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Team:Lyon-INSA-ENS/Realisation/Week7Fr
From 2011.igem.org
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Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.<br/> <br/> | Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.<br/> <br/> | ||
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| - | PCR | + | <br> |
| + | Les PCR n'ont pas marché. | ||
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Revision as of 10:56, 5 August 2011
Semaine 7
Du Lundi 25 Juillet au Vendredi 29 Juillet 2011
Lundi
Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.
Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).
Mardi
Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.
Mercredi
Les PCR n'ont pas marché.
Jeudi
Vendredi
La PCR a finalement marché avec la PHU 9104.
