Team:ZJU-China

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<h1>Project</h1>
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<h1 style="color:red">What's New</h1>
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<hr />
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<hr/>
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<p>&nbsp;&nbsp; Rainbofilm is a stratified expression system in
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<p>
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biofilm, a self-organized module extensible for various needs.
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<strong>Rainbofilm Extension</strong><br/>
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Researchers found a vertical oxygen gradient establishes in the biofilm.
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We extend the current primitive stratification system into a more precise, more controllable, and more versatile device. We start with improving the input signals of the rainbofilm system by Fine-tuning the stratification pattern and Changing the differentiation signal.
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Such property allows us to use oxygen sensitive promoters to
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<a href="https://2011.igem.org/Team:ZJU-China/rainbo-Extension.html">>>go to rainbofilm extension page</a>
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artificially induce differentiated functions through the spatial
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</p>
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distribution of cells. Thus, the multi-step reaction can be processed
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<p><strong>X-film</strong><br/>
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within the different layers of the biofilm.<br />
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After improving the behavior of rainbofilm, we think about more innovative ideas about biofilm. If we could rewire and even fine-tune the input and output, this stratified expression system will act as a machine that receives different inputs and make different responses just like single-input module .Thus we could produce a incredibly robust and engineerable biofilm, X-film.
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&nbsp;&nbsp; The biofilm and its layered structure form spontaneously.
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We then developed X-film into three subprojects:<br/>
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Also biofilm has the natural resistance to high levels of toxin. These
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<strong>Sugarfilm:</strong> Using biofilm to produce fuel.<br/><a href="https://2011.igem.org/Team:ZJU-China/X-Sugarfilm.html">>>go to Sugarfilm page</a><br/>
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two properties render the Rainbofilm a convenient stable system for
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<strong>Sensorfilm:</strong> Using biofilm as a more quantitative and robust biosensor with higher tolerance.<br/><a href="https://2011.igem.org/Team:ZJU-China/X-Sensorfilm.html">>>go to Sensorfilm page</a><br/>
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bio-production and bio-sensor. The system can cater to different needs
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<strong>Gluefilm:</strong> Using biofilm as two-sided tapes that become sticky only after two fitted surface are put together.<br><a href="https://2011.igem.org/Team:ZJU-China/X-Gluefilm.html">>>go to Gluefilm page</a>
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simply by changing downstream genes. One possible application is ethanol
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</p>
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production. The cellulose is degraded to monose from the bottom to the
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<p><strong>Biofilm formation</strong><br/>
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middle layer, and the ethanol is produced and secreted in the surface to
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Quorum sensing plays an important role in biofilm formation. In many Gram-negative bacteria, quorum-sensing systems respond to N-acyl homoserine lactones (AHLs), which were proved to be essential for the architecture of biofilm. Thus we co-transformed our device along with BBa_K091191, a device capable of sending out AI-2 molecules under the control of a pBAD promoter that can be induced with arabinose.<br/><a href="https://2011.igem.org/Team:ZJU-China/bio-results.html" />>>See our results</a></p>
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minimize the toxicity to the inner cells.</p>
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<p><strong>New software tools</strong>
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<ul><li><p><a href="http://zjuchina.tk/rainbosim.php" target="_blank">RainboSim</a> : Design, modeling and share your biofilm. </p></li>
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    <li>
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      <p><a href="http://itunes.apple.com/us/app/zju-china-igem-2011/id476785560?ls=1&mt=8" target="_blank"> ZJU China iGEM 2011 App</a> : A whole view and easy access to ZJU-China's work.</p></li>
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    </ul>
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</p>
</div>
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<div class="links"><a href="#top">Top</a> · <a
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<div class="links"><a href="#top">Top</a> · <a  
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href="https://2011.igem.org/Team:ZJU-China/Project.html">Project page</a></div>
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href="https://2011.igem.org/Team:ZJU-China/X-Overview.html">X film page</a></div>
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<p><a href="https://2011.igem.org/Team:ZJU-China/Safety.html"> Safety
<p><a href="https://2011.igem.org/Team:ZJU-China/Safety.html"> Safety
</a></p>
</a></p>
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</div>
</div>
<div class="links"><a href="#top">Top</a> · <a
<div class="links"><a href="#top">Top</a> · <a
href="https://2011.igem.org/Team:ZJU-China/Humanpractice.html">Human Practice page</a></div>
href="https://2011.igem.org/Team:ZJU-China/Humanpractice.html">Human Practice page</a></div>
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<div class="sidebarbottom"></div>
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</div>
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<div class="block" style="margin-left: 1px; padding-left: 1px"
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id="project">
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<div class="blocktop"></div>
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<div class="blockcontent">
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<h1>Project</h1>
 +
<hr />
 +
<p>Rainbofilm is a stratified expression system in
 +
biofilm, a self-organized module extensible for various needs.
 +
Researchers found a vertical oxygen gradient establishes in the biofilm.
 +
Such property allows us to use oxygen sensitive promoters to
 +
artificially induce differentiated functions through the spatial
 +
distribution of cells. Thus, the multi-step reaction can be processed
 +
within the different layers of the biofilm.<br />
 +
The biofilm and its layered structure form spontaneously.
 +
Also biofilm has the natural resistance to high levels of toxin. These
 +
two properties render the Rainbofilm a convenient stable system for
 +
bio-production and bio-sensor. The system can cater to different needs
 +
simply by changing downstream genes. One possible application is ethanol
 +
production. The cellulose is degraded to monose from the bottom to the
 +
middle layer, and the ethanol is produced and secreted in the surface to
 +
minimize the toxicity to the inner cells.</p>
 +
</div>
 +
<div class="links"><a href="#top">Top</a> · <a href="https://2011.igem.org/Team:ZJU-China/Project.html">Project page</a></div>
 +
<div class="blockbottom"></div>
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<div class="block sidebar" style="margin-right: 1px; padding-right: 1px"
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id="note">
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<div class="sidebartop"></div>
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<div class="blockcontent">
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<h1 style="font-size: 30px;">Achievements</h1>
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<hr />
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<p><strong>We've successfully completed:</strong></p>
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<p>▪ Improving an existing BioBrick Part or Device and enter this information back on the Experience Page of the Registry.</p>
 +
<p>▪ Helping another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</p>
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<p>▪ We've taken a special effort in modeling to make it comprehensive and helpful for each module of our project</p>
 +
<p>▪ We've clearly detailed our approaches to possible biosafety issues related with our project, and answered the safety questions.</p>
 +
</div>
 +
<div class="links"><a href="#top">Top</a> · <a
 +
href="https://2011.igem.org/Team:ZJU-China/judging.html">Achievements</a>·<a href="https://2011.igem.org/Team:ZJU-China/data.html">Data page</a></div>
<div class="sidebarbottom"></div>
<div class="sidebarbottom"></div>
</div>
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Latest revision as of 01:10, 29 October 2011

