"
Team:ZJU-China/BBa K561000.html
From 2011.igem.org
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | + | {{Template:Zjucss_modeling}} | |
| + | {{Template:Zjucss_global}} | ||
| + | {{Template:Zjucss_template2}} | ||
<html xmlns="http://www.w3.org/1999/xhtml"> | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
<head> | <head> | ||
| Line 15: | Line 17: | ||
rel='stylesheet' type='text/css'> | rel='stylesheet' type='text/css'> | ||
| - | + | <!-- include the Tools --> | |
| - | + | ||
| - | + | ||
<script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script> | <script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script> | ||
<style type="text/css"> | <style type="text/css"> | ||
| Line 460: | Line 460: | ||
<div id="framecontent"><img | <div id="framecontent"><img | ||
src="https://static.igem.org/mediawiki/2011/9/98/BBa_K561000.jpg" | src="https://static.igem.org/mediawiki/2011/9/98/BBa_K561000.jpg" | ||
| - | style="margin-left: 50px;" /></div> | + | style="margin-left: 50px;" /> |
| + | <a href="http://partsregistry.org/Part:BBa_K561000"> >>go to part registry page</a> | ||
| + | </div> | ||
<div id="importantcontainer"> | <div id="importantcontainer"> | ||
<div class="frame" id="important"> | <div class="frame" id="important"> | ||
Latest revision as of 03:47, 29 October 2011
Biofilm
Rainbofilm
Xfilm
Parts
Achievements
Tools
BBa_K561000
Device
Characterization
The expression of YFP under aerobic conditions
- 1
- 2
The expression of YFP under aerobic conditions is increasing during the exponential phase. With the micro-aerobic promoter, its expression intensity is very low. The highest expression intensity is 1.15. The PoPS of our device is 0.4506.
The expression of YFP under anaerobic conditions
- 1
- 2
Pvgb is a micro-aerobic promoter and it contributes to high expression of YFP under micro-aerobic conditions. It’s expression level at anaerobic condition is relatively low. The PoPS of this device under anaerobic condition is -0.375.
The expression of YFP under micro-aerobic conditions (2.5% oxygen)
- 1
- 2
The expression of YFP with 2.5% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.28. The PoPS of our device at 2.5% oxygen level is 13.9023.
The expression of YFP under micro-aerobic conditions (10%)
- 1
- 2
The expression of YFP with 10% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.24. The PoPS of our device at 10% oxygen level is 2.0653
PoPS of YFP and the expression level of YFP
The YFP expression device has a micro-aerobic promoter, Pvgb. Its PoPS reaches the highest when the oxygen concentration is 2.5%. Under anaerobic or aerobic conditions,its PoPS is equal to 0 relatively.
