Team:ZJU-China/BBa K561000.html

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href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a></div>
href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a></div>
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<h4>Sugarfilm</h4>
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<h4>Xfilm</h4>
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<div class="pane"> <a href="https://2011.igem.org/Team:ZJU-China/X-Sugarfilm.html">Sugarfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Sensorfilm.html">Sensorfilm</a><a href="https://2011.igem.org/Team:ZJU-China/X-Gluefilm.html">Gluefilm</a></div>
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href="https://2011.igem.org/Team:ZJU-China/Sugar-background.html">Background</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Design.html">Design</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Modeling.html">Modeling</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Future.html">Future</a></div>
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<h4 class="current">Parts</h4>
<h4 class="current">Parts</h4>

Revision as of 13:12, 27 October 2011

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Parts-BBa_K561000

Biofilm

Rainbofilm

Xfilm

Parts

Achievements

Tools

BBa_K561000

Device

Characterization

The expression of YFP under aerobic conditions

  The expression of YFP under aerobic conditions is increasing during the exponential phase. With the micro-aerobic promoter, its expression intensity is very low. The highest expression intensity is 1.15. The PoPS of our device is 0.4506.

The expression of YFP under anaerobic conditions

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  Pvgb is a micro-aerobic promoter and it contributes to high expression of YFP under micro-aerobic conditions. It’s expression level at anaerobic condition is relatively low. The PoPS of this device under anaerobic condition is -0.375.

The expression of YFP under micro-aerobic conditions (2.5% oxygen)

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  The expression of YFP with 2.5% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.28. The PoPS of our device at 2.5% oxygen level is 13.9023.

The expression of YFP under micro-aerobic conditions (10%)

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  The expression of YFP with 10% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.24. The PoPS of our device at 10% oxygen level is 2.0653

PoPS of YFP and the expression level of YFP

  The YFP expression device has a micro-aerobic promoter, Pvgb. Its PoPS reaches the highest when the oxygen concentration is 2.5%. Under anaerobic or aerobic conditions,its PoPS is equal to 0 relatively.