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Team:ETH Zurich/Achievements/Data page
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| - | = Data page = | + | = SmoColi-Data page = |
| - | '''SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red. | + | '''SmoColi cells are engineered to sense toxic substances found in cigarette smoke.''' They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red. |
{{:Team:ETH Zurich/Templates/SectionEnd}} | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
{{:Team:ETH Zurich/Templates/SectionStart}} | {{:Team:ETH Zurich/Templates/SectionStart}} | ||
| + | |||
= How Our System Works = | = How Our System Works = | ||
| - | [[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]] | + | <center> |
| + | <html> | ||
| + | <img src="/wiki/images/a/ac/ETHZ_Data_page.png" usemap="#Datapage1"/> | ||
| + | <map name="Datapage1"> | ||
| + | <area shape="rect" alt="" title="" coords="217,122,433,164" href="#Our_Favorite_New_Parts"/> | ||
| + | <area shape="rect" alt="" title="" coords="217,169,433,186" href="#Characterized New Parts "/> | ||
| + | <area shape="rect" alt="" title="" coords="0,260,716,296" href="#Our_Favorite_New_Parts"/> | ||
| + | <area shape="rect" alt="" title="" coords="464,94,532,130" href="#Our_Favorite_New_Parts"/> | ||
| + | <area shape="rect" alt="" title="" coords="216,192,434,252" href="#Characterized_Pre-existing_Parts"/> | ||
| + | </map> | ||
| + | </html> | ||
| + | </center> | ||
| + | <!--[[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]--> | ||
{{:Team:ETH Zurich/Templates/SectionEnd}} | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
{{:Team:ETH Zurich/Templates/SectionStart}} | {{:Team:ETH Zurich/Templates/SectionStart}} | ||
| - | = | + | = Our Favorite New Parts = |
| - | #[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | + | #[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]]) |
| - | #[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation# | + | #[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]]) |
| - | #[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' | + | #[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]]) |
{{:Team:ETH Zurich/Templates/SectionEnd}} | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
{{:Team:ETH Zurich/Templates/SectionStart}} | {{:Team:ETH Zurich/Templates/SectionStart}} | ||
| - | = Characterized | + | |
| - | #[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | + | =Characterized New Parts = |
| - | #[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation# | + | #[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]]) |
| + | #[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]]) | ||
| + | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
| + | {{:Team:ETH Zurich/Templates/SectionStart}} | ||
| + | |||
| + | =Characterized Pre-existing Parts = | ||
| + | #[http://partsregistry.org/Part:BBa_I13453:Experience Experience]-'''P<sub>BAD</sub> promoter''', BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P<sub>BAD</sub> in BW27783 ([[Team:ETH_Zurich/Biology/Validation#AraC_-_the_arabinose_sensor|Characterization]]) | ||
{{:Team:ETH Zurich/Templates/SectionEnd}} | {{:Team:ETH Zurich/Templates/SectionEnd}} | ||
{{:Team:ETH Zurich/Templates/HeaderNewEnd}} | {{:Team:ETH Zurich/Templates/HeaderNewEnd}} | ||
Latest revision as of 23:59, 28 October 2011
Contents |
SmoColi-Data page
SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
How Our System Works
Our Favorite New Parts
- [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
- [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. (Characterization)
- [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)
Characterized New Parts
- [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
- [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)
Characterized Pre-existing Parts
- [http://partsregistry.org/Part:BBa_I13453:Experience Experience]-PBAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the PBAD in BW27783 (Characterization)
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