Team:Washington/Protocols

From 2011.igem.org

(Difference between revisions)
(Make It: Magnetosome Protocols)
 
(29 intermediate revisions not shown)
Line 1: Line 1:
{{Template:Team:Washington/Templates/Top}}
{{Template:Team:Washington/Templates/Top}}
__NOTOC__
__NOTOC__
 +
 +
<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br>
=General Protocols=
=General Protocols=
Line 18: Line 20:
[https://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
-
[https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagensis]
+
[https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]
-
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
+
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
Line 36: Line 38:
[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
 +
 +
[https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning]
 +
 +
[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team]
=Break It:  Gluten Destruction Protocols=
=Break It:  Gluten Destruction Protocols=
-
[https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Cell Lysate Assay]
+
[https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay]
[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
 +
[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay]
-
=Make It: Magnetosome Protocols=
+
 
 +
=Make It: iGEM Toolkits=
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)]
[https://2011.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)]
Line 52: Line 62:
[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
 +
 +
[https://2011.igem.org/Team:Washington/Protocols/High_PCR High-Yield PCR (Full-Gene Assembly)]
[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)]
[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)]
-
[https://2011.igem.org/Team:Washington/Protocols/Micros. Microscopy Protocol]
+
[https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
 +
 
 +
 
=Wiki Design=
=Wiki Design=
-
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools]
+
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)]

Latest revision as of 02:10, 29 September 2011


Protocols


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagenesis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Alkane Biosynthesis cloning

Cell Lysate Assay by Decarbonylase Redesign Team


Break It: Gluten Destruction Protocols

Whole Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Enzyme Assay


Make It: iGEM Toolkits

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Induction Studies of Proteins Fusions (mam-sfGFP)

pGA Vector Assay

PBS Stock Protocol

Preparation of Overnight Cultures


Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)