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| <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div> | | <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div> |
| <ul> | | <ul> |
- | <li class="toclevel-1"><a href="#Autoclave"><span class="tocnumber">1</span> <span class="toctext">Autoclave</span></a></li> | + | <li class="toclevel-1"><a href="#TECAN_Infinite_2000"><span class="tocnumber">1</span> <span class="toctext">TECAN Infinite 2000</span></a></li> |
- | <li class="toclevel-1"><a href="#Incubator_For_Plates"><span class="tocnumber">2</span> <span class="toctext">Incubator for plates</span></a></li> | + | <li class="toclevel-1"><a href="#Nanodrop_ND_1000_spectrophotometer"><span class="tocnumber">2</span> <span class="toctext">Nanodrop ND 1000 spectrophotometer</span></a></li> |
- | <li class="toclevel-1"><a href="#Incubator_And_Shaker_For_Liquid_Cultures"><span class="tocnumber">3</span> <span class="toctext">Incubator and shaker for liquid cultures</span></a></li> | + | <li class="toclevel-1"><a href="#PCR_thermocyclers"><span class="tocnumber">3</span> <span class="toctext">PCR thermocyclers</span></a></li> |
- | <li class="toclevel-1"><a href="#Hood"><span class="tocnumber">4</span> <span class="toctext">Hood</span></a></li> | + | <li class="toclevel-1"><a href="#Instruments_For_Electrophoresis"><span class="tocnumber">4</span> <span class="toctext">Instruments for electrophoresis</span></a></li> |
- | <li class="toclevel-1"><a href="#Water_Bath"><span class="tocnumber">5</span> <span class="toctext">Water bath</span></a></li>
| + | <li class="toclevel-1"><a href="#Gel_Image_Acquisition_System"><span class="tocnumber">5</span> <span class="toctext">Gel image acquisition system</span></a></li> |
- | <li class="toclevel-1"><a href="#Instruments_For_Electrophoresis"><span class="tocnumber">6</span> <span class="toctext">Instruments for electrophoresis</span></a></li>
| + | <li class="toclevel-1"><a href="#Incubator_For_Plates"><span class="tocnumber">6</span> <span class="toctext">Incubator for plates</span></a></li> |
- | <li class="toclevel-1"><a href="#Gel_Image_Acquisition_System"><span class="tocnumber">7</span> <span class="toctext">Gel image acquisition system</span></a></li> | + | <li class="toclevel-1"><a href="#Incubator_And_Shaker_For_Liquid_Cultures"><span class="tocnumber">7</span> <span class="toctext">Incubator and shaker for liquid cultures</span></a></li> |
- | <li class="toclevel-1"><a href="#Cold_Instruments"><span class="tocnumber">8</span> <span class="toctext">Cold instruments</span></a></li> | + | <li class="toclevel-1"><a href="#Hood"><span class="tocnumber">8</span> <span class="toctext">Laminar flow cabinet</span></a></li> |
- | <li class="toclevel-1"><a href="#Nanodrop_ND_1000_spectrophotometer"><span class="tocnumber">9</span> <span class="toctext">Nanodrop ND 1000 spectrophotometer</span></a></li> | + | <li class="toclevel-1"><a href="#Autoclave"><span class="tocnumber">9</span> <span class="toctext">Autoclave</span></a></li> |
- | <li class="toclevel-1"><a href="#TECAN_Infinite_2000"><span class="tocnumber">10</span> <span class="toctext">TECAN Infinite 2000</span></a></li> | + | |
- | <li class="toclevel-1"><a href="#Bacteria_Room_And_Molecular_Biology_Room"><span class="tocnumber">11</span> <span class="toctext">Bacteria room and Molecular Biology room</span></a></li> | + | |
| </ul></td> | | </ul></td> |
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| </tbody> | | </tbody> |
| </table> | | </table> |
- | <script type="text/javascript"> if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script> | + | <script type="text/javascript"> if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script> <br> |
| <a name="TECAN_Infinite_2000"></a> | | <a name="TECAN_Infinite_2000"></a> |
| <h2> <span class="mw-headline">TECAN Infinite 2000</span></h2> | | <h2> <span class="mw-headline">TECAN Infinite 2000</span></h2> |
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| This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. | | This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. |
| We used this instrument for the following measurement techniques: </p> | | We used this instrument for the following measurement techniques: </p> |
- | <p></p> | + | <div class="listcircle"> |
- | <ul>
| + | <div style="text-align:justify"> |
- | <li> fluorescence intensity; </li>
| + | <div class="art-postcontent" style="margin-left: 15pt;"> |
| + | <li> fluorescence intensity; </li> |
| <li> absorbance. </li> | | <li> absorbance. </li> |
- | </ul></td> | + | </div></div></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="TECAN Infinite 2000" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_tecan.jpg/250px-UNIPV_Pavia_tecan.jpg" border="0" height="198" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="TECAN Infinite 2000" src="https://static.igem.org/mediawiki/2010/5/5f/UNIPV_Pavia_tecan.jpg" border="0" height="198" width="250"></span></div></td> |
