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Team:UNICAMP-EMSE Brazil/Notebook/19 August 2011
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==19 August 2011== | ==19 August 2011== | ||
===Synthetic DNA sequences status=== | ===Synthetic DNA sequences status=== | ||
| + | [[Image:UNICAMP-EMSE_Brazil_Gel1_19_08_11.png|thumb|right|450px|<font color: blue>'''Electrophoresis Gel 1, 19/08/2011.''']] | ||
'''Objective:''' Check the size of synthetic DNA sequences. | '''Objective:''' Check the size of synthetic DNA sequences. | ||
*'''Ladder:''' 100-10.000 bp (Fermentas) | *'''Ladder:''' 100-10.000 bp (Fermentas) | ||
*'''Agarose concentration:''' 0.8% | *'''Agarose concentration:''' 0.8% | ||
*'''Samples: ''' | *'''Samples: ''' | ||
| - | |||
| - | |||
:*'''GEL 1:''' | :*'''GEL 1:''' | ||
::*1. Const. Promoter1 (nd) | ::*1. Const. Promoter1 (nd) | ||
| Line 26: | Line 25: | ||
::*15. RBS-IL12 2 | ::*15. RBS-IL12 2 | ||
::*16. Ladder | ::*16. Ladder | ||
| - | [[Image:UNICAMP-EMSE_Brazil_Gel2_19_08_11.png|thumb|right| | + | [[Image:UNICAMP-EMSE_Brazil_Gel2_19_08_11.png|thumb|right|450px|<font color: blue>'''Electrophoresis Gel 2, 19/08/2011.''']] |
:*'''GEL2:''' | :*'''GEL2:''' | ||
| Line 39: | Line 38: | ||
::*25. RBS-HylD 1 | ::*25. RBS-HylD 1 | ||
::*26. RBS-HylD 2 | ::*26. RBS-HylD 2 | ||
| + | <br clear="all" /> | ||
| + | *'''<font color=teal>Results:'''</font> | ||
| + | <font align=center> | ||
| + | {|border="1" cellpadding="5" cellspacing="0" align="center" | ||
| + | |- | ||
| + | ! scope="col" style="background:#efefef;" | Gene | ||
| + | ! scope="col" style="background:#efefef;" | Total size (gene+vector) | ||
| + | ! scope="col" style="background:#efefef;" | Linear vector size | ||
| + | ! scope="col" style="background:#efefef;" | Gene size | ||
| + | ! scope="col" style="background:#efefef;" | Result | ||
| + | |- | ||
| + | |'''Constitut. promoter''' | ||
| + | |2795 | ||
| + | |2760 | ||
| + | |35 | ||
| + | |Vector size Ok. No promoter band visible. | ||
| + | |- | ||
| + | |'''flhDC promoter''' | ||
| + | |2926 | ||
| + | |2800 | ||
| + | |126 | ||
| + | |Vector size Ok. No promoter band visible. Parcial digestion? | ||
| + | |- | ||
| + | |'''RBS+HlyD''' | ||
| + | |4296 | ||
| + | |2800 | ||
| + | |1496 | ||
| + | |Fragment size Ok. Parcial digestion. | ||
| + | |- | ||
| + | |'''RBS-IL10''' | ||
| + | |3345 | ||
| + | |2800 | ||
| + | |545 | ||
| + | |Fragment size Ok. Parcial digestion. | ||
| + | |- | ||
| + | |'''RBS-IL12''' | ||
| + | |4458 | ||
| + | |2800 | ||
| + | |1658 | ||
| + | |Fragment size Ok. Parcial digestion. | ||
| + | |- | ||
| + | |'''RBS+HlyB''' | ||
| + | |4983 | ||
| + | |2800 | ||
| + | |2183 | ||
| + | |Fragment size Ok. Parcial digestion, where´s linear vector? | ||
| + | |- | ||
| + | |'''RBS+HlyA''' | ||
| + | |3027 | ||
| + | |2800 | ||
| + | |227 | ||
| + | |Fragment size Ok. Parcial digestion. | ||
| + | |- | ||
| + | |'''RBS+SoxR''' | ||
| + | |3323 | ||
| + | |2800 | ||
| + | |523 | ||
| + | |Fragment size Ok. Parcial digestion. | ||
| + | |- | ||
| + | |'''RBS+QseB+RBS+QseC''' | ||
| + | |4886 | ||
| + | |2800 | ||
| + | |2086 | ||
| + | |Fragment size Ok. Parcial digestion, where´s linear vector? | ||
| + | |- | ||
| + | |}</font> | ||
| + | <br> | ||
