Team:Harvard/Results/Acknowledgements

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=Team Acknowledgements=
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__NOTOC__
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=Team Acknowledgments=
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Our project was conceived and designed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li.  The project was made possible by support from the George Church lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology.  Without their valuable guidance and great patience, we never would have been able to achieve what have done so far.
 
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Although the project is the first to utilize several key technologies in novel ways, the following technologies were developed outside of iGEM.  The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al in the Church lab. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al in the Church lab, and the actual oligo synthesis was generously provided by Agilent Technologies.
+
Our project was selected from a pool of many possible project ideas submitted by both team members and advisers. Team members decided to focus on zinc finger proteins because of the technologies (chip DNA synthesis and MAGE) available to us: we believed that developing new zinc finger proteins was a way to use both technologies to their greatest possible extent. '''This project was not an extension or a continuation of any existing work on zinc fingers, in the Church lab or otherwise.''' The original idea of zinc finger proteins was contributed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li.
-
Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.
+
This project was made possible by support from Dr. George Church's lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology.  Without their valuable guidance and great patience, we never would have been able to achieve what have done so far. While our teaching fellows - members of the Church Lab - gave us many opportunities, they also made sure that we achieved our goals and results entirely via our own work: our teaching fellows provided inspiration, team members achieved what we did through our own perspiration.  
-
All project work was done by team members, and we would like to make the following acknowledgements:
+
Although the project is the first to combine several key technologies in novel ways, these technologies were developed outside of Harvard iGEM.  The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al[[#References|[2]]].  Chip-based DNA synthesis method was developed by Sri Kosuri et al[[#References|[1]]], and the actual oligo synthesis was generously provided by Agilent Technologies, a sponsor of iGEM. Lambda red was originally developed by Yu et al[[#References|[3]]]. We would like to make it clear that we do not claim any credit whatsoever for the creation of the technologies used in this project. ''Our project and Foundational Advance proposal rest on our successful use of and novel integration of these multiple technologies in concert, and subsequent application of this system to generate novel zinc finger proteins as a proof of concept.''
-
<b>Kristin Barclay</b> Bioinformatics, chip sequences design and programming, majority of wiki coding and design
+
We owe great thanks to Alain Viel for his flawless management of the logistics of iGEM, including but not limited to providing lab space, ordering lab supplies when we needed them, arranging our trip to the Americas Regional and World Jamborees, and for his excellent taste in footwear. Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.
-
Justin Chew - Bioinformatics, ZF target selection, ZF expression plasmid construction, Wolfe selection system testing
+
Jagesh Shah provided us with excellent advice on both our project and the iGEM competition as a whole: what to expect, how to improve our presentation, and not to take ourselves too seriously. We also owe Jagesh's children Harvard iGEM t-shirts for depriving them of Jagesh's company when several of our meetings ran late into the night.
-
William Clerx - Bioinformatics, ZF target selection, wiki coding and design, video editing, freelance wet lab
