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Team:Osaka/week13
From 2011.igem.org
(Difference between revisions)
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# Gel extraction | # Gel extraction | ||
# Ligation | # Ligation | ||
| - | #* 143(C): | + | #* 143(C):132(upstream)+118(downstream)+1-3A(vector) |
# To liquid culture:131,133,134,142 | # To liquid culture:131,133,134,142 | ||
# Plating (Tolerance assay) | # Plating (Tolerance assay) | ||
| Line 25: | Line 25: | ||
==October 25 (Tue)== | ==October 25 (Tue)== | ||
| - | + | # Miniprep of yesterday's culture | |
| + | # Restriction digests of miniprepped parts | ||
| + | # Electrophoresis of digests | ||
| + | # To liquid culture:130,131,132,133,134,135,136,137,142 | ||
| + | # Colony counts (Tolerance assay) | ||
| + | # Plating (Tolerance assay) | ||
==October 26 (Wed)== | ==October 26 (Wed)== | ||
| - | + | # Miniprep of yesterday's culture | |
| + | # Restriction digests of miniprepped parts | ||
| + | # Electrophoresis of digests | ||
| + | # To liquid culture:131,133,134,137 | ||
| + | # Colony counts (Tolerance assay) | ||
| + | # Plating (Tolerance assay) | ||
==October 27 (Thu)== | ==October 27 (Thu)== | ||
| - | + | # Miniprep of yesterday's culture | |
| + | # Restriction digests of miniprepped parts | ||
| + | # Electrophoresis of digests | ||
| + | # Transformation | ||
| + | # Plating (Tolerance assay) | ||
| + | # Colony counts (Tolerance assay) | ||
==October 28 (Fri)== | ==October 28 (Fri)== | ||
| + | # Colony counts (Tolerance assay) | ||
Latest revision as of 09:47, 28 October 2011
October 23 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- Gel extraction
- Ligation
- 142(C):126(upstream)+118(downstream)+1-3A(vector)
- To liquid culture:130,131,132,133,134,138,139,140,141
- Transformation of ligation products
October 24 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- 131,133,134 x
- Gel extraction
- Ligation
- 143(C):132(upstream)+118(downstream)+1-3A(vector)
- To liquid culture:131,133,134,142
- Plating (Tolerance assay)
- Transformation of ligation products
October 25 (Tue)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- To liquid culture:130,131,132,133,134,135,136,137,142
- Colony counts (Tolerance assay)
- Plating (Tolerance assay)
October 26 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- To liquid culture:131,133,134,137
- Colony counts (Tolerance assay)
- Plating (Tolerance assay)
October 27 (Thu)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- Transformation
- Plating (Tolerance assay)
- Colony counts (Tolerance assay)
October 28 (Fri)
- Colony counts (Tolerance assay)
October 29 (Sat)
week 1|2|3|4|5|6|7|8|9|10|11|12|13
