sensing proteins (QseB and QseC), and thus activate expression of the downstrain linked genes. We also have not already tested the other devices containing this promoter (parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554015 BBa_K554015] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554016 BBa_K554016]).


Some parts are under construction

  1. Nor operon: This sequence consists in four genes, norC, norB, norQ and norD, which encode a NO reductase (Nor) complex. This operon can be used to degrade excessive contents of NO due to Th1 overactivation. More details can be found in our Project page.

Testing Devices (with GFP)

We created some devices with replacement of IL-10 and IL-12 by GFP. The following picture represents the systems which we could successfully test, with the following device codes:







UNICAMP-EMSE Designed Parts

Table of Parts

Here you can find the parts submitted to registry. Each part was extensivelly described, click on the links below and have fun!

<groupparts>iGEM011 UNICAMP-EMSE_Brazil</groupparts>


Retrieved from "http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Data"