+
==Team Member Acknowledgments==
 +
All project work was done by team members, divided according to their skills and expertise, with primary areas of work including:
-
Sarah Fouzia - TolC selection system construction, TolC and Wolfe selection system testing, biobrick construction
+
<b>Kristin Barclay</b><br />[https://2011.igem.org/Team:Harvard/Project/Design Bioinformatics], [https://2011.igem.org/Team:Harvard/Project/Design#Data_and_Analysis chip sequences design and programming], majority of wiki coding and design, [https://2011.igem.org/Team:Harvard/Human_Practices human practices]
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Naomi Genuth - Wolfe selection system construction and testing, biobrick construction and registration
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<b>Justin Chew</b><br />[https://2011.igem.org/Team:Harvard/Project/Design Bioinformatics], [https://2011.igem.org/Team:Harvard/Project/Design#Selection_of_Target_Sequences ZF target selection], [https://2011.igem.org/Team:Harvard/Project/Synthesize#Zinc_Finger_Expression_Plasmids ZF expression plasmid construction], [https://2011.igem.org/Team:Harvard/Project/Test#Wolfe_Selection_System Wolfe selection system testing], wiki coding and design, poster design
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Brandon Gerberich - Bioinformatics, Wolfe selection system construction and testing, interview cameraman and video editor
+
<b>William Clerx</b><br />[https://2011.igem.org/Team:Harvard/Project/Design#Selection_of_Target_Sequences ZF target selection], wiki design, video editing, freelance wet lab, [https://2011.igem.org/Team:Harvard/Human_Practices human practices]
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Mark Kopelman - Wet lab, video editing
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<b>Sarah Choudhury</b><br />[https://2011.igem.org/Team:Harvard/Project/Test#TolC_Selection_System TolC selection system construction and testing] and [https://2011.igem.org/Team:Harvard/Project/Test#Wolfe_Selection_System Wolfe] selection system testing, [https://2011.igem.org/Team:Harvard/Results/Biobricks biobrick construction]
-
Matt Lunati - TolC selection system construction and testing
+
<b>Naomi Genuth</b><br />Majority of [https://2011.igem.org/Team:Harvard/Project/Test one-hybrid selection system construction and testing], [https://2011.igem.org/Team:Harvard/Results/Biobricks biobrick construction and registration]
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Nida Naushad - Bioinformatics, ZF expression plasmid construction, chip library assembly, biobrick construction
+
<b>Brandon Gerberich</b><br />[https://2011.igem.org/Team:Harvard/Project/Design#Selection_of_Target_Sequences ZF target selection], [https://2011.igem.org/Team:Harvard/Project/Test#Wolfe_Selection_System Wolfe selection system testing], interview cameraman and video editor, [https://2011.igem.org/Team:Harvard/Human_Practices human practices]
-
Finally, we would like to specially acknowledge all of our TFs who have always been there for us through thick and thin, no matter how small or big the problem.  Their advice has been of immeasurable value to our project's success.  We would also like to also acknowledge the following TFs for their special contribution:
+
<b>Mark Kopelman</b><br />Wet lab
-
Daniel Goodman - Harvard iGEM logo design, wiki design, and being an all around awesome team leader
+
<b>Matt Lunati</b><br />[https://2011.igem.org/Team:Harvard/Project/Test#TolC_Selection_System TolC selection system construction and testing]
-
Noah Taylor - Using lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical
+
<b>Nida Naushad</b><br />[https://2011.igem.org/Team:Harvard/Project/Design Bioinformatics], [https://2011.igem.org/Team:Harvard/Project/Design#Data_and_Analysis chip sequences design and programming], [https://2011.igem.org/Team:Harvard/Project/Synthesize#Zinc_Finger_Expression_Plasmids ZF expression plasmid construction], [https://2011.igem.org/Team:Harvard/Project/Synthesize chip library assembly], [https://2011.igem.org/Team:Harvard/Results/Biobricks biobrick construction]
-
Srivatsan Raman - Collaborator in our project abstract and Human Practices page
+
==Adviser Acknowledgments==
 +
 
