Team:ETH Zurich/Achievements/Data page

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|style="font-size:2em; height: 30px" class="modeling"|Data page
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|style="border-left: none;"|[[#How Our System Works|How Our System Works]]
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|[[#We've also characterized the following parts|Characterization]]
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|colspan="2"|SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
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== How Our System Works ==
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[[File:ETHZ Data page.png|800px|left|thumb|'''Figure 1: Molecular mechanism of SmoColi with xylene sensor,''' depending on the xylene input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]
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== Data For Our Favorite New Parts ==
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#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]])
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= SmoColi-Data page =
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#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy number test|Characterization]])
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'''SmoColi cells are engineered to sense toxic substances found in cigarette smoke.''' They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
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#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor  |Characterization]])
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== We've also characterized the following parts ==
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= How Our System Works =
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#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]])
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<center>
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#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor|Characterization]])
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<img src="/wiki/images/a/ac/ETHZ_Data_page.png" usemap="#Datapage1"/>
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<area shape="rect" alt="" title="" coords="217,122,433,164" href="#Our_Favorite_New_Parts"/>
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<area shape="rect" alt="" title="" coords="217,169,433,186" href="#Characterized New Parts "/>
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<area shape="rect" alt="" title="" coords="0,260,716,296" href="#Our_Favorite_New_Parts"/>
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<area shape="rect" alt="" title="" coords="464,94,532,130" href="#Our_Favorite_New_Parts"/>
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<area shape="rect" alt="" title="" coords="216,192,434,252" href="#Characterized_Pre-existing_Parts"/>
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</center>
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<!--[[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]-->
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{{:Team:ETH Zurich/Templates/SectionEnd}}
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{{:Team:ETH Zurich/Templates/SectionStart}}
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= Our Favorite New Parts =
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#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])
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#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
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#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]])
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{{:Team:ETH Zurich/Templates/SectionEnd}}
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{{:Team:ETH Zurich/Templates/SectionStart}}
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=Characterized New Parts =
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#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])  
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#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]])
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{{:Team:ETH Zurich/Templates/SectionEnd}}
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{{:Team:ETH Zurich/Templates/SectionStart}}
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=Characterized Pre-existing Parts =
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#[http://partsregistry.org/Part:BBa_I13453:Experience Experience]-'''P<sub>BAD</sub> promoter''', BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P<sub>BAD</sub> in BW27783 ([[Team:ETH_Zurich/Biology/Validation#AraC_-_the_arabinose_sensor|Characterization]])
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{{:Team:ETH Zurich/Templates/SectionEnd}}
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{{:Team:ETH Zurich/Templates/HeaderNewEnd}}

Latest revision as of 23:59, 28 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi-Data page

SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works


Our Favorite New Parts

  1. [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. (Characterization)
  3. [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)


Characterized New Parts

  1. [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)


Characterized Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_I13453:Experience Experience]-PBAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the PBAD in BW27783 (Characterization)


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