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Team:ETH Zurich/xylene
From 2011.igem.org
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| - | 4. For the oil/xylene mixtures we used Raoult's law | + | 4. For the oil/xylene mixtures we assumed that paraffin oil has a vapor pressure of 0 and used Raoult's law |
[[File:Raoult's law.png|600px|left|thumb]] | [[File:Raoult's law.png|600px|left|thumb]] | ||
| + | <br clear="all" /> | ||
| + | {|border="1" align="left" style="text-align:center;" | ||
| + | |xylene in oil in % | ||
| + | |concentration of xylene in LB [mM] | ||
| + | |- | ||
| + | |100 | ||
| + | |3.1 | ||
| + | |- | ||
| + | |50 | ||
| + | |1.55 | ||
| + | |- | ||
| + | |25 | ||
| + | |0.775 | ||
| + | |- | ||
| + | |15 | ||
| + | |0.465 | ||
| + | |- | ||
| + | |10 | ||
| + | |0.31 | ||
| + | |- | ||
| + | |7.5 | ||
| + | |0.233 | ||
| + | |- | ||
| + | |5 | ||
| + | |0.155 | ||
| + | |- | ||
| + | |2.5 | ||
| + | |0.078 | ||
| + | |- | ||
| + | |1 | ||
| + | |0.031 | ||
| + | |- | ||
| + | |0.75 | ||
| + | |0.023 | ||
| + | |- | ||
| + | |0.5 | ||
| + | |0.016 | ||
| + | |- | ||
| + | |0 | ||
| + | |0 | ||
| + | |} | ||
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| + | [[File:ETH Back.png|200px|right|link=Team:ETH_Zurich/Biology/Validation#Results_of_BBa_K625003_characterization]] | ||
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| - | + | = References = | |
| - | <span id="Ref1">[1]Perry’s Chemical Engineers Handbook 8th Edition | + | <span id="Ref1"> [1] Perry’s Chemical Engineers Handbook 8th Edition</span> |
| - | <span id="Ref1">[2] CRC Handbook of Chemistry and Physics, 92nd Edition | + | |
| + | <span id="Ref1">[2] CRC Handbook of Chemistry and Physics, 92nd Edition, p.5-189</span> | ||
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Latest revision as of 21:54, 28 October 2011
Contents |
How we calculated the concentration of m-xylene in the medium...
1. We assumed that 5 ml xylene is enough to saturate the gas phase in the Erlenmeyer flask.
- The vapor pressure was calculated at 37 °C:
- with the following C1-C5 values:
2. The Henry´s constant at 37 °C can be calculated by the Van´t Hoff equation from the literature value at 25°C
3. By Henry´s law we calculated the concentration of xylene in the medium
4. For the oil/xylene mixtures we assumed that paraffin oil has a vapor pressure of 0 and used Raoult's law
| xylene in oil in % | concentration of xylene in LB [mM] |
| 100 | 3.1 |
| 50 | 1.55 |
| 25 | 0.775 |
| 15 | 0.465 |
| 10 | 0.31 |
| 7.5 | 0.233 |
| 5 | 0.155 |
| 2.5 | 0.078 |
| 1 | 0.031 |
| 0.75 | 0.023 |
| 0.5 | 0.016 |
| 0 | 0 |
References
[1] Perry’s Chemical Engineers Handbook 8th Edition
[2] CRC Handbook of Chemistry and Physics, 92nd Edition, p.5-189
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