Team:Bielefeld-Germany

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<a href="https://2011.igem.org/Team:Bielefeld-Germany/Labjournal"><img src="http://2011.igem-bielefeld.de/includes/wiki/images/group_foto4.jpg" alt="group_foto4" title="group_foto4" id="wows0"/>Jojojo das ist eine Test-description</a>
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<a href="#wows5" title="Safety"><img src="https://static.igem.org/mediawiki/2011/b/b0/Bielefeld-Germany2011-safety-small.jpg" alt="Bielefeld-Germany2011-safety"/>6</a>
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We care for biosecurity - check out our [[Team:Bielefeld-Germany/Safety | safety page]].
 
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==6th Cebitec Symposium==
 
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'''European iGEM teams meet in Bielefeld, Germany'''
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<h1>The project</h1>
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'''A review on the 6th CeBiTec Symposium Genome-Based Microbiology: From -omics Research to Systems and Synthetic Biology'''
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<p>The development of sensitive and selective biosensors is an important research field in synthetic biology. Biosensors can be applied in a wide range of uses - from the detection of environmental toxics up to clinical diagnostics. Because cells have to sense their surroundings, there are a lot of natural systems that are similar to a biosensor. Prejudicial cellular biosensors often show negative side effects that complicate any practical application. Common problems are the limited use outside of a gene laboratory due to the use of genetically engineered cells, the low durability because of the usage of living cells and the appearance of undesired signals induced by endogenous metabolic pathways.</p>
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The annual CeBiTec Symposium, first held in 2006, deals with prospective topics in the broad spectrum of biotechnological research. Each year international scientists come together in Bielefeld setting the focus on current innovations, approaches and methodologies. The issues so far included ''Solar Bio-Fuels'' (2008), ''bioIMAGING'' (2009) and ''New Frontiers in Microbial Genome Research'' (2010). The 6th CeBiTec Symposium was held under the topic Genome-Based Microbiology: ''From -omics Research to Systems and Synthetic Biology'' took place between the 18th and 20th of July, 2011. As this year’s topic included Synthetic Biology, this offered the opportunity for the iGEM team Bielefeld to add an iGEM session to the symposium’s agenda. The students accommodated five teams from all over Europe to present and discuss their past and future projects in front of the international scientific community.  
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<p>To solve these problems, the iGEM-Team Bielefeld 2011 aims to develope a cell-free bisphenol A (BPA) biosensor based on a coupled enzyme reaction fused to S-layer proteins for everyday use. Bisphenol A is a supposedly harmful substance which is used in the production of polycarbonate. To detect BPA it is degraded by a fusion protein under formation of NAD<sup>+</sup> which is detected by an NAD<sup>+</sup>-dependent enzymatic reaction with a molecular beacon. Both enzymes are fused to S-layer proteins which build up well-defined nanosurfaces and are attached to the surface of beads. By providing these nanobiotechnological building blocks the system is expandable to other applications.</p>
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Starting with one of the last year’s finalist TU Delft’s 2010 iGEM project about the conversion of different hydrocarbon sources in aqueous environments, all attending teams presented their results and strategies. The team from the University of Southern Denmark, Odense, presented their 2010’s project Flow E. The aim was to develop a biological system, which is able to generate flow in a fluid-filled tube by inducing the flagella movement with light by the use of a photosensor. This year’s iGEM Team from the University of Edinburgh aims to use and convert plant biomass, especially cellulose, to fermentable sugars like glucose. Therefore several cellulases are fused to a coat protein of the M13 bacteriophage. Ultimately the team’s goal is to create a biorefinery system to convert complex glucans into higher value products. The last year’s medicine track winner Freiburg presented their virus construction kit for therapy use. They managed to create different BioBricks and devices for individual virus construction. Last year’s grand prize winner Slovenia gave the audience a brief historical overview of all Slovenian iGEM projects so far. The session closed with the talk of the Team Bielefeld dealing with their topics from 2010 – a modulated two component sensor system for detection of capsaicin – and a preview for 2011 – nanobiotechnolgical S-layer based Biosensor for bisphenol A.
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Additionally, the students used the opportunity to share and exchange their iGEM experience and their ideas of Synthetic Biology at the symposium and at the social events provided by the iGEM Team Bielefeld. The symposium’s iGEM session was a success and hence indices that the iGEM idea reached the scientific community.
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<h1>The Bisphenol A-Team</h1>
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'''Some expressions from the CeBiTec Symposium:'''
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Image:6thcebsymp_postersession.JPG| Postersession
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Image:6thcebsymp_delft.JPG| Presention Team Delft 2010
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Image:6thcebsymp_confdinnerfun.JPG|Social event:conference dinner
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Image:6thcebsymp_frei.JPG| Presentation Team Freiburg 2010
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Image:6thcebsymp od.JPG|Presentation Team Odense 2010
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Image:6thcebsymp_conf.JPG|Audience
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Image:6thcebsymp edin.JPG|Presention Team Edinburgh 2011
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Image:6thcebsymp slov.JPG| A brief history of slovenian iGEM Teams
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Image:6thcebsymp np.JPG| Niko and Pieter discussing the interesting topics
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Image:6thcebsymp_attendees.JPG| All attendees of the iGEM session
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Latest revision as of 01:14, 29 October 2011

Bielefeld-Germany2011-team Bielefeld-Germany2011-project Bielefeld-Germany2011-results Bielefeld-Germany2011-model Bielefeld-Germany2011-human-practices Bielefeld-Germany2011-safety
Bielefeld-Germany2011-team1 Bielefeld-Germany2011-project2 Bielefeld-Germany2011-results3 Bielefeld-Germany2011-model4 Bielefeld-Germany2011-human-practices5 Bielefeld-Germany2011-safety6




The project

The development of sensitive and selective biosensors is an important research field in synthetic biology. Biosensors can be applied in a wide range of uses - from the detection of environmental toxics up to clinical diagnostics. Because cells have to sense their surroundings, there are a lot of natural systems that are similar to a biosensor. Prejudicial cellular biosensors often show negative side effects that complicate any practical application. Common problems are the limited use outside of a gene laboratory due to the use of genetically engineered cells, the low durability because of the usage of living cells and the appearance of undesired signals induced by endogenous metabolic pathways.

To solve these problems, the iGEM-Team Bielefeld 2011 aims to develope a cell-free bisphenol A (BPA) biosensor based on a coupled enzyme reaction fused to S-layer proteins for everyday use. Bisphenol A is a supposedly harmful substance which is used in the production of polycarbonate. To detect BPA it is degraded by a fusion protein under formation of NAD+ which is detected by an NAD+-dependent enzymatic reaction with a molecular beacon. Both enzymes are fused to S-layer proteins which build up well-defined nanosurfaces and are attached to the surface of beads. By providing these nanobiotechnological building blocks the system is expandable to other applications.


The Bisphenol A-Team

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