Team:ZJU-China/BBa K561002.html

From 2011.igem.org

(Difference between revisions)
(Created page with "<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta...")
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
+
{{Template:Zjucss_modeling}}
 +
{{Template:Zjucss_global}}
 +
{{Template:Zjucss_template2}}
<html xmlns="http://www.w3.org/1999/xhtml">
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
Line 15: Line 17:
rel='stylesheet' type='text/css'>
rel='stylesheet' type='text/css'>
-
<link href="http://zjuchina.tk/modeling.css" rel="stylesheet" type="text/css">
+
<!-- include the Tools -->
-
<link href="http://zjuchina.tk/global.css" rel="stylesheet" type="text/css">
+
-
<link href="http://zjuchina.tk/template2.css" rel="stylesheet" type="text/css"><!-- include the Tools -->
+
<script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script>
<script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script>
<style type="text/css">
<style type="text/css">
Line 378: Line 378:
<a href="https://2011.igem.org/Team:ZJU-China/rainbo-Design.html">Design</a> <a
<a href="https://2011.igem.org/Team:ZJU-China/rainbo-Design.html">Design</a> <a
href="https://2011.igem.org/Team:ZJU-China/rainbo-modeling-v1.html">Modeling</a> <a
href="https://2011.igem.org/Team:ZJU-China/rainbo-modeling-v1.html">Modeling</a> <a
-
href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a></div>
+
href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a><a href="https://2011.igem.org/Team:ZJU-China/rainbo-Extension.html">Extension</a></div>
-
<h4>Sugarfilm</h4>
+
<h4>Xfilm</h4>
-
<div class="pane"><a
+
<div class="pane"><a href="https://2011.igem.org/Team:ZJU-China/X-Overview.html">Overview</a> <a href="https://2011.igem.org/Team:ZJU-China/X-Sugarfilm.html">Sugarfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Sensorfilm.html">Sensorfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Gluefilm.html">Gluefilm</a></div>
-
href="https://2011.igem.org/Team:ZJU-China/Sugar-background.html">Background</a> <a
+
-
href="https://2011.igem.org/Team:ZJU-China/Sugar-Design.html">Design</a> <a
+
-
href="https://2011.igem.org/Team:ZJU-China/Sugar-Modeling.html">Modeling</a> <a
+
-
href="https://2011.igem.org/Team:ZJU-China/Sugar-Future.html">Future</a></div>
+
<h4 class="current">Parts</h4>
<h4 class="current">Parts</h4>
Line 441: Line 437:
<p><img src="https://static.igem.org/mediawiki/2011/6/68/BBa_K561002.jpg"
<p><img src="https://static.igem.org/mediawiki/2011/6/68/BBa_K561002.jpg"
style="margin-left: 50px;" /></p>
style="margin-left: 50px;" /></p>
 +
<a href="http://partsregistry.org/Part:BBa_K561002"> >>go to part registry page</a>
<div id="importantcontainer">
<div id="importantcontainer">
<div class="frame" id="important">
<div class="frame" id="important">

Latest revision as of 03:48, 29 October 2011

Parts-BBa_K561002

Biofilm

Rainbofilm

Xfilm

Parts

Achievements

Tools

BBa_K561002

Device

>>go to part registry page

Characterization

The expression of RFP under aerobic conditions

The expression of RFP under aerobic conditions is increasing during the exponential phase. With the anaerobic promoter, its expression intensity is very low. The highest expression intensity is 1.12. The PoPS of our device is 0.3065.

 

The expression of RFP under anaerobic conditions

  • 1
  • 2

  The device of RFP has an anaerobic promoter, which leads to its high expression under anaerobic conditions, as it shows in the above graph. It reveals that its highest expression level is 1.12. The PoPS of our device is 10.45.

 

The expression of RFP under micro-aerobic conditions (2.5%)

  • 1
  • 2
  • 3
  • 4

  The anaerobic promoter (PfdhF) has low expression under micro-aerobic conditions. At 2.5% oxygen and 10% oxygen, its expression level when reaching the exponential phase is 1.02 and 1.05 respectively. The PoPS of the device at 2.5% oxygen and 10% oxygen are 0.4122 and 0.2127, respectively.

 

PoPS of RFP and the expression level of RFP

The RFP expression device has an anaerobic promoter, PfdhF. Its PoPS reaches the highest under anaerobic conditions. Under micro-aerobic or aerobic conditions,its PoPS is equal to 0 relatively.