Team:Osaka/week13

From 2011.igem.org

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# Gel extraction
# Gel extraction
# Ligation
# Ligation
-
#* 142(C):
+
#* 142(C):126(upstream)+118(downstream)+1-3A(vector)
# To liquid culture:130,131,132,133,134,138,139,140,141
# To liquid culture:130,131,132,133,134,138,139,140,141
# Transformation of ligation products
# Transformation of ligation products
==October 24 (Mon)==
==October 24 (Mon)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
#* 131,133,134  x
 +
# Gel extraction
 +
# Ligation
 +
#* 143(C):132(upstream)+118(downstream)+1-3A(vector)
 +
# To liquid culture:131,133,134,142
 +
# Plating (Tolerance assay)
 +
# Transformation of ligation products
==October 25 (Tue)==
==October 25 (Tue)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
# To liquid culture:130,131,132,133,134,135,136,137,142
 +
# Colony counts (Tolerance assay)
 +
# Plating (Tolerance assay)
==October 26 (Wed)==
==October 26 (Wed)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
# To liquid culture:131,133,134,137
 +
# Colony counts (Tolerance assay)
 +
# Plating (Tolerance assay)
==October 27 (Thu)==
==October 27 (Thu)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
# Transformation
 +
# Plating (Tolerance assay)
 +
# Colony counts (Tolerance assay)
==October 28 (Fri)==
==October 28 (Fri)==
 +
# Colony counts (Tolerance assay)

Latest revision as of 09:47, 28 October 2011


October 23 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. Gel extraction
  5. Ligation
    • 142(C):126(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:130,131,132,133,134,138,139,140,141
  7. Transformation of ligation products

October 24 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
    • 131,133,134 x
  4. Gel extraction
  5. Ligation
    • 143(C):132(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:131,133,134,142
  7. Plating (Tolerance assay)
  8. Transformation of ligation products

October 25 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. To liquid culture:130,131,132,133,134,135,136,137,142
  5. Colony counts (Tolerance assay)
  6. Plating (Tolerance assay)

October 26 (Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. To liquid culture:131,133,134,137
  5. Colony counts (Tolerance assay)
  6. Plating (Tolerance assay)

October 27 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. Transformation
  5. Plating (Tolerance assay)
  6. Colony counts (Tolerance assay)

October 28 (Fri)

  1. Colony counts (Tolerance assay)


October 29 (Sat)

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Retrieved from "http://2011.igem.org/Team:Osaka/week13"