Latest revision as of 23:59, 28 October 2011

Can you feel the smoke tonight?

SmoColi-Data page

SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works

Our Favorite New Parts

[http://partsregistry.org/Part:BBa_K625000 MainPage]-LacI_M1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI_M1 binds to P_lac and inhibits transcription. (Characterization)
[http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. (Characterization)
[http://partsregistry.org/Part:BBa_K625003 MainPage]-P_U promoter BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)

Characterized New Parts

[http://partsregistry.org/Part:BBa_K625001 MainPage]- P_Tet - LacI_M1 - Termination, BBa_K625001 (Characterization)
[http://partsregistry.org/Part:BBa_K625002 MainPage]-P_U promoter, BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)

Characterized Pre-existing Parts

[http://partsregistry.org/Part:BBa_I13453:Experience Experience]-P_BAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P_BAD in BW27783 (Characterization)

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@@ Line 2: / Line 2: @@
 {{:Team:ETH Zurich/Templates/SectionStart}}
-= Data page =
+= SmoColi-Data page =
-'''SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.'''
+'''SmoColi cells are engineered to sense toxic substances found in cigarette smoke.''' They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
 {{:Team:ETH Zurich/Templates/SectionEnd}}
 {{:Team:ETH Zurich/Templates/SectionStart}}
 = How Our System Works =
-[[File:ETHZ Data page.png|850px|left|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]
+<center>
+<html>
+<img src="/wiki/images/a/ac/ETHZ_Data_page.png" usemap="#Datapage1"/>
+<map name="Datapage1">
+<area shape="rect" alt="" title="" coords="217,122,433,164" href="#Our_Favorite_New_Parts"/>
+<area shape="rect" alt="" title="" coords="217,169,433,186" href="#Characterized New Parts "/>
+<area shape="rect" alt="" title="" coords="0,260,716,296" href="#Our_Favorite_New_Parts"/>
+<area shape="rect" alt="" title="" coords="464,94,532,130" href="#Our_Favorite_New_Parts"/>
+<area shape="rect" alt="" title="" coords="216,192,434,252" href="#Characterized_Pre-existing_Parts"/>
+</map>
+</html>
+</center>
+<!--[[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]-->
 {{:Team:ETH Zurich/Templates/SectionEnd}}
 {{:Team:ETH Zurich/Templates/SectionStart}}
-= Data For Our Favorite New Parts =
-#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]])
+= Our Favorite New Parts =
-#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy number test|Characterization]])
+#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])
-#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor  |Characterization]])
+#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
+#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]])
 {{:Team:ETH Zurich/Templates/SectionEnd}}
 {{:Team:ETH Zurich/Templates/SectionStart}}
-= We've also characterized the following parts =
-#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]])
+=Characterized New Parts =
-#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor|Characterization]])
+#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])
+#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]])
+{{:Team:ETH Zurich/Templates/SectionEnd}}
+{{:Team:ETH Zurich/Templates/SectionStart}}
+=Characterized Pre-existing Parts =
+#[http://partsregistry.org/Part:BBa_I13453:Experience Experience]-'''P<sub>BAD</sub> promoter''', BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P<sub>BAD</sub> in BW27783 ([[Team:ETH_Zurich/Biology/Validation#AraC_-_the_arabinose_sensor|Characterization]])
 {{:Team:ETH Zurich/Templates/SectionEnd}}
 {{:Team:ETH Zurich/Templates/HeaderNewEnd}}

Team:ETH Zurich/Achievements/Data page

From 2011.igem.org