Team:KAIT Japan/Project

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!align="center"|[[Team:KAIT_Japan|Home]]
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!align="center"|[[Team:KAIT_Japan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=KAIT_Japan Official Team Profile]
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!align="center"|[[Team:KAIT_Japan/Project|Project]]
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!align="center"|[[Team:KAIT_Japan/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:KAIT_Japan/Modeling|Modeling]]
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!align="center"|[[Team:KAIT_Japan/Notebook|Notebook]]
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!align="center"|[[Team:KAIT_Japan/Attributions|Attributions]]
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<title>無題 2</title>
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== '''Colony on Colony''' ==
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&nbsp;&nbsp;&nbsp;  We tried to overlap colony on colony. Generally, colony and colony don’t overlap and approach each other, because of the cell-cell communications. Bacterias also have a system, so called quorum sensing, to control of production of signal chemicals depend on the bacteria’s density.
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&nbsp;&nbsp;&nbsp; We pay attention quorum sensing system. Bacterias produce and secret autoinducer against other bacterias by quorum sensing system, as a result of the system, bacteria senses other bacterias. Most of Gram-negative bacterias make use of autoinducer named AHL. We applied E. coli to our experiment and we use aiia which is catabolic enzyme to inhibit AHL. We tried to make a recombinant bacteria to cancelled sensing of other bacterias and expected to form the colony of bacterias on the other bacterias.
 
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<h2><span id="Project" class="style1">Project</span></h2>
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<p class="style2"><strong>Back Ground</strong></p>
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<p class="style3">Bacteria detect the concentration of its friends,
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called Quorum Sensing, and the sensing system is one of the communication tools
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between microbes. It is related also to formation of biofilm and to
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pathogenicity. <br>As the Quorum Sensing of Gram-negative bacterias,
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Acyl-Homoserine Lactone <br>(AHL ) is released to recognize each other. 2007,
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the team of Chiba University made the vacteria production of aiia to decrease
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the AHL.<br>We considered application of the system to make a colony layer on a
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colony layer by<br>depressing of Quorum Sensing.</p>
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<p class="style2"><strong>Purpose</strong></p>
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<p class="style3">We make bacteria that have transgene of AiiA, and get
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the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and
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General, colony and colony don’t approach by AHL.<br>Our projects were done to
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approach and overlap.</p>
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<p class="style3">&nbsp;</p>
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<p class="style3">&nbsp;</p>
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<p class="style4"><span id="result_box" class="short_text" lang="en"><span class="hps">
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<h2 class="style1"><span id="Word" class="style1">Word</span></h2>
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<p class="style5"><strong>Quorum Sensing</strong></p>
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<p class="style3">Quorum sensing is a system of stimulus and response
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correlated to population density. <br>Many species of bacteria use quorum
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sensing to coordinate gene expression according to the density of their local
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population. In similar fashion, some social insects use quorum sensing to
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determine where to nest.</p>
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<p class="style5"><strong>AHL and AiiA</strong></p>
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<p class="style3">AHL is [N-] acyl-homoserine lactones. <br>AHL is a
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small transmitter that is used in quorum sensing system autoinducer (AI) is
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known as. AHL is present in many gram-negative bacteria<br>In low cell density,
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AHL becomes low concentration, and AHL becomes high concentration in high cell
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density. <br>At high cell densities, the AIs can accumulate to a concentration
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sufficient for activation of related regulatory genes. Because the concentration
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of AHL&#39;s is a key factor in determining virulence gene expression in several
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pathogenic bacteria, it is possible to develop a strategy for disease control by
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controlling production of AIs or eliminating AIs produced by pathogenic
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bacteria.<br><br>AiiA, an enzyme that inactivates the acylhomoserine lactone
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quorum-sensing signal.</p>
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<p class="style5"><strong>Autoinducer </strong></p>
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<p class="style3">Autoinducers are chemical signaling molecules that are
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produced and used by bacteria participating in quorum sensing. </p>
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 02:19, 6 October 2011

無題 2

Home Team Official Project Notebook Safety Sponsors


無題 2

Project

Back Ground

Bacteria detect the concentration of its friends, called Quorum Sensing, and the sensing system is one of the communication tools between microbes. It is related also to formation of biofilm and to pathogenicity.
As the Quorum Sensing of Gram-negative bacterias, Acyl-Homoserine Lactone
(AHL ) is released to recognize each other. 2007, the team of Chiba University made the vacteria production of aiia to decrease the AHL.
We considered application of the system to make a colony layer on a colony layer by
depressing of Quorum Sensing.

Purpose

We make bacteria that have transgene of AiiA, and get the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and General, colony and colony don’t approach by AHL.
Our projects were done to approach and overlap.

 

 

Word

Quorum Sensing

Quorum sensing is a system of stimulus and response correlated to population density.
Many species of bacteria use quorum sensing to coordinate gene expression according to the density of their local population. In similar fashion, some social insects use quorum sensing to determine where to nest.

AHL and AiiA

AHL is [N-] acyl-homoserine lactones.
AHL is a small transmitter that is used in quorum sensing system autoinducer (AI) is known as. AHL is present in many gram-negative bacteria
In low cell density, AHL becomes low concentration, and AHL becomes high concentration in high cell density.
At high cell densities, the AIs can accumulate to a concentration sufficient for activation of related regulatory genes. Because the concentration of AHL's is a key factor in determining virulence gene expression in several pathogenic bacteria, it is possible to develop a strategy for disease control by controlling production of AIs or eliminating AIs produced by pathogenic bacteria.

AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal.

Autoinducer

Autoinducers are chemical signaling molecules that are produced and used by bacteria participating in quorum sensing.

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