"
Team:Tokyo Metropolitan/Notebook/S05
From 2011.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Tokyo_Metropolitan/Header}} | {{:Team:Tokyo_Metropolitan/Header}} | ||
| + | <html> | ||
| + | <div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> | ||
| + | </html> | ||
| + | |||
| + | ='''-Speeding up-'''= | ||
| - | |||
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | ||
| Line 9: | Line 13: | ||
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | ||
| + | |||
| + | |||
| + | = -Visualization- = | ||
| + | -Ligation | ||
| + | |||
| + | J23100-I714051 | ||
| + | |||
| + | |||
| + | |||
| + | -Transformation | ||
| + | |||
| + | J23100-I714051 | ||
| + | |||
| + | |||
| + | -Plasmid extraction | ||
| + | |||
| + | Promoter and suppressed expression system | ||
| + | |||
| + | |||
| + | -DNA refining | ||
| + | |||
| + | Promoter and suppressed expression system | ||
Latest revision as of 13:20, 5 October 2011
 |
 |
 |
 |
 |
 |
 |
 |
-Speeding up-
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
-Visualization-
-Ligation
J23100-I714051
-Transformation
J23100-I714051
-Plasmid extraction
Promoter and suppressed expression system
-DNA refining
Promoter and suppressed expression system