ZJU-China iGEM 2011

What's New

Rainbofilm Extension
We extend the current primitive stratification system into a more precise, more controllable, and more versatile device. We start with improving the input signals of the rainbofilm system by Fine-tuning the stratification pattern and Changing the differentiation signal. >>go to rainbofilm extension page

X-film
After improving the behavior of rainbofilm, we think about more innovative ideas about biofilm. If we could rewire and even fine-tune the input and output, this stratified expression system will act as a machine that receives different inputs and make different responses just like single-input module .Thus we could produce a incredibly robust and engineerable biofilm, X-film. We then developed X-film into three subprojects:
Sugarfilm: Using biofilm to produce fuel.
>>go to Sugarfilm page
Sensorfilm: Using biofilm as a more quantitative and robust biosensor with higher tolerance.
>>go to Sensorfilm page
Gluefilm: Using biofilm as two-sided tapes that become sticky only after two fitted surface are put together.
>>go to Gluefilm page

Biofilm formation
Quorum sensing plays an important role in biofilm formation. In many Gram-negative bacteria, quorum-sensing systems respond to N-acyl homoserine lactones (AHLs), which were proved to be essential for the architecture of biofilm. Thus we co-transformed our device along with BBa_K091191, a device capable of sending out AI-2 molecules under the control of a pBAD promoter that can be induced with arabinose.
>>See our results

New software tools

Top · X film page

Project

Rainbofilm is a stratified expression system in biofilm, a self-organized module extensible for various needs. Researchers found a vertical oxygen gradient establishes in the biofilm. Such property allows us to use oxygen sensitive promoters to artificially induce differentiated functions through the spatial distribution of cells. Thus, the multi-step reaction can be processed within the different layers of the biofilm.
The biofilm and its layered structure form spontaneously. Also biofilm has the natural resistance to high levels of toxin. These two properties render the Rainbofilm a convenient stable system for bio-production and bio-sensor. The system can cater to different needs simply by changing downstream genes. One possible application is ethanol production. The cellulose is degraded to monose from the bottom to the middle layer, and the ethanol is produced and secreted in the surface to minimize the toxicity to the inner cells.

Top · Project page

Team

We are a young and happy team of 13 scientists and engineers from very diverse backgrounds.Although this is our first year working on a wet lab project and biofilm experiments are new to us, we're currently taking great efforts to build the whole system for experiments.Our team progress and more info will be frequently updated so that our iGEM experience could be shared as much as possible along the way. We truly welcome you to contact us for any cooperation or suggestion.

Top · Team page

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