Team:ZJU-China/data.html

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         <a href="https://2011.igem.org/Team:ZJU-China/rainbo-modeling-v1.html">Modeling</a>
         <a href="https://2011.igem.org/Team:ZJU-China/rainbo-modeling-v1.html">Modeling</a>
         <a href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a>
         <a href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a>
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<a href="https://2011.igem.org/Team:ZJU-China/rainbo-Extension.html">Extension</a>
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<h4>Sugarfilm</h4>  
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<h4>Xfilm</h4>
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<div class="pane" >  
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<div class="pane"> <a href="https://2011.igem.org/Team:ZJU-China/X-Overview.html">Overview</a> <a href="https://2011.igem.org/Team:ZJU-China/X-Sugarfilm.html">Sugarfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Sensorfilm.html">Sensorfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Gluefilm.html">Gluefilm</a>
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<a href="https://2011.igem.org/Team:ZJU-China/Sugar-background.html">Background</a>
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        <a href="https://2011.igem.org/Team:ZJU-China/Sugar-Design.html">Design</a>
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        <a href="https://2011.igem.org/Team:ZJU-China/Sugar-Modeling.html">Modeling</a>
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              <a href="https://2011.igem.org/Team:ZJU-China/Sugar-Future.html">Future</a>
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   <img src="Zju-glass1.0.gif" width=660;/></p>
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   <img src="http://ung.igem.org/wiki/images/d/d9/Zju-glass1.0.gif" width=660;/></p>
<blockquote>
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   <p id="ref"><strong>Figure 2</strong><br/>
   <p id="ref"><strong>Figure 2</strong><br/>
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  </h1>
  </h1>
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           <div id="banner">
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                 <li>3</li>
                 <li>3</li>
               </ul>
               </ul>
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               <div id="banner_list"> <a href="#" target="_blank"><img src="zju-C(CY)small.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope using a 20X objective, showing the constitutive expressed CFP.
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               <div id="banner_list"> <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/a/a6/Zju-C%28CY%29small.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope using a 20X objective, showing the constitutive expressed CFP.
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" /></a> <a href="#" target="_blank"><img src="zju-Y(CY)short.png
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" /></a> <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/a/a6/Zju-C%28CY%29small.png
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" title="" alt="Growth Rate under aerobic conditions" /></a> <a href="#" target="_blank"><img src="zju-CYmerge(CY)short.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) and YFP(micro-aerobic condition induced) under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective. " /></a> </div>
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" title="" alt="Growth Rate under aerobic conditions" /></a> <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/e/e1/Zju-CYmerge%28CY%29short.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) and YFP(micro-aerobic condition induced) under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective. " /></a> </div>
             </div>
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               <p style="text-align:center"><embed src="CY.mov"  autostart="true" loop="true" width="520" height="400"/></embed></p>
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               <p style="text-align:center"><embed src="http://ung.igem.org/wiki/images/1/18/CY.mov"  autostart="true" loop="true" width="520" height="400"/></embed></p>
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               <p style="text-align:center"> <img src="ZJU-CY.gif" width="520" height="400"></p>
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               <p style="text-align:center"> <img src="http://ung.igem.org/wiki/images/a/a1/ZJU-CY.gif" width="520" height="400"></p>
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     </ul>
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   <div id="banner_list1">
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         <a href="#" target="_blank"><img src="zju-C(RC)long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective, showing the constitutive expressed CFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/d/d3/Zju-C%28RC%29long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective, showing the constitutive expressed CFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-R(RC)long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing  the anaerobically induced RFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/9/93/Zju-R%28RC%29long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing  the anaerobically induced RFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-merged(RC)long.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) and RFP(anaerobic condition induced) under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/2/2f/Zju-merged%28RC%29long.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) and RFP(anaerobic condition induced) under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective.  
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   <embed src="RC.mov" autostart="true" loop="true"  width="520" height="400"/>   
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   <embed src="http://ung.igem.org/wiki/images/8/8b/CR.mov" autostart="true" loop="true"  width="520" height="400"/>   
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<img src="zju-RC.gif" width="600" height="400"></p>
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<img src="http://ung.igem.org/wiki/images/8/85/Zju-RC.gif" width="600" height="400"></p>
                
