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JULY: WEEK 4
July, 18th
Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours; meanwhile two medium-size agarose gels were prepared according to protocols. Moreover In the afternoon gel electrophoresis was performed.
2 righe sopra inserire l' immagine As shown in figure, all clones were positive (apart from E13 run where it is possible to recognize only a band at approximately 2800 bp, corresponding to the undigested plasmid), so we cut and purified the bands of interest. Cells harbouring E13 were again inoculated in 5 ml LB + Amp. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Ligations were incubated ON at 16°C. July, 19th
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Team:UNIPV-Pavia/Calendar/July/settimana4
From 2011.igem.org
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| - | As shown in figure, all clones were positive (apart from E13 run where | + | As shown in figure, all clones were positive (apart from E13 run where it is possible to recognize only a band at approximately 2800 bp, corresponding to the undigested plasmid), so we cut and purified the bands of interest. Cells harbouring E13 were again inoculated in 5 ml LB + Amp. |
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Revision as of 10:05, 21 July 2011