| + | </tr> |
| + | <tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>TECAN infinite 2000</span></div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| <a name="Nanodrop_ND_1000_spectrophotometer"></a> | | <a name="Nanodrop_ND_1000_spectrophotometer"></a> |
- | <h2> <span class="mw-headline">Nanodrop ND 1000 spectrophotometer</span></h2> | + | <h2> <span class="mw-headline">NanoDrop ND 1000 spectrophotometer</span></h2> |
| <table width="100%"> | | <table width="100%"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
| <td align="left" valign="top"><p></p> | | <td align="left" valign="top"><p></p> |
- | <p align="justify"> Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample and OD in cell cultures.</p> | + | <p align="justify"> The Thermo Scientific NanoDrop™ 1000 Spectrophotometer (v 3.7) is a full-spectrum (220 - 750nm) spectrophotometer. This instrument is a really helpfull instrument that elimante the needs of cuvettes and is capable to measure highly concentrated samples without dilution. We use NanoDrop to measure: |
- | <p></p></td> | + | <div class="listcircle"> |
| + | <div style="text-align:justify"> |
| + | <div class="art-postcontent" style="margin-left: 15pt;"> |
| + | <div class="listcircle"> |
| + | <li> concentration of DNA in a micro-volume of sample (1 microliter);</li> |
| + | <li> and OD in cell cultures (2 microliter).</li></p> |
| + | <p></p></div></div></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Nanodrop" src="https://static.igem.org/mediawiki/2010/thumb/2/21/UNIPV_Pavia_nanodrop.gif/150px-UNIPV_Pavia_nanodrop.gif" border="0" height="147" width="150"></span></div></td> | + | <td><div class="floatright"><span><img alt="Nanodrop" src="https://static.igem.org/mediawiki/2011/3/36/Nanodrop_ND1000.jpg" border="0" width="250"></span></div></td> |
| + | </tr> |
| + | <tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>NanoDrop ND 1000</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| </p> | | </p> |
| | | |
- | <a name="PCR thermocyclers"></a> | + | <a name="PCR_thermocyclers"></a> |
| <h2> <span class="mw-headline">PCR thermocyclers</span></h2> | | <h2> <span class="mw-headline">PCR thermocyclers</span></h2> |
| <table width="100%"> | | <table width="100%"> |
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| <tr> | | <tr> |
| <td align="left" valign="top"><p></p> | | <td align="left" valign="top"><p></p> |
- | <p align="justify"> AAAAAAAAAAAAAAAAAAAAAAAAAAA </p> | + | <p align="justify"> The laboratory is equipped with: |
| + | <div class="listcircle"> |
| + | <div style="text-align:justify"> |
| + | <div class="art-postcontent" style="margin-left: 15pt;"> |
| + | <li>Gene Amp PCR System 9700 (Applied Biosystem) thermal cycler: used to amplify segments of DNA via the polymerase chain reaction (PCR) process; </li> |
| + | <li>MiniOpticon Real-Time PCR Instrument (BIO-RAD): this machine combines the functions of a thermal cycler and a fluorimeter. It is able to amplify and detect DNA.</li></p> |
| <p></p></td> | | <p></p></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
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| <tr> | | <tr> |
| <td><div class="floatright"><span><img alt="Real time" src="https://static.igem.org/mediawiki/2008/d/dd/Pv_pcr_parallel2.jpg" border="0" height="188" width="250"></span></div></td> | | <td><div class="floatright"><span><img alt="Real time" src="https://static.igem.org/mediawiki/2008/d/dd/Pv_pcr_parallel2.jpg" border="0" height="188" width="250"></span></div></td> |
| + | </tr> |
| + | <tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>MiniOpticon Real-Time PCR</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| <tr> | | <tr> |
| <td><div class="floatright"><span><img alt="thermocycler" src="https://static.igem.org/mediawiki/2008/0/08/Pv_pcr_parallel1.jpg" border="0" height="188" width="250"></span></div></td> | | <td><div class="floatright"><span><img alt="thermocycler" src="https://static.igem.org/mediawiki/2008/0/08/Pv_pcr_parallel1.jpg" border="0" height="188" width="250"></span></div></td> |
| + | </tr> |
| + | </tr> |
| + | <tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>Gene Amp PCR System 9700</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Instruments for electrophoresis" src="https://static.igem.org/mediawiki/2010/thumb/3/31/UNIPV_Pavia_electrophoresis.jpg/250px-UNIPV_Pavia_electrophoresis.jpg" border="0" height="188" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Instruments for electrophoresis" src="https://static.igem.org/mediawiki/2010/3/31/UNIPV_Pavia_electrophoresis.jpg" border="0" height="188" width="250"></span></div></td> |
| + | </tr> |
| + | <tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>Large, medium and small electrophoresis apparatuses</span></div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| <tr> | | <tr> |
