Team:UNIPV-Pavia/Calendar/August/week4

From 2011.igem.org

(Difference between revisions)
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<a name="August.2C_22nd"></a><h2> <span class="mw-headline">August, 22nd</span></h2>
<a name="August.2C_22nd"></a><h2> <span class="mw-headline">August, 22nd</span></h2>
<p>
<p>
-
Streak of E17-2, E18-2, E19-2, E20-2, J101-31, J101-4C5, ENTERO-4C5 on LB agar + Cm12.5 plate to test Plux promoter with different RBSs.
+
Streak of E17-2, E18-2, J101-31, J101-E5 and ENTERO-4C5 on LB agar + Cm12.5 plate to test Plux promoter with different RBSs.
<br>
<br>
E24-1, E25-2, E26-1, E27-2 and E41N-1 purified DNA samples were sent to BMR Genomics for sequencing.
E24-1, E25-2, E26-1, E27-2 and E41N-1 purified DNA samples were sent to BMR Genomics for sequencing.
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</p>
</p>
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<div align="right"><small><a href="#indice" title="">^top</a></small></div>
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<a name="August.2C_23rd"></a><h2> <span class="mw-headline">August, 23rd</span></h2>
 +
<p>
 +
T9002-ENTERO and ENTERO-RBS were diluted 1:500 in a final volume of 1 ml of M9 + Cm12.5.
 +
<br>
 +
Three colonies of E17-2, E18-2, J101-31, J101-E5 and ENTERO-4C5 were picked and inoculated in 1 ml of M9 + Cm12.5.
 +
<br>
 +
E19-2, E 20-2, J101-E7, J101-4C5 and ENTEOR-4C5 were streaked on a LB agar + Cm12.5 plate.
 +
<br>
 +
Unfortunately TECAN test could not be performed as the instrument did not work!
 +
<br>
 +
31 LB agar + Cm34 plates were prepared.
 +
<br>
 +
Last week sequencing were ready: E28-1 has not the desired sequence, while E37-2, E38-1, E39-1, E40-2 and E42-1 were correct.
 +
<br>
 +
Ptet-RBS30-luxI ligation was done again from E36 and E2:
 +
</p>
 +
<center>
 +
<table class="data">
 +
    <tr>
 +
      <td class="row"><b>Ligation Name</b></td>
 +
      <td class="row"><b>Vector</b></td>
 +
      <td class="row"><b>Vector volume (&mu;l)</b></td>
 +
      <td class="row"><b>Insert</b></td>
 +
      <td class="row"><b>Insert volume (&mu;l)</b></td>
 +
      <td class="row"><b>Buffer (&mu;l)</b></td>
 +
      <td class="row"><b>T4 Ligase (&mu;l)</b></td>
 +
  </tr>
 +
    <tr>
 +
      <td class="row"><b>E43</b></td>
 +
      <td class="row">E36 (S-P)</td>
 +
      <td class="row">3.5</td>
 +
      <td class="row">E2 (X-P)</td>
 +
      <td class="row">4.5</td>
 +
      <td class="row">1</td>
 +
      <td class="row">1</td>
 +
  </tr>
-
 
+
</table>
-
 
+
</center>

Revision as of 21:37, 30 August 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

AUGUST: WEEK 4

August, 22nd

Streak of E17-2, E18-2, J101-31, J101-E5 and ENTERO-4C5 on LB agar + Cm12.5 plate to test Plux promoter with different RBSs.
E24-1, E25-2, E26-1, E27-2 and E41N-1 purified DNA samples were sent to BMR Genomics for sequencing.
250 ml of M9 glycerol supplemented minimal medium were prepared.
Inoculum of T9002-ENTERO and ENTERO-RBS to test supernatants collected on August, 20th to test AiiA enzyme efficiency.

August, 23rd

T9002-ENTERO and ENTERO-RBS were diluted 1:500 in a final volume of 1 ml of M9 + Cm12.5.
Three colonies of E17-2, E18-2, J101-31, J101-E5 and ENTERO-4C5 were picked and inoculated in 1 ml of M9 + Cm12.5.
E19-2, E 20-2, J101-E7, J101-4C5 and ENTEOR-4C5 were streaked on a LB agar + Cm12.5 plate.
Unfortunately TECAN test could not be performed as the instrument did not work!
31 LB agar + Cm34 plates were prepared.
Last week sequencing were ready: E28-1 has not the desired sequence, while E37-2, E38-1, E39-1, E40-2 and E42-1 were correct.
Ptet-RBS30-luxI ligation was done again from E36 and E2:

Ligation Name Vector Vector volume (μl) Insert Insert volume (μl) Buffer (μl) T4 Ligase (μl)
E43 E36 (S-P) 3.5 E2 (X-P) 4.5 1 1

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