Team:Fatih Turkey/Results
From 2011.igem.org
(Difference between revisions)
Buraksimsek (Talk | contribs) |
|||
(5 intermediate revisions not shown) | |||
Line 561: | Line 561: | ||
<!-- post container --> | <!-- post container --> | ||
<div> | <div> | ||
- | + | <div class="meta-left-full"> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
Line 614: | Line 567: | ||
<p> | <p> | ||
- | <p><strong><em></em><em>For our project lab executions;</em></strong></p> | + | </p><p><strong><em></em><em>For our project lab executions;</em></strong></p> |
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p> | <p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p> | ||
Line 620: | Line 573: | ||
B.subtilis one for E.coli work expectedly. | B.subtilis one for E.coli work expectedly. | ||
- | <em> </em></p> | + | <em> </em></em></p><em> |
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen | <p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen | ||
- | working well.<em> </em></p> | + | working well.<em> </em></em></p><em> |
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not | <p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not | ||
Line 631: | Line 584: | ||
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis- | Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis- | ||
- | E.coli mixed culture. <em> </em></p> | + | E.coli mixed culture. <em> </em></em></p><em> |
<p>DISC EXPERIMENT1</p> | <p>DISC EXPERIMENT1</p> | ||
Line 673: | Line 626: | ||
children. | children. | ||
</p> | </p> | ||
- | <p><strong><br | + | <p><strong><br> |
</strong></p> | </strong></p> | ||
Line 742: | Line 695: | ||
<h2>THE SUICIDE EXPERIMENT OF E. COLI</h2> | <h2>THE SUICIDE EXPERIMENT OF E. COLI</h2> | ||
<p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p> | <p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p> | ||
- | <p>< | + | <p><img src="https://static.igem.org/mediawiki/igem.org/6/6e/S1.png"></p> |
- | <p>< | + | <p><img src="https://static.igem.org/mediawiki/2011/4/40/S2.png"></p> |
<p>After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup>, 4<sup>th</sup> plates OD values didn’t change. But the the OD value of 5<sup>th</sup> plate increased. 5<sup>th</sup> plate is the control group.</p> | <p>After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup>, 4<sup>th</sup> plates OD values didn’t change. But the the OD value of 5<sup>th</sup> plate increased. 5<sup>th</sup> plate is the control group.</p> | ||
- | <p>< | + | <p><img src="https://static.igem.org/mediawiki/2011/0/02/DSC03586_-_Kopya.JPG"></p> |
<p> </p> | <p> </p> | ||
<p>OFR is more effective than DFR for B,subtilis.</p> | <p>OFR is more effective than DFR for B,subtilis.</p> | ||
Line 752: | Line 705: | ||
- | </div> | + | </em></em></em></div> |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 08:22, 5 October 2011
2011 © Fatih Medical School