| <td align="left" valign="top"><p></p> | | <td align="left" valign="top"><p></p> |
- | <p align="justify"> It is a UV system with camera used to acquire gel run snapshots. </p> | + | <p align="justify"> Transilluminator projects ultraviolet radiation for the observation of agarose gel ethidium bromide-stained. |
| + | Also gel acquisition image position is equipped with a digital camera used to acquire gel run snapshots. </p> |
| <p></p></td> | | <p></p></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Gel image acquisition system" src="https://static.igem.org/mediawiki/2010/thumb/3/34/UNIPV_Pavia_gelacq.jpg/250px-UNIPV_Pavia_gelacq.jpg" border="0" height="188" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Gel image acquisition system" src="https://static.igem.org/mediawiki/2011/b/bb/Gelacquisition.jpg" border="0" height="188" width="250"></span></div></td> |
| </tr> | | </tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>UV system and camera</span> </div></td> |
| + | </tr> |
| + | |
| </tbody> | | </tbody> |
| </table></td> | | </table></td> |
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| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Incubator for plates" src="https://static.igem.org/mediawiki/2010/thumb/0/0a/UNIPV_Pavia_incubatorforplates.jpg/250px-UNIPV_Pavia_incubatorforplates.jpg" border="0" height="188" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Incubator for plates" src="https://static.igem.org/mediawiki/2010/0/0a/UNIPV_Pavia_incubatorforplates.jpg" border="0" height="188" width="250"></span></div></td> |
| </tr> | | </tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>Incubator for plates</span> </div></td> |
| + | </tr> |
| + | |
| </tbody> | | </tbody> |
| </table></td> | | </table></td> |
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| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Incubator and shaker for liquid cultures" src="https://static.igem.org/mediawiki/2010/thumb/5/5f/UNIPV_Pavia_incubatorshaker.jpg/250px-UNIPV_Pavia_incubatorshaker.jpg" border="0" height="188" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Incubator and shaker for liquid cultures" src="https://static.igem.org/mediawiki/2010/5/5f/UNIPV_Pavia_incubatorshaker.jpg" border="0" height="188" width="250"></span></div></td> |
| + | </tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>Incubator and shaker for liquid cultures</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| <a name="Hood"></a> | | <a name="Hood"></a> |
- | <h2> <span class="mw-headline">Hood</span></h2> | + | <h2> <span class="mw-headline">Laminar flow cabinet</span></h2> |
| <table width="100%"> | | <table width="100%"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
| <td align="left" valign="top"><p></p> | | <td align="left" valign="top"><p></p> |
- | <p align="justify"> It is a laminar flow hood for handling highly toxic products. </p> | + | <p align="justify"> The lab is equipped with laminar flow cabinet. It is a carefully enclosed bench designed to prevent contamination of biological samples. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. The hood is provided of UV-C germicidal lamp for the sterilization. </p> |
| <p></p></td> | | <p></p></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Hood" src="https://static.igem.org/mediawiki/2010/thumb/d/d4/UNIPV_Pavia_hood.jpg/250px-UNIPV_Pavia_hood.jpg" border="0" height="238" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Hood" src="https://static.igem.org/mediawiki/2010/d/d4/UNIPV_Pavia_hood.jpg" border="0" height="238" width="250"></span></div></td> |
| + | </tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>Laminar flow cabinet</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| </p> | | </p> |
| | | |
- | <!-----centrifughe>
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- | </a><a name="Autoclave"></a>
| + | <a name="Autoclave"></a> |
| <h2> <span class="mw-headline">Autoclave</span></h2> | | <h2> <span class="mw-headline">Autoclave</span></h2> |
| <table width="100%"> | | <table width="100%"> |
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| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td><div class="floatright"><span><img alt="Autoclave" src="https://static.igem.org/mediawiki/2010/thumb/7/7d/UNIPV_Pavia_autoclave.jpg/250px-UNIPV_Pavia_autoclave.jpg" border="0" height="188" width="250"></span></div></td> | + | <td><div class="floatright"><span><img alt="Autoclave" src="https://static.igem.org/mediawiki/2010/7/7d/UNIPV_Pavia_autoclave.jpg" border="0" height="188" width="250"></span></div></td> |
| + | </tr> |
| + | <td><div style='text-align:center; font-size: 12px; font-style:italic;'><span>The autoclave</span> </div></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| + | {{end}} |