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| | <a name="June.2C_7th"></a><h2> <span class="mw-headline">June, 6th</span></h2> | | <a name="June.2C_7th"></a><h2> <span class="mw-headline">June, 6th</span></h2> |
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| - | <p>These BioBrick were resuspended in 15ul ddH20: | + | <p>RIUNIONE</p> |
| - | </p> | + | |
| - | <ul><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_E2050'>BBa_E2050</A> (mOrange, 744bp) 2010 Kit Plate 2, well 13N in pSB2K3
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| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_K165018'>BBa_K165018</A> (ADH1 terminator, 253 bp) 2010 Kit Plate 3, well 2M, J63009 (Amp)
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| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_K165037'>BBa_K165037</A> (tef2 promoter, 403bp) 2010 Kit Plate 3, well 22O, pSB1AK3
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| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_P1004'>BBa_P1004</A> (Chloramphenicol resistence cassette, 769 bp) 2009 Kit plate 1, well 7B, pSB1A1
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| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_J61001'>BBa_J61001</A> (R6K origin, 406bp) 2010 Kit plate 1, well 240, pSB1A2
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| - | | + | |
| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_K081008'>BBa_K081008</A> (RBS-luxI, 664bp) 2010 Kit plate 2, well 10L, pSB1A2
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| - | </li><li><A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_K125500'>BBa_K125500</A> (GFP fusion brick, 718bp) 2010 Kit plate 3, well 2P, pSB1A2
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| - | </li></ul>
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| - | <p>1,5 ul of each culture was transformed in 100 ul of home-made competent <i>E. coli</i> DH5alpha.
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| - | </p><p>Tranformants were all plated on LB agar plates added with Ampicillin, except for <A HREF='http://partsregistry.org/wiki/index.php/Part:BBa_E2050'>BBa_E2050</A> (Kanamycin).
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| - | </p><p>500 ml LB+Amp was prepared and 21 LB agar plates + Amp were prepared (500 ml).
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| - | </p>
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| - | <div align="right"><small><a href="#indice" title="">^top</a></small></div>
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| - | <p><br /><br />
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| - | </p>
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| - | <table border="0" width="100%" class="menu">
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| - | <tr>
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| - | <td align="left">Previous week</td>
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| - | <td align="right"><a href="/Team:UNIPV-Pavia/Calendar/June/settimana3" title="Team:UNIPV-Pavia/Calendar/June/settimana3"> Next week</a></td>
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| - | </tr>
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| - | </table>
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| | </html> | | </html> |
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| - | {{end}} | + | {{endcalendar}} |
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