Team:Tokyo Metropolitan/Notebook/S05

From 2011.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 4: Line 4:
</html>
</html>
-
='''-Molecular Genetics Microbiology-'''=  
+
='''-Speeding up-'''=  
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Line 13: Line 13:
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
 +
 +
 +
= -Visualization- =
 +
-Ligation
 +
 +
J23100-I714051
 +
 +
 +
 +
-Transformation
 +
 +
J23100-I714051
 +
 +
 +
-Plasmid extraction
 +
 +
Promoter and suppressed expression system
 +
 +
 +
-DNA refining
 +
 +
Promoter and suppressed expression system

Latest revision as of 13:20, 5 October 2011

-Speeding up-

Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.

Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.

Amplified and purification I20260 with PCR.

Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI


-Visualization-

-Ligation

J23100-I714051


-Transformation

J23100-I714051


-Plasmid extraction

Promoter and suppressed expression system


-DNA refining

Promoter and suppressed expression system