Team:UNICAMP-EMSE Brazil/Notebook/22 August 2011

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Template:UNICAMP-EMSE Brazil_Notebook}} <div style="text-align: left;"> [[Image:UNICAMP-EMSE Brazil Gel 22 08.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 1, ...")
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*'''Samples:'''  Terminator/ Terminator / RBS+GFP/ RBS+GFP / RBS+HlyD/ RBS+HlyB/ RBS+TolC/ RBS+HlyA /RBS+SoxR /RBS+QseB+RBS+QseC
*'''Samples:'''  Terminator/ Terminator / RBS+GFP/ RBS+GFP / RBS+HlyD/ RBS+HlyB/ RBS+TolC/ RBS+HlyA /RBS+SoxR /RBS+QseB+RBS+QseC
-
*'''Picture legend:'''
+
:*RBS+HlyA did not appear in the gel!! Digest and purify again !!!
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#'''Gel:''' flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)
+
<div style="color:blue">'''Nd: non digested'''<br>'''D: digested'''
<div style="color:blue">'''Nd: non digested'''<br>'''D: digested'''
</div>
</div>
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*<div style="color:#f5faff">'''Fragment lengths:'''<br>
 
-
:*''flhDC''- 126 bp
 
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:*''SoxS'' – 103 bp
 
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:*''Constitutive'' – 35 bp
 
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:*''Constitutive + mRFP'' – around 700 bp
 
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<br>
 
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*'''See below the representation of the plasmid [http://partsregistry.org/Part:BBa_J23119 BBa_J23119] containing mRFP:''' <br>
 
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<br>[[Image:PLASMID_BBa_J23119.png|center|link=http://partsregistry.org/Part:BBa_J23119]]
 

Revision as of 12:15, 23 August 2011

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Notebook

Click on a date to see what we have done!

May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_May_2011&action=edit 1]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_May_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_May_2011&action=edit 8]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_May_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_May_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_May_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_May_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_May_2011&action=edit 15]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_May_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_May_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_May_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_May_2011&action=edit 22]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_May_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_May_2011&action=edit 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_May_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_May_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_May_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_May_2011 31]
June
MTWTFSS
    [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_June_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_June_2011 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_June_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_June_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_June_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_June_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_June_2011&action=edit 12]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_June_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_June_2011&action=edit 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_June_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_June_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_June_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_June_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_June_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_June_2011&action=edit 26]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_June_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_June_2011&action=edit 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_June_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_June_2011&action=edit 30]
July
MTWTFSS
        [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_July_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_July_2011&action=edit 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_July_2011 3]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_July_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_July_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_July_2011&action=edit 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_July_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_July_2011 11] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_July_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_July_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_July_2011 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_July_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_July_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/18_July_2011 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_July_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_July_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_July_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_July_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_July_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_July_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_July_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_August_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_August_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_August_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_August_2011 19] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_August_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_August_2011 21]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_August_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_August_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_August_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_August_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_August_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_August_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_August_2011&action=edit 28]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_August_2011 29] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_August_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_September_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_September_2011&action=edit 4]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/5_September_2011 5] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_September_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_September_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_September_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_September_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_September_2011 10] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_September_2011 11]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_September_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_September_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_September_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_September_2011&action=edit 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_September_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_September_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_September_2011&action=edit 18]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_September_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_September_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_September_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_September_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_September_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_September_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_September_2011 25]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_September_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_September_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_September_2011 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_September_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_October_2011&action=edit 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_October_2011 2]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_October_2011&action=edit 31]


Electrophoresis Gel 1, 21/08/2011.
Gel 1: flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d) .

21 August 2011

Objective: Cut the band of the opened vector (terminator) or cut gene (all the rest);

  • Ladder: 100 bp Bio-Rad
  • Samples:
  • (all digested, forgot to apply the closed vector sample in the gel) Terminator/Terminator/RBS+GFP/RBS+GFP/RBS+HlyD/RBS+HlyB/RBS+TolC/RBS+HlyA/RBS+SoxR/RBS+QseB+RBS+QseC
  • Results:
Gene Total size (gene+vector) Linear vector size Gene size Result
Terminator ~3300 pb 3300 pb No cut Ok, complete digestion
RBS+GFP ~2800 pb 2079 pb 735 pb Ok, complete digestion
RBS+HlyD 4296 pb 2800 pb 1496 pb Ok, complete digestion
RBS+HlyB 4983 pb 2800 pb 2183 pb Parcial digestion, where´s the linear vector? superposition?
RBS+TolC 4380 pb 2800 pb 1580 pb Ok, complete digestion
RBS+HlyA 3027 pb 2800 pb 227 pb Ok, complete digestion
RBS+SoxR 3323 pb 2800 pb 523 pb Ok, complete digestion
RBS+QseB+RBS+QseC 4886 pb 2800 pb 2086 pb Parcial digestion, where´s the linear vector? superposition?

[edit]

All the genes were purified using Fermentas kit (25 uL elution) and quantified through electrophoresis:

  • Ladder: 100 bp Bio-Rad
  • Objective:
  • Quantification (volume of ladder applied 6 uL; sample: 5 uL DNA +1 uL buffer )

Electrophoresis Gel, 21/08/2011.
Gel 1: flhDC C1 (nd)/flhDC 1 (d)/flhDC 2 (nd)/ flhDC 2 (d)/ SoxS 3 (nd)/SoxS 3 (d)/SoxS 4 (nd)/ SoxS 4 (d)/ Constitutive (strong) promotor 1 (nd)/ Constitutive (strong) promotor 1 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d)/ Constitutive (strong) promotor 3 (nd)/ Constitutive (strong) promotor 3 (d) .
  • Samples: Terminator/ Terminator / RBS+GFP/ RBS+GFP / RBS+HlyD/ RBS+HlyB/ RBS+TolC/ RBS+HlyA /RBS+SoxR /RBS+QseB+RBS+QseC
  • RBS+HlyA did not appear in the gel!! Digest and purify again !!!
Nd: non digested
D: digested
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