                
  <h1>Rainbofilm (Yellow &amp; Red) </h1>
  <h1>Rainbofilm (Yellow &amp; Red) </h1>
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     </ul>
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   <div id="banner_list2">
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         <a href="#" target="_blank"><img src="zju-R(RY)short.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective, showing  the anaerobically induced RFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/3/35/Zju-R%28RY%29short.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective, showing  the anaerobically induced RFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-Y(RY)short.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope using a 20X objective, showing the micro-aerobic condition induced YFP.
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/7/76/Zju-Y%28RY%29short.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM 710NLO confocal laser scanning microscope using a 20X objective, showing the micro-aerobic condition induced YFP.
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-RYmerge(RY)short.png" title="" alt="Merged fluorescent biofilm picture of RFP (anaerobic condition induced) and YFP(micro-aerobic condition induced) under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/a/a7/Zju-RYmerge%28RY%29short.png" title="" alt="Merged fluorescent biofilm picture of RFP (anaerobic condition induced) and YFP(micro-aerobic condition induced) under Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective.  
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<embed src="YR.mov" autostart="true" loop="true" width="520" height="400"/></embed></p>
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<embed src="http://ung.igem.org/wiki/images/e/e2/YR.mov" autostart="true" loop="true" width="520" height="400"/></embed></p>
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<p style="text-align:center"><img src="zju-RY.gif" width="561" height="420"></p>
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<p style="text-align:center"><img src="http://ung.igem.org/wiki/images/b/bd/Zju-RY.gif" width="561" height="420"></p>
  <h1>Rainbofilm (Rainbow) </h1>
  <h1>Rainbofilm (Rainbow) </h1>
  <p>&nbsp;</p>
  <p>&nbsp;</p>
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     </ul>
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   <div id="banner_list3">
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         <a href="#" target="_blank"><img src="zju-C(CYR)long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective, showing the constitutive expressed CFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/e/e8/Zju-C%28CYR%29long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective, showing the constitutive expressed CFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-R(CYR)long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing  the anaerobically induced RFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/8/85/Zju-R%28CYR%29long.png" title="" alt="Fluorescent image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing  the anaerobically induced RFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-YCYR)long.png" title="" alt="Fluorescence image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing micro-aerobic condition induced YFP.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/8/8e/Zju-YCYR%29long.png" title="" alt="Fluorescence image of the biofilm under Zeiss LSM510 Meta confocal laser scanning microscope using a 63X oil-immersion objective, showing micro-aerobic condition induced YFP.  
" /></a>
" /></a>
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         <a href="#" target="_blank"><img src="zju-merge(CYR)long.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) YFP (micro-aerobic condition induced) and RFP (anaerobic condition induced) under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective.  
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         <a href="#" target="_blank"><img src="http://ung.igem.org/wiki/images/d/dc/Zju-merge%28CYR%29long.png" title="" alt="Merged fluorescent biofilm picture of CFP (aerobic condition induced) YFP (micro-aerobic condition induced) and RFP (anaerobic condition induced) under Zeiss LSM510 Meta confocal laser scanning microscope, using a 63X oil-immersion objective.  
" /></a>
" /></a>
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     </div>
</div>
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<embed src="CYR.mov" autostart="true" loop="true" width="520" height="400"/></p>
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<embed src="http://ung.igem.org/wiki/images/b/b9/CYR.mov" autostart="true" loop="true" width="520" height="400"/></p>
  </div>
  </div>
   
   

Latest revision as of 18:35, 28 October 2011

Data

Biofilm

Background Design Modeling Results

Rainbofilm

Background Design Modeling Results Extension

Xfilm

Overview SugarfilmSensorfilmGluefilm

Parts

Parts-BBa_I13602 Parts-BBa_K561000 Parts-BBa_K561002 Parts-BBa_K561003

Achievements

Data Page Judging Form Attribution and Contributions

Tools

Tools

Data Page

Experiment Results

Biofilm Formation

 

Figure 1
The figure shows the modified DH5alpha's biofilm formed on rubber tube. The pink color was due to the RFP expressed by the bacteria. The picture was obtained with Leica DME microscope, under 40X objective.

Figure 2
3D CLSM picture of the biofilm formed by E. Coli DH5alpha. The picture was obtained with Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective. The colors of the biofilm were not from the fluorescence proteins but artificially added in order to give better resolution.

Figure 3
CLSM image of the stratified biofilm obtained with the Cell Culture Plate Method. It was formed by our modified E.Coli expressing device BBa_K561003 (you can refer to our Rainbofilm module for more information). The picture was taken by Zeiss LSM510 Meta confocal laser scanning microscope, using a 63? oil-immersion objective.

Rainbofilm (Blue & Yellow)

 


Rainbofilm (Blue & Red)

 

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Rainbofilm (Yellow & Red)

 

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Rainbofilm (Rainbow)

 

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Parts Characterization

BBa_I13602

The expression of CFP under aerobic and anaerobic conditions

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Fluorescence intensity of CFP under anaerobic conditions Growth Rate under anaerobic conditions Fluorescence intensity of CFP under aerobic conditions Growth Rate under aerobic conditions

 

BBa_K561000

The expression of YFP under aerobic conditions

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Fluorescenc intensity of YFP under aerobic conditions Growth Rate under aerobic conditions

The expression of YFP under anaerobic conditions

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BBa_K561002

The expression of RFP under aerobic conditions

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The expression of RFP under anaerobic conditions

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The expression of RFP under micro-aerobic conditions (2.5%)

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PoPS of RFP and the expression level of RFP

 

BBa_K561003

The expression of CFP repressed by tetR which is expressed by subpart of BBa_K561000 or BBa_K561002:

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