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Team:Lyon-INSA-ENS/Realisation/Week2Fr
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Revision as of 13:40, 28 July 2011
Semaine 2
De lundi le 20 juin au vendredi le 24 juin 2011
Monday
One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid
Tuesday
4 more new clones have grown : start of 5mL liquid cultures on these clones.
Wednesday
Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)
Thursday
PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)
Friday
