Team:Fatih Turkey/Experiments6

From 2011.igem.org

(Difference between revisions)
Line 568: Line 568:
         <div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;">
         <div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;">
             <ol>
             <ol>
-
               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Confirmation</a></li>
+
               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li>
                
                
               </li>
               </li>
Line 575: Line 575:
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
                    
                    
-
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
+
                    
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
 +
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
Line 584: Line 585:
         </div>
         </div>
         <div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;">
         <div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;">
-
            <p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p>
+
         
-
<br><br><br><br><br><br><br><br><br><br>
+
<table width="606" height="227" border="0">
 +
  <tr>
 +
    <td width="600" height="223" valign="top"><p><strong>Reflectin  Existence Assay</strong><br />
 +
      We transfered the plasmid which includes the gene part of reflectin protein  (BBa_K541596) to E. Coli and we saw that there is a difference between  reflectin including colonies and the control group.<br />
 +
      We saw that the plate which does not include reflectin protein is normally  transparent. But the plate with the reflectin synthesizing bacteria seemed  white. This makes us think that our plasmid is working and the protein is  produced by E. coli.<br />
 +
    Additionally,  the colonies are looked through light microscope. The colony which was supposed  to produce reflectin had some transparent points. In contrast, the control  group had completely different image. We think that reflectin protein caused  these points; thus reflectin might be synthesized. However, we did not image  the effect of reflectin; so, we do not know whether reflectin works as it is  expected.</p></td>
 +
  </tr>
 +
</table>
 +
 
         </div>
         </div>
     </div>
     </div>

Revision as of 17:01, 28 October 2011

deneme baslik

Experiments

Existence of Reflectin

Reflectin Existence Assay
We transfered the plasmid which includes the gene part of reflectin protein (BBa_K541596) to E. Coli and we saw that there is a difference between reflectin including colonies and the control group.
We saw that the plate which does not include reflectin protein is normally transparent. But the plate with the reflectin synthesizing bacteria seemed white. This makes us think that our plasmid is working and the protein is produced by E. coli.
Additionally, the colonies are looked through light microscope. The colony which was supposed to produce reflectin had some transparent points. In contrast, the control group had completely different image. We think that reflectin protein caused these points; thus reflectin might be synthesized. However, we did not image the effect of reflectin; so, we do not know whether reflectin works as it is expected.
2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Experiments6"