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JULY: WEEK 4
July, 18th
Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours; meanwhile two medium-size agarose gels were prepared according to protocols. Moreover In the afternoon gel electrophoresis was performed.
2 righe sopra inserire l' immagine As shown in figure, all clones were positive (apart from E13 run where we inexplicably didn't see any band), so we cut and purified the bands of interest. Cells harbouring E13 were again inoculated in 5 ml LB + Amp. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Ligations were incubated ON at 16°C. July, 19th
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Team:UNIPV-Pavia/Calendar/July/settimana4
From 2011.igem.org
(Difference between revisions)
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| - | <td></html> | + | <td></html><partinfo>BBa_I13521</partinfo><html></td> |
<td>Insert</td> | <td>Insert</td> | ||
<td>12.5</td> | <td>12.5</td> | ||
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<tr> | <tr> | ||
| - | <td></html> | + | <td></html><partinfo>BBa_R0040</partinfo>-<partinfo>BBa_J61002</partinfo><html></td> |
<td>Vector</td> | <td>Vector</td> | ||
<td>20.5</td> | <td>20.5</td> | ||
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<p> | <p> | ||
| - | Reactions were incubated at 37°C for three hours | + | Reactions were incubated at 37°C for three hours; meanwhile two medium-size agarose gels were prepared according to protocols. Moreover <partinfo>BBa_I13521</partinfo> plasmid purification and quantification were carried out: |
</p> | </p> | ||
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In the afternoon gel electrophoresis was performed. | In the afternoon gel electrophoresis was performed. | ||
</p> | </p> | ||
| + | |||
| + | <center> | ||
| + | <table border="1"> | ||
| + | <tr> | ||
| + | <td><b>Plasmid</b></td> | ||
| + | <td><b>DNA (ng/μl)</b></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><partinfo>BBa_I13521</partinfo></td> | ||
| + | <td>37.3</td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | </center> | ||
</html> | </html> | ||
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<p> | <p> | ||
| - | As shown | + | As shown in figure, all clones were positive (apart from E13 run where we inexplicably didn't see any band), so we cut and purified the bands of interest. Cells harbouring E13 were again inoculated in 5 ml LB + Amp. |
</p> | </p> | ||
Revision as of 09:58, 21 July 2011
