From 2011.igem.org

Revision as of 09:04, 25 August 2011

Can you feel the smoke tonight?

Question 1

Would any of your project ideas raise safety issues in terms of:

• Risks to the safety and health of team members or others in the lab

No, the use of certain toxic or carcinogenic chemicals is indispensable in biology lab work. Acetaldehyde is used to turn off our expression system and is a toxic and carcinogenic substance. Work with acetaldehyde is done in a cold room at 4 degrees after cooling down the substance and all equipment to -20 degrees to prevent vaporization. Handling with other volatile chemicals is performed under a hood to avoid inhalation.

E. coli strains DH5-alpha and JM101 are used for our experiments. Both strains are neither pathogenic nor infective nor toxic. Likewise our included constructs do not cause any risk. All lab work is carried out in a S1 lab with conventional safety standards. Normal safety precautions such as wearing gloves, glasses and a lab coat to protect ourselves are implemented. No bacteria are released into the environment, all material contaminated with bacteria is autoclaved at 120°C. Harmful chemicals are collected and disposed of, separately.

• Risks to the safety and health of the general public and Environmental quality if released by design or accident

Our system is isolated and has no direct contact with the environment and other organisms. Thus it should not pose a risk to public or environment by design. A release of genetic modified organism has always a potential risk especially in case of an accident. Gen transfer between the GMOs and other bacteria can always take place. Apart from this risk our system has no additional risks for general public and environmental quality.

• Risks to security through malicious misuse by individuals, groups or states?

Our system is a detector as an output it has only the expression of fluoresced proteins (gfp and mcherry). We could not find any malicious misuse for our system.

Question 2

Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

• did you document these issues in the Registry?
• how did you manage to handle the safety issue?
• How could other teams learn from your experience?

No, the new BioBrick parts we are making do not raise any safety issues.

Question 3

Is there a local biosafety group, committee, or review board at your institution?

• If yes, what does your local biosafety group think about your project?
• If no, which specific biosafety rules or guidelines do you have to consider in your country?

The security consultant of the D-BSSE department of the ETH stated that there is no general security issue with our project and forwarded us to Professor Sven Panke, for getting his opinion on the safety of our biological system. He did not find any reason for concern in our design.

All people working in the lab had a special training on chemical and biological safety in the lab and a fire prevention course in previous practical courses. Also everyone attended lectures on lab safety.

In particular for our project we consulted the "Freisetzungsverordnung" (FrSV) and the "Einschliessungsverordnung" (ESV) in the Swiss Legislature and checked that our project does not raise any safety issues.

Question 4

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

The release of genetically modified organisms (GMOs) to the environment has to be avoided under all circumstances. Thus strains have to be engineered in an appropriate way. Further lab practices need to be optimized and adapted to recent progress done in science. The generation of knowledge and the responsible and sustainable application of it are crucial for the safety of biologically engineered systems.

Safety considerations in Switzerland and at ETH Zurich

References

[1] iGEM 2011 Safety Page

[2] [http://www.bafu.admin.ch/biotechnologie/02618/index.html?lang=en Swiss Legal Bases Biotechnology]

[3] [http://www.admin.ch/ch/e/rs/814_911/index.html Swiss Legislature: Release Ordinance, RO / Freisetzungsverordnung, FrSV]

[4] [http://www.admin.ch/ch/d/sr/814_912/index.html Swiss Legislature: Einschliessungsverordnung, ESV (german / french / italian only)]

[5] [http://www.efbs.admin.ch/en/index.html Swiss Expert Committee for Biosafety SECB]

[6] [http://www.sicherheit.ethz.ch/index_EN SSHE: Safety, Security, Health and the Environment @ ETH]