"
Team:Baltimore/Notebook
From 2011.igem.org
(Difference between revisions)
(→July) |
(→Calendar) |
||
| Line 52: | Line 52: | ||
===Calendar=== | ===Calendar=== | ||
| + | |||
| + | ---- | ||
| + | |||
====July==== | ====July==== | ||
'''Week 2:''' [[Tuesday, July 12]]; [[Wednesday, July 13]]; [[Thursday, July 14]]; [[Friday, July 15]]; [[Saturday, July 16]]; [[Sunday, July 17]]; | '''Week 2:''' [[Tuesday, July 12]]; [[Wednesday, July 13]]; [[Thursday, July 14]]; [[Friday, July 15]]; [[Saturday, July 16]]; [[Sunday, July 17]]; | ||
Revision as of 17:42, 14 July 2011
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Notebook
Run through for abstract and scheduling:
Start with plasmid with taq gene and pst1 gene
Remove pst1 site from taq coding sequence
- In order to remove the restriction site, do site directed mutagenesis (1day)
- PCR
- Digestion
- Transformation
- Screening (1day)
- Dilute DNA
- Colony PCR individually
- Digestion of PCR product with pst1
- Run gel ~2500bp
Add biobrick prefix/suffix to taq coding sequence
- PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
- Run gel
- Cut out of gel
- Cut with restriction enzyme
- Clean up DNA
- Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
- Transformation
Add a promoter, transcriptional terminator, ribosome binding site (RBS)
- Screen colonies (1 day)
- Colony PCR
- Restriction Digestion
- Clean up DNA
- Sequence (1 day)
Make taq protein
Compare it to other enzymes and make sure it works
Calendar
July
Week 2: Tuesday, July 12; Wednesday, July 13; Thursday, July 14; Friday, July 15; Saturday, July 16; Sunday, July 17;
Week 3: Tuesday, July 19; Wednesday, July 20; Thursday, July 21; Friday, July 22; Saturday, July 23; Sunday, July 24;
Week4: Tuesday, July 26; Wednesday, July 27; Thursday, July 28; Friday, July 29; Saturday, July 30; Sunday, July 31;
