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AUGUST: WEEK 5
August, 29th
T9002-ENTERo and ENTERO-RBS were diluted 1:500 in M9 + Amp + Cm12.5 13 ml and 1 ml volume respectively. After six hour growth they were aliquoted in 96-well microplate (190 μl cultures) with 10 μl of supernatants collected on August, 27th and 10 μl of 3OC6-HSL.
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Team:UNIPV-Pavia/Calendar/August/week5
From 2011.igem.org
(Difference between revisions)
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<p><a name="indice"/> | <p><a name="indice"/> | ||
</p> | </p> | ||
| - | <a name="August. | + | <a name="August.2C_29th"></a><h2> <span class="mw-headline">August, 29th</span></h2> |
| - | + | <p> | |
| - | + | T9002-ENTERo and ENTERO-RBS were diluted 1:500 in M9 + Amp + Cm12.5 13 ml and 1 ml volume respectively. After six hour growth they were aliquoted in 96-well microplate (190 μl cultures) with 10 μl of supernatants collected on <a name="August.2C_27th">August, 27th</a> and 10 μl of 3OC<sub><small>6</small></sub>-HSL. | |
| - | + | <br> | |
| - | + | 5 flasks were prepared with 29.6 ml H<sub><small>2</small></sub>O, 200 μl glycerol 80 %, 8 ml M9 salts 5 x and their pH was adjusted to 5.9, 6.3, 6.7, 6.9 and 7.2; they were autoclaved in order to prepare M9 glycerol supplemented minimal medium. | |
| - | + | <br> | |
| + | E43-3 DNA was digested with Ecori and PstI restriction nucleases in order to screen the part length by gel-electrophoresis; it showed the correct band. | ||
| + | </p> | ||
<div align="right"><small><a href="#indice" title="">^top</a></small></div> | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
Revision as of 21:11, 1 September 2011