 +
Finally, we would like to specially acknowledge all of our teaching fellows (TFs) who have always been there for us, no matter how small or big the problem.  Their advice has been of immeasurable value to our project's success.  We would also like to also acknowledge the following TFs for their special contributions:
 +
 
 +
<b>Daniel Goodman</b><br />[https://2011.igem.org/File:HARVshield_logo.png Harvard iGEM logo design], wiki design (contributer to CSS and design of the navigation bar), and being an all around awesome team leader
 +
 
 +
<b>Noah Taylor</b><br />Used lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical, contributed a reverse translating function from his own research to bioinformatics
 +
 
 +
<b>Srivatsan Raman</b><br />Collaborator in our project [https://2011.igem.org/Team:Harvard/Project/Details abstract] and [https://2011.igem.org/Team:Harvard/Human_Practices human practices] page
 +
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<div class="whitebox">
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=References=
 +
'''1.''' Sriram Kosuri, Nikolai Eroshenko, Emily M LeProust, Michael Super, Jeffrey Way, Jin Billy Li, George M Church. (2010). Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. ''Nature Biotechnology'', 28(12):1295-9. [http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html]
 +
 
 +
'''2.''' Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). ''Nature'', 460(7257):894-8. [http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html]
 +
 
 +
'''3.''' Yu D., H. M. Ellis, et al. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 97(11): 5978-5983.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165854/]
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Latest revision as of 03:41, 29 October 2011

bar

Team Acknowledgments

Our project was selected from a pool of many possible project ideas submitted by both team members and advisers. Team members decided to focus on zinc finger proteins because of the technologies (chip DNA synthesis and MAGE) available to us: we believed that developing new zinc finger proteins was a way to use both technologies to their greatest possible extent. This project was not an extension or a continuation of any existing work on zinc fingers, in the Church lab or otherwise. The original idea of zinc finger proteins was contributed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li.

This project was made possible by support from Dr. George Church's lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology. Without their valuable guidance and great patience, we never would have been able to achieve what have done so far. While our teaching fellows - members of the Church Lab - gave us many opportunities, they also made sure that we achieved our goals and results entirely via our own work: our teaching fellows provided inspiration, team members achieved what we did through our own perspiration.

Although the project is the first to combine several key technologies in novel ways, these technologies were developed outside of Harvard iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al[2]. Chip-based DNA synthesis method was developed by Sri Kosuri et al[1], and the actual oligo synthesis was generously provided by Agilent Technologies, a sponsor of iGEM. Lambda red was originally developed by Yu et al[3]. We would like to make it clear that we do not claim any credit whatsoever for the creation of the technologies used in this project. Our project and Foundational Advance proposal rest on our successful use of and novel integration of these multiple technologies in concert, and subsequent application of this system to generate novel zinc finger proteins as a proof of concept.

We owe great thanks to Alain Viel for his flawless management of the logistics of iGEM, including but not limited to providing lab space, ordering lab supplies when we needed them, arranging our trip to the Americas Regional and World Jamborees, and for his excellent taste in footwear. Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.

Jagesh Shah provided us with excellent advice on both our project and the iGEM competition as a whole: what to expect, how to improve our presentation, and not to take ourselves too seriously. We also owe Jagesh's children Harvard iGEM t-shirts for depriving them of Jagesh's company when several of our meetings ran late into the night.

Team Member Acknowledgments

All project work was done by team members, divided according to their skills and expertise, with primary areas of work including:

Kristin Barclay
Bioinformatics, chip sequences design and programming, majority of wiki coding and design, human practices

Justin Chew
Bioinformatics, ZF target selection, ZF expression plasmid construction, Wolfe selection system testing, wiki coding and design, poster design

William Clerx
ZF target selection, wiki design, video editing, freelance wet lab, human practices

Sarah Choudhury
TolC selection system construction and testing and Wolfe selection system testing, biobrick construction

Naomi Genuth
Majority of one-hybrid selection system construction and testing, biobrick construction and registration

Brandon Gerberich
ZF target selection, Wolfe selection system testing, interview cameraman and video editor, human practices

Mark Kopelman
Wet lab

Matt Lunati
TolC selection system construction and testing

Nida Naushad
Bioinformatics, chip sequences design and programming, ZF expression plasmid construction, chip library assembly, biobrick construction

Adviser Acknowledgments

Finally, we would like to specially acknowledge all of our teaching fellows (TFs) who have always been there for us, no matter how small or big the problem. Their advice has been of immeasurable value to our project's success. We would also like to also acknowledge the following TFs for their special contributions:

Daniel Goodman
Harvard iGEM logo design, wiki design (contributer to CSS and design of the navigation bar), and being an all around awesome team leader

Noah Taylor
Used lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical, contributed a reverse translating function from his own research to bioinformatics

Srivatsan Raman
Collaborator in our project abstract and human practices page

References

1. Sriram Kosuri, Nikolai Eroshenko, Emily M LeProust, Michael Super, Jeffrey Way, Jin Billy Li, George M Church. (2010). Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nature Biotechnology, 28(12):1295-9. [http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html]

2. Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). Nature, 460(7257):894-8. [http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html]

3. Yu D., H. M. Ellis, et al. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 97(11): 5978-5983.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165854/]