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Team:ETH Zurich/Biology
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| - | {{:Team:ETH Zurich/Templates/ | + | {{:Team:ETH Zurich/Templates/HeaderNew}} |
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| - | {| | + | {{:Team:ETH Zurich/Templates/SectionStart}} |
| - | + | = Biology - Overview = | |
| + | '''A brief overview of our SmoColi system can be found here, click on the experiments or subparts to jump to the corresponding sections.''' | ||
| + | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
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| + | {{:Team:ETH Zurich/Templates/SlideShow|Items=<html> | ||
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| + | <div><img src="/wiki/images/d/d9/ETH-SLIDESHOW_BIOLOGY-1.PNG"/></div> | ||
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| + | <div><img src="/wiki/images/2/21/ETH-SLIDESHOW_BIOLOGY1.PNG" usemap="#BIOSlide1"/> | ||
| + | <map name="BIOSlide1"> | ||
| + | <area shape="rect" alt="" title="" coords="182,86,436,128" href="/Team:ETH_Zurich/Biology/Detector#Acetaldehyde_Sensor"/> | ||
| + | <area shape="rect" alt="" title="" coords="188,144,442,186" href="/Team:ETH_Zurich/Biology/Detector#Xylene_Sensor" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="188,196,442,238" href="/Team:ETH_Zurich/Biology/Detector#Arabinose_Sensor" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="72,244,638,338" href="/Team:ETH_Zurich/Biology/Detector#Bandpass" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="72,342,444,436" href="/Team:ETH_Zurich/Biology/Detector#Quorum_Sensing_of_the_Alarm_system" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="458,92,626,132" href="/Team:ETH_Zurich/Biology/Validation#AlcR-_the_acetaldehyde_sensor" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="456,148,624,188" href="/Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="454,198,622,238" href="/Team:ETH_Zurich/Biology/Validation#AraC_-_the_arabinose_sensor" target="" /> | ||
| + | <area shape="rect" alt="" title="" coords="456,348,624,390" href="/Team:ETH_Zurich/Biology/Validation#Alarm" /> | ||
| + | <area shape="rect" alt="" title="" coords="638,24,748,446" href="/Team:ETH_Zurich/Biology/Validation" target="" /> | ||
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| + | </map> | ||
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| + | </div> | ||
| + | <div><img src="/wiki/images/8/8d/ETH-SLIDESHOW_BIOLOGY2.PNG" usemap="#BIOSlide2" /> | ||
| + | <map name="BIOSlide2"> | ||
| + | <area shape="rect" alt="" title="" coords="38,124,494,412" href="/Team:ETH_Zurich/Biology/MolecularMechanism#Plasmid_design" /> | ||
| + | <area shape="rect" alt="" title="" coords="502,142,740,378" href="/Team:ETH_Zurich/Biology/MolecularMechanism#Definition_of_ribosome_binding_sites" target="" /> | ||
| + | </map> | ||
| + | </div> </html> | ||
| + | }} | ||
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| + | <!--n | ||
[[File:ETH SmoColi.png|200px|left|thumb|'''Overview of the SmoColi system.''' Depending on the concentration of smoke respectively sensor molecule, a GFP band appears at a certain distance from the entry of the tube. Beyond a certain concentration cells turn red.]] | [[File:ETH SmoColi.png|200px|left|thumb|'''Overview of the SmoColi system.''' Depending on the concentration of smoke respectively sensor molecule, a GFP band appears at a certain distance from the entry of the tube. Beyond a certain concentration cells turn red.]] | ||
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== BioBricks and experimental results == | == BioBricks and experimental results == | ||
| - | Our BioBricks added to the registry can be found in the [[Team:ETH Zurich/Achievements/BioBricks|BioBrick]] chapter. | + | Our BioBricks added to the registry can be found in the [[Team:ETH Zurich/Achievements/BioBricks|BioBrick]] chapter. We characterized the parts BBa_K625000 and BBa_K625001 and added the results to the [[Team:ETH Zurich/Achievements/Results|experimental results]]. For other experiments performed and background information of experiments swith to the [[Team:ETH Zurich/Biology/Validation|Validation]] chapter. |
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{|class="roundContainer" | {|class="roundContainer" | ||
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| + | [[File:SmoColi.JPG|350px|right|thumb|'''SmoColi pixels''' | ||
| + | :)As a side project we sorted and spotted ''E. coli'' expressing either RFP, GFP or β-galactosidase with a FACS machine.]] | ||
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== Network elements == | == Network elements == | ||
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3. alarm system | 3. alarm system | ||
| - | Both a repressor (coupled to an inverter) or an activator can be used as sensor parts of the system. In our case we used AlcR (from '' | + | Both a repressor (coupled to an inverter) or an activator can be used as sensor parts of the system. In our case we used AlcR (from ''Aspergillus nidulans''), respectively XylR (from ''Pseudomonas putida'') regulatory proteins in order to sense compounds present in smoke and thereby to induce the different parts of our network. |
Upon sensing the specific molecule the circuit gets activated and our SmoColi bacteria start notifying us about the presence of toxic substances in air. For a detailed description of the circuit design please [https://2011.igem.org/Team:ETH_Zurich/Biology/Detector click here]. | Upon sensing the specific molecule the circuit gets activated and our SmoColi bacteria start notifying us about the presence of toxic substances in air. For a detailed description of the circuit design please [https://2011.igem.org/Team:ETH_Zurich/Biology/Detector click here]. | ||
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Latest revision as of 20:50, 28 October 2011
Biology - Overview
A brief overview of our SmoColi system can be found here, click on the experiments or subparts to jump to the corresponding sections.
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