| + | <font color=teal>'''- Repeat digestions with less DNA.'''</font> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <script language='JavaScript' type='text/JavaScript'> | ||
| + | |||
| + | function makeQuote() { | ||
| + | |||
| + | Q = new Array(); | ||
| + | |||
| + | |||
| + | Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)" | ||
| + | Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga" | ||
| + | Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor" | ||
| + | Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student" | ||
| + | Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair" | ||
| + | Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil" | ||
| + | Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress" | ||
| + | Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)" | ||
| + | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
| + | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | index = Math.floor(Math.random() * Q.length); | ||
| + | |||
| + | document.writeln(Q[index]); | ||
| + | |||
| + | } | ||
| + | |||
| + | </script> | ||
| + | |||
| + | </head> | ||
| + | |||
| + | <style> | ||
| + | #newquote {color:gray; font-family:verdana; font-size:11px; text-align:right; padding:5px;} | ||
| + | |||
| + | #newquote i {display:block; color:black; font-family:georgia, trebuchet ms; font-size:13px;} | ||
| + | |||
| + | #newquote small {display:block; text-align:right; margin-top:10px;} | ||
| + | |||
| + | ]]></b:skin> | ||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <div id='newquote'> | ||
| + | |||
| + | <script language="javascript"> | ||
| + | |||
| + | makeQuote(); | ||
| + | |||
| + | </script> | ||
| + | </div> | ||
| + | </head> | ||
| + | </html> | ||
Latest revision as of 00:14, 30 August 2011
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19 August 2011
Synthetic DNA sequences status
Objective: Check the size of synthetic DNA sequences.
- Ladder: 100-10.000 bp (Fermentas)
- Agarose concentration: 0.8%
- Samples:
- GEL 1:
- 1. Const. Promoter1 (nd)
- 2. Const. Promoter1
- 3. flhDC1 (nd)
- 4. flhDC1
- 5. flhDC2
- 6. QseB-QseC1 (nd)
- 7. QseB-QseC1
- 8. RBS-SoxR 1 (nd)
- 9. RBS-SoxR 1
- 10. RBS-IL10 1 (nd)
- 11. RBS-IL10 1
- 12. RBS-IL10 2
- 13. RBS-IL12 1 (nd)
- 14. RBS-IL12 1
- 15. RBS-IL12 2
- 16. Ladder
- GEL2:
- 17. Ladder
- 18. RBS-HylB 1 (nd)
- 19. RBS-HylB 1
- 20. RBS-HylB 2
- 21. HylA 1 (nd)
- 22. HylA 1
- 23. HylA 2
- 24. RBS-HylD 1 (nd)
- 25. RBS-HylD 1
- 26. RBS-HylD 2
- Results:
| Gene | Total size (gene+vector) | Linear vector size | Gene size | Result |
|---|---|---|---|---|
| Constitut. promoter | 2795 | 2760 | 35 | Vector size Ok. No promoter band visible. |
| flhDC promoter | 2926 | 2800 | 126 | Vector size Ok. No promoter band visible. Parcial digestion? |
| RBS+HlyD | 4296 | 2800 | 1496 | Fragment size Ok. Parcial digestion. |
| RBS-IL10 | 3345 | 2800 | 545 | Fragment size Ok. Parcial digestion. |
| RBS-IL12 | 4458 | 2800 | 1658 | Fragment size Ok. Parcial digestion. |
| RBS+HlyB | 4983 | 2800 | 2183 | Fragment size Ok. Parcial digestion, where´s linear vector? |
| RBS+HlyA | 3027 | 2800 | 227 | Fragment size Ok. Parcial digestion. |
| RBS+SoxR | 3323 | 2800 | 523 | Fragment size Ok. Parcial digestion. |
| RBS+QseB+RBS+QseC | 4886 | 2800 | 2086 | Fragment size Ok. Parcial digestion, where´s linear vector? |
- Repeat digestions with less DNA.
