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-	We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis.	+	==22 Septembre 2011==
-	We use the PCR protocol that functions well with our gene (PCR Protocol) and we test all primers because we don’t know which primers the team used the last year for genes amplification.	+	'''We did:'''
		+
		+	1. Broth of XL10 gold (50 microliters in 2 ml of LB).
		+
		+	2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.

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Latest revision as of 04:18, 29 September 2011

22 Septembre 2011

We did:

1. Broth of XL10 gold (50 microliters in 2 ml of LB).

2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.