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SEPTEMBER: WEEK 1
September, 2ndE3-1C3-2 was purified in order to screen the part length.
R0040 in J61002-1C3, E3N-1C3 and J101-31N-1C3 were transformed in 200 μl of TOP10 competent cells.
September, 3rd
All the cultures were diluted 1:200 in M9 with the proper antibiotic. After two hours T9002-ENTERO and ENTERO-RBS were aliquoted in 96-well microplate and induced with known concentrations of 3OC6-HSL and with the supernatants collected on August, 31st.
 Small size gel
The sample did not show the correct band.
September, 4thR0040 in J61002-1C3-1, R0040 in J61002-1C3-2, J101-31-1C3-1 and J101-31-1C3-2 were glycerol stocked and then plasmidic DNA was purified with MiniPrep commercial kit:
2.5 μl of each sample were digested with 0.5 μl EcoRI and PstI resctriction endonucleases in a 25 μl final volume. A small size agarose gel was prepared. In the afternoon electrophoresis was carried out; all samples showed the correct insert.  Small size gel
Inoculum of T9002-ENTERO and ENTERO-RBS to measure 3OC6-HSL concentration in supernatants collected on August, 31st.
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Team:UNIPV-Pavia/Calendar/September/week1
From 2011.igem.org
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<a name="September.2C_2nd"></a><h2> <span class="mw-headline">September, 2nd</span></h2> | <a name="September.2C_2nd"></a><h2> <span class="mw-headline">September, 2nd</span></h2> | ||
<p> | <p> | ||
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| + | <div class="center"><div class="thumb tnone"><div class="thumbinner" style="width: 550px;"><a href="/File:UNIPV_03_09_2011_E3-1C3_2_EP.PNG" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/2/20/UNIPV_03_09_2011_E3-1C3_2_EP.PNG" class="thumbimage" height="50%" width="50%"></a> <div class="thumbcaption">Small size gel</div></div></div></div> | ||
<p> | <p> | ||
| - | The | + | The sample did not show the correct band. |
<br> | <br> | ||
500 ml M9 and 1 l LB were prepared. | 500 ml M9 and 1 l LB were prepared. | ||
</p> | </p> | ||
| + | |||
<div align="right"><small><a href="#indice" title="">^top</a></small></div> | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
| + | <a name="September.2C_4th"></a><h2> <span class="mw-headline">September, 4th</span></h2> | ||
| + | <p> | ||
| + | R0040 in J61002-1C3-1, R0040 in J61002-1C3-2, J101-31-1C3-1 and J101-31-1C3-2 were glycerol stocked and then plasmidic DNA was purified with MiniPrep commercial kit: | ||
| + | </p> | ||
| + | <center> | ||
| + | <table class="data"> | ||
| + | <tr> | ||
| + | <td class="row"><b>Plasmid</b></td> | ||
| + | <td class="row"><b>DNA (ng/μl)</b></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td class="row">R0040 in J61002-1C3-1</td> | ||
| + | <td class="row">66.2</td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | <tr> | ||
| + | <td class="row">R0040 in J61002-1C3-2</td> | ||
| + | <td class="row">81.4</td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | <tr> | ||
| + | <td class="row">J101-31-1C3-1</td> | ||
| + | <td class="row">86.9</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td class="row">J101-31-1C3-2</td> | ||
| + | <td class="row">98.3</td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | </center> | ||
| + | |||
| + | <p> | ||
| + | 2.5 μl of each sample were digested with 0.5 μl EcoRI and PstI resctriction endonucleases in a 25 μl final volume. A small size agarose gel was prepared. In the afternoon electrophoresis was carried out; all samples showed the correct insert. | ||
| + | </p> | ||
| + | |||
| + | <div class="center"><div class="thumb tnone"><div class="thumbinner" style="width: 550px;"><a href="/File:UNIPV_04_09_2011_R1_R2_J10131_1_J10131_2_EP.PNG" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/f/f4/UNIPV_04_09_2011_R1_R2_J10131_1_J10131_2_EP.PNG" class="thumbimage" height="50%" width="50%"></a> <div class="thumbcaption">Small size gel</div></div></div></div> | ||
| + | |||
| + | <p> | ||
| + | Inoculum of T9002-ENTERO and ENTERO-RBS to measure 3OC<small><sub>6</sub></small>-HSL concentration in supernatants collected on <a href="https://2011.igem.org/Team:UNIPV-Pavia/Calendar/August/week5#August.2C_31st">August, 31st</a>. | ||
| + | <br> | ||
| + | Inoculum of E31-1, E41N-1, E42-1, E43-2, E43-3 and ENTERO-4C5 to collect the supernatants and measure the 3OC<small><sub>6</sub></small>-HSL produced by luxI enzyme. | ||
| + | </p> | ||
| + | |||
| + | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
| + | |||
| + | <br> | ||
<div> | <div> | ||
<span style="float:left;"> | <span style="float:left;"> | ||
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</span> | </span> | ||
</div> | </div> | ||
| - | + | </div> | |
</html> | </html> | ||
{{endcalendar}} | {{endcalendar}} | ||
Latest revision as of 10:16, 18 September 2011
