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Team:UNICAMP-EMSE Brazil/Notebook/21 August 2011
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==21 August 2011== | ==21 August 2011== | ||
===Everybody:=== | ===Everybody:=== | ||
| - | + | '''Objective:''' Check the digestion of plasmids bearing the promotors (yesterday we could not see if it worked appropriately because the gel concentration was too low to distinguish the small bands (i.e. promoter bands)). | |
| - | '''Objective:''' Check the digestion of plasmids bearing the promotors (yesterday we could not see if it | + | |
*Agarose: 1,5% (We increased the gel concentration today); | *Agarose: 1,5% (We increased the gel concentration today); | ||
*Ladder: 100 bp Bio-Rad | *Ladder: 100 bp Bio-Rad | ||
| - | <br> | + | <br>[[Image:UNICAMP-EMSE_Brazil_Gel_21_08_11.png|thumb|right|300px|alt=Gel 1: flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)| Electrophoresis Gel, 21/08/2011.]] |
| + | |||
*'''Picture legend:''' | *'''Picture legend:''' | ||
#'''Gel:''' flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d) | #'''Gel:''' flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d) | ||
| Line 19: | Line 19: | ||
:*''Constitutive'' – 35 bp | :*''Constitutive'' – 35 bp | ||
:*''Constitutive + mRFP'' – around 700 bp | :*''Constitutive + mRFP'' – around 700 bp | ||
| + | <br> | ||
| + | *'''See below the representation of the plasmid [http://partsregistry.org/Part:BBa_J23119 BBa_J23119] containing mRFP:''' <br> | ||
| + | <br>[[Image:PLASMID_BBa_J23119.png|center|link=http://partsregistry.org/Part:BBa_J23119]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <html> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <script language='JavaScript' type='text/JavaScript'> | ||
| + | |||
| + | function makeQuote() { | ||
| + | |||
| + | Q = new Array(); | ||
| + | |||
| + | |||
| + | Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)" | ||
| + | Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga" | ||
| + | Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor" | ||
| + | Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student" | ||
| + | Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair" | ||
| + | Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil" | ||
| + | Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress" | ||
| + | Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)" | ||
| + | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
| + | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
| + | |||
| + | |||
| + | |||
| + | |||
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| + | |||
| + | |||
| + | |||
| + | |||
| + | index = Math.floor(Math.random() * Q.length); | ||
| + | |||
| + | document.writeln(Q[index]); | ||
| + | |||
| + | } | ||
| + | |||
| + | </script> | ||
| + | |||
| + | </head> | ||
| + | |||
| + | <style> | ||
| + | #newquote {color:gray; font-family:verdana; font-size:11px; text-align:right; padding:5px;} | ||
| + | |||
| + | #newquote i {display:block; color:black; font-family:georgia, trebuchet ms; font-size:13px;} | ||
| + | |||
| + | #newquote small {display:block; text-align:right; margin-top:10px;} | ||
| + | |||
| + | ]]></b:skin> | ||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <div id='newquote'> | ||
| + | |||
| + | <script language="javascript"> | ||
| + | |||
| + | makeQuote(); | ||
| + | |||
| + | </script> | ||
| + | </div> | ||
| + | </head> | ||
| + | </html> | ||
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21 August 2011
Everybody:
Objective: Check the digestion of plasmids bearing the promotors (yesterday we could not see if it worked appropriately because the gel concentration was too low to distinguish the small bands (i.e. promoter bands)).
- Agarose: 1,5% (We increased the gel concentration today);
- Ladder: 100 bp Bio-Rad
- Picture legend:
- Gel: flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)
Nd: non digested
D: digested
D: digested
- Fragment lengths:
- flhDC- 126 bp
- SoxS – 103 bp
- Constitutive – 35 bp
- Constitutive + mRFP – around 700 bp
- See below the representation of the plasmid [http://partsregistry.org/Part:BBa_J23119 BBa_J23119] containing mRFP:
