Team:Tokyo Metropolitan/Notebook/S05

From 2011.igem.org

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Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
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='''-Speeding up-'''=
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Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
 +
 
 +
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
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 +
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= -Visualization- =
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-Ligation
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J23100-I714051
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-Transformation
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J23100-I714051
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 +
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-Plasmid extraction
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 +
Promoter and suppressed expression system
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 +
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-DNA refining
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 +
Promoter and suppressed expression system

Latest revision as of 13:20, 5 October 2011

-Speeding up-

Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.

Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.

Amplified and purification I20260 with PCR.

Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI


-Visualization-

-Ligation

J23100-I714051


-Transformation

J23100-I714051


-Plasmid extraction

Promoter and suppressed expression system


-DNA refining

Promoter and suppressed expression system

